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CRIMIC CR01358

Gene CG4586

CG4586

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:6971043..6971048	6E4	FBti0206913	83285

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AAACACATTCAGCTCACCCAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAGGAGCGCTACGAACAGAC	GCTGAATGTGTTTTATGGGCT	CCAGGGTTCTTTAGATAATAAT	GAGACATTCGGGCTAAGATC

 

Amplicon 5'

Amplicon 3'

AAGGAGCGCTACGAACAGACCGTGGCCCGATCCACACGCTTCCTGGCCAAACTGCGCCAATGGCAGCGGGAGAAGCAGCCACAACTTCAGCTTAGTGTGAATATGCTGAGGGACTTTCGGCTGCTACTCTCCGGATCCCTGGGCACGGGCCTCTTCCAGCAGAGCTTCCCCCTTCGGGTGCATTTCTCCATGTTCATGACCACCTTGCTGAGCCAGGGAACCGAGGAGCAGCAGGCCCAGTGGCTCAATCGATCCTGGCACATGGACGGCGTCCTGGGCACCTATGCGCAAACGGAGCTGGGTCACGGCACCTTTGTCCGGGGCCTGGAAACCCGAGCCGACTACGATCCCATCACGCAGGAATTCATCCTGAACACCCCAACGCAAAGTTCATACAAGTGGTGGCCCGGCGGACTTGGTAATACCGCCAATGTGGCCATTGTGCTGGCCCAGCTATATGTGAAGGGCAAGCATTATGGCTTGCAATCATTTGTGGTGAGGATCAGGGACGAGAGGACACACGAACCGTTGACTGGAGTCGATGTGGGCGATATAGGACCTCGCCTGGGTGGCAATGGCGTTAACAACGGCTTTCTGGGACTCCGCGACGTGCGGATACCGCGCAATCAAATGCTGATGAAGAACGCCCAAGTCCTGACCGACGGCACCTTCGTCCAGGGTAGACCTCCGCTGCTGCTCTACGGCACAATGGTCTATGTGCGGGTAATTACCGTGAAGGATGTGCTCTTTGGACTGCTCCAGGCTGCCACCATTGCCACCAGGTGATTATATAGTTTCTGGGGATTATGTTTATCATAGCTGTTATTAAGCCCATAAAACACATTCAGC

CCAGGGTTCTTTAGATAATAATTATCATTATCTTTAGGTATTCCGTGGTGCGTCGCCAGAGCCGGATTAACAGCGATCAGCCGGAGGTCTCTGTTTTGGATCACATCACACAGCAGGCAAAGATTCTACCGCAAATTGCTCGAGGAGTGTCCTATCGCCTGGTATCCGATTGGCTGTGGCGCTTCTACGAGGACGTACTCCGCCAACTGGAGGATGAAAGCACGAAAGGACGCAACTCCCTGCCGGAGCTGCACGCCCTGAGCTGCTGCCTCAAGGCGGTGGCCACCGACGAGGCCAGCGAGGGAGTTGATCTGCTGCGCAAGAGTTGCGGTGGCCACGGATTCCTTTCCTCGGCGAATTTCGATAGCATCTATGGTCTGACCGCCGCCACATACACCTACGAGGGAGAGTACACAGTACTCCTGCTCCAGACAGCTCGTTTCCTCGTCCGGCAGTATGCGGATAGCTTAAAACGCAAGGTGCTGCCCTCCAGTGTCTCCTATTTGAGGGATACAGCCCGTTTGATTTGGGGCAAGAATCTGGTTGCGAATTGTGTGCGAGCGCTGGAGATTTCAGCCATGGAGCAGGTGCGTGATGCTTGGCAGACCCAGAAGATGCACCAATCCGGCAAGGTGAGTCCCGAGATGGCCAGCAATCTGGCTGGAAGGCAGCTCACCTCGGCTGCCATAGCCCATGCTCACGCATTCTTCACACGCAATGCACTGGAGCAAGTGGAAGCACTTCGAAAGAAGGGCGATCTTAGCCCGAATGTCTC

 

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