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CRIMIC CR01357

Gene knon

CG7813

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:16499118..16499131	53C6	FBti0206912	83284

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band

Images

Construct Design Sequences

sgRNA sequence	GAATTAAGTCTACCTTAGAAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCCATTCCTCCGCCTCTTTT	GAAGGGCAGGGTCTTTACTAA	AATTCATGCTCCCCCAGGTG	GTGGTGAGAGGACATTCGCA

 

Amplicon 5'

Amplicon 3'

CCCATTCCTCCGCCTCTTTTAATTGTCTCTTGCGCTCCAATGTCCTAGCTATCTCCCTGGCTCGCTCCTCCTGGGCATCGCAAGGATCCGCCTCAATGGAAAGGTTAGTATGCCTGGCCTTAACCTCCGGATTGGCCTCTGCCTCCTCGCTGCGTATTATAGTTTGGTTGCAGATGTTCGGTCCAGACTCTCGTTCCGTAATGCTGTCCAGTGACTTGGTTGGTGCGGCTGTAGGCGTTGCGGATTTGCTGGCAGTTTCGGAACGCGCAGCTTTGGCGGCGTCTGCCTTTTTCTGAACATTCTTGGCCAGACCCGACATCTTTTCTTTAAGACTATTAAAAAGAACAATAGCTTTGGCCATCAAATCTTTGGCGATTAAAGTGGCCTTTTCGGCCAACTGAGAGGTGTCGACAACGGGTTCTTTTGGCTCCTTTGGAGCCGCAGCCGCCGGAGGCTTTCCTTCTCCACTAGGCTTATCTGGTAACTTTTCGGGAGCTGGTTTCTTTGGCGGTTCGGGCTCCTCTTCGTGATGCAAAGCAGCTGCTGCCTCTGGATCCGATGGACCTGGCATTTGCTCTTCAGGAGTGTGTGGCCAGAAAGTGGGCTTGTTGATGAAATCGGGTGCCAAGTGCGGACGGGCAAAACAAAATTCCTCCATGGTCATTTCGGAATAGGGTCGCATTGCCTTCACGAACTCCAACTTTTCGGTTAGCTCCTGGATTCTTTTATCCGACTCCGCCTTGTATTCGCTAATACATTTTGTCACCACCTTCTTTTCTTCATCGACGAGTTCAAAGTATCGCTTGTGATCTACTATGGCATGTCGATTCTCGAATACGGAATGCAATTTATCATACTTGGTCTCGTATCCCTTCACCCAATCGACAAACTCTTCTCTCACATTCTGACGGTAGTATTCCCAGTCGATTGTCGGCATCGATTCCGGATACTTACGAACGCGGCTATAAAATATATTTATATTATTCTCTACAATGGGAAATAATTCTCATATAAAGCAACATAAGCTGTAACCTAAATAATTAGTAAAGACCCTGCCCTTC

AATTCATGCTCCCCCAGGTGACCCCAATTCCCTGTTTTTGGTGACCCCATCTTACTCCTTGTACTCCTCGTGCTTCTTGGCAAACATTTTGAACTTGTGCATCTGGTTCGGCGGCACCTGTTTCATCAGATCCGCCAGTTCGACAACTTGGCAGCTTACATTCTTGACGCCACCATTGCCACCATTGCTCTTGATGCAGTTCACCAGCTTTTCCCACATCCTTCGGATTCTTTAGCAGTATTTTTATGGATTTATATATGTGTATGTTCAGGTGTGTTCACATTGGCCTCCAGTCTGTTCTATAACTTGTACAAATATGTGAGTGCGTAGTGTGTGTGTGTGTGTTGAAAACTCCTAAGGAGCCAACTGAGAGCCATTAAAAGTGAAAATTCCAAAAGGCTATAAAAAAAAACGGGGTGGGCGGAGAAATCGAGATTTCAATTTATAGCCCGAAATTGCATATTCATGCAGTTTCAATTAAATCAGAGTCAGGCGCAGCGACAGCTAAGCAAGTGGGTTTGACTAACTGACAATAAAATCGAAAATTAATTCCAACCCAGCAGACTGCTCAAGGAAAGTTAAATGTAATTGCATGGAGATGGGGTATTTTTGGGGTGGCATTGCCAAGTGGGTGGAGTGGGTGGAAATGTAAAATCACACCCAGGATATGCGTATGTGTTTGGGACCTCTCGAGTCTGCGAATGCTCCAAAGTGACAAACGCAATTTATAACGCAACGAAAATGGGCTAATGCCAATTCTTCAGGTAATTGTCGTCTGCTCTCTCTCTCTCTCCCTCTCACTCTCTTTCTCTAGAGAATCATGGTGCGAGAGAGGGTGTGCGAATTGCAACCCCTGCTGGAATCGCAGGATATGCTCCTTTAAACGTTTTGGAACGAAACGAAACATGCGCCTGCGCTCTGTGAATGTTTCGCTGTCATTTTGACAGTTGCGAATGTCCTCTCACCAC

 

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