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CRIMIC CR01356

Gene Ehbp1

CG15609

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:17014860..17014865	53E4	FBti0206911	83283

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TATTAACCCCTCAAGTTGAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGATAGAGAGGTTCGACGGC	ACTTGAGGGGTTAATAGCACGT	AGGCACTTAAGACCGCCAC	TGTCCCCAAATCGAAGGACC

 

Amplicon 5'

Amplicon 3'

CGATAGAGAGGTTCGACGGCCGCCTGAAATTGGCCATCACATGATTGTTAACCATTTCATGCCAAATTGGTTTCTCAAAGTTCTTTCAACTCTGATCATGTATTTTATATACAAGACCATGAATAACAATTTCTGAACACCGGATGCGGCGTCATTATATGCATATGTGGGTTCTTCAGTTGATAAATATATGCGATGATTGGTTTATTAAACAAAAGTCTGTACAAAACATTTCATGTCAACAGGAAAAGTTGGGGTCATTTGCAAATACAAAGATATAGATAGCAAATGGAGTACGACTATATTATAAATTCGTAGTCATGGAATTCAGTAGCCAATAGCCTCATCAATATTATACTATTTTCAATATTTTGCTTAATTTATTTGCATGACTAACTCCTCTCGGCAATTGAATTAAACTATATAATTAGTTGCTCAATGATATTCTCCGAAATATGTACTTATTTAGATAAAGGTAAATTAAAGGTGCAGAGTAATAAAGGTCAAGTAACTAGTCATATCAATATTTTATCGTATTTATGAAACAATCATTGTATATGAAGGGAAAATAAATCTTCAACTATAAGCGTTTGTTTAGCTTGTTCTTTGAAAATGATAAGAAATATTATAACTTCAACAAATGATACTGCAGTAGGGTCTACACATTTTTTGCGCATAAATATATATACGTATATATTCATTAATAAAAATAGACGCGAATGCAAGATAAGGCAAGTAAATATAAACATTAATAAGAAGATATTCTTTTGAAGAAACACAAAAATTACATGGAAACGGAAAAAAATATATTGATTTCAATGATACACAAAAAGGAATCTAAAAACGAGATATCTTTCCGCCAGAAACGTGCTATTAACCCCTCAAGT

AGGCACTTAAGACCGCCACCGACCCGCCCCGCCCACTTTAGCCGACTCCCGGTGAACTAATCCACTTTCATGTGGGGCACTTACCACTTGGCGGTGGTTTCCAGATGCAGATCGTGGTACGATGCGGTGAACTGGAATTTGGCGGCTCGCTTGAAATTGCGCTGCAATCGCTTCCATACGCTGGCCATCTTAGCGGGTGTGGATCGTAGGGCTCTTTAATTTGGATATCTTTGGGGGTGTTGGGGTTTGTATAAAAATTAGACCCGTTTGGATCGTCGTTGCTTTGCTTTTTCAGTAATCTATTGTGTCATTTTTACCAGCTAACTTCTGCAGGCGATCTGGATGCGGAATGAAAAACAATATAGACGCCTGGTTATATGCTATAAATTTCTGGGTCAGTTTACAAGGTTCATTCATAAGGGAACCAAAAAAAAAAGGCAGACCCTCTTAAAATCGAAAGTTAGAGTGTGCAAGGCGGAAAACACTATTGTAAATTTATTTTTGAATTTTGTTGCTTTGTTGTCTGCAAAAACACTCAAAAAACACACGCACACACACGCGCGCACAGTTCTTGTGTTGTGTTGTATTTGTTTTTCTTTTTCGCTGTTTTGTGACCTTTAGTTGTCACTTTGCCTTTGTCTTTCTTGAATTTCTTATAAATACTCGAGATTTACAGCTTATTGCTAGTTTTGTTTTTATCTATTATTGTTCCTGCGGGCGTGTGTTTGTGTGTTTGCGTCTCACTGGCAAATTTTTAAGCGAAATTTGTATACATAGATGATGCCTAGACGTACATACAAATACATAAATAGCACCATTATGACGTCACTTTTGTTTTGGTCTCCAATTTCGTTTCAAGGTCCTTCGATTTGGGGACA

 

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