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CRIMIC CR01355

Gene wde

CG12340

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:10801735..10801742	47C1	FBti0206910	83282

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TTGAGATCTCGGCGATTATACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCGTCTACGACCAGGTCTT	CGCCGAGATCTCAAATTCTTCG	CGGACGGACAAATGGACG	CCGCACCAGAGTCAACTCAT

 

Amplicon 5'

Amplicon 3'

TCCGTCTACGACCAGGTCTTGGGAACACTGAACGGCCCGAATCGTTCGTGACTATCAACGCCCCGCACAGCGAAGTAATACCTCTGGTTCTCCTGGAACTGATTTAAAGTCACTGCCATTGGCAGCAACATAGCGCTGACATCGCCCACATGCCGCCAGCTATCCGTGCTGGGCTCGTTAATCGTCTCCTGGTATGCATAGATCTGGTACATTACGCACTCTGCGAACCGCGGGCTCGAATCCTCCAGAGTCCAGGATATAACGATACCAGTATCAAGGAGACTAATCCGGATAACGGGTCGCGAAGGAGGCACCTTCCAGGATGGATTGAAGGGCTGGGGAGGAGACGTGGGCAGTGGTGCAGGATGTGAGTATCTTAGACGAGACGCTGGAGGCGGGGTGTTTTCCGAACGTATCTGCATAGTTACGTTTGAGCCCAAGGGACTTTGCTGACCTTTGAATACGTTTGAAAATAAGTACGCCGAATATTTTGAATATAATGATTTTCCAACTCACCTCCTCCATTATTATGGACTATCTGTCGAGCATTTACGCGAGGCAATTGCATTGGAGAAGCTCGCACCACATTTCCGCCTCCTCGTCCTCCTCTCGTCGCTGCTGCAGTCTGAGCATTCCGCTTAAGCATCTCCATACGCAAACGAGCGTTTTGTGCTTGGGCAGCCCTAGCCGCCGCCGCAGCCGCTGCATCATCCTCATCTGTGAGGTCAATGACTGCCTTCTCCTTGGGCTTGGCCGGCGCCAAGCTGGACTCCTGCTGCTGTCCATAGCTGCCCAATTCCGATCCACTGCTGCTAGATGAGATGGGCACCGAGACCGTGGCCCCCGAAAGCGGTGGCTGCTTGGCGCGCACTTTAGTTGTGCACTTCGGCACTTGCTTGGCGGGACTCGAACCTGGCGTGGGACCGGGACTAGGTCGAGGTCTGCAATATGAAACAATTGTAAGTTAGTAATGTGAATATGACATGTTTCTTTTGACGAAGAATTTGAGATCTCGGCG

CGGACGGACAAATGGACGGACGGACATAAACATATTGATTCGACAAGTATATTTTTATATTGGTAACGCTTTTATTTACCCATTTGGGTTTATTACCCCAACTATAAAGATTTTCAATAGCAATAAAATATACTTGAATGTTAAGAAAACAAAATTTTTTCTGGCATATCAATACAAATTGGGAAATAGAATAATAAAATTTAAAAAAAAATCTATGTATGCTTTTATAGCCACTGAGTTCGAGGCGTTCATATGGACACACGGACGGACATGGCCTGATTGATCCAGATAAAAAAAATATATACGGAAACTCTGCCTTCTACCAGTTATATACTCTGTAACGAATCTAGTATACCGTATTACTCTACAAGTAAAAATATGAATCTATTTAGCTAATTGGGTCTGAAACGATTCCCAATAAATTGCTAATTGTAAACTTCTGCACAAGCGTGGAATTCGCTTTTAATCGAAGATCACGATTAGGATTATACTCACAGTAGGAACTGTTGCTGCGCCTGTTGTTGCTTTTGTTGTTGTTGCAGGTGTTGTTGCTGTTGTTGCTGCTGTTGCTGGTGCTGTTTCTGCTGTTGTTTTTGCGCCAAGAAAGCGGCTTCTGAGTTCCGGGCACGCATGACGGTTTTATTAATCGCATTGGCCACCGATGAAGGCGTGGAGCCAGGCGTTCCGGTGTGCTTGGAGGCGTGCATTGTCCTTTGAGCTATAGGCGGTGAGGACTGCAGACGTTGAACGCTGGACTGATTCATAAGCATTGGCGCGGGCTGTAAGTTAGTAGGTACCGGCCGATGGGGCGTGACCTTCTGCAAGCAGCCCCGGCGCACAGGTGGCGTGACACTAGCCGTTGTTAAGGATTGAGATGACCGTGCTGCTGGCTGCTGTTGCTGGTGATTGGAATTCGGAGTGACACTGACGCCCGCACCCGTTTCCGATGAGGATGTGGATGGTCCAAAGGTAGGCGGAGGAGGTCTTGGTCGCACGGTCGCACGCGTCGGGGAGGTGCTGGCCTTGGGTGGTGTTCCGCTGGGCGGCGCCAACACGCTGCCAGCAGCATGAGTTGACTCTGGTGCGG

 

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