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CRIMIC CR01359

Gene TTLL4B

CG3964

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:3864459..3864478	24C8	FBti0206914	83286

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GGAAGGGGCGAAAGCGACATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGCAGTCAACAGCATTGCA	CATGCTCGTTAGTGGGGTTT	CTTTCGCCCCTTCCTTTCCA	ACCCACTCTTGATGATGCGG

 

Amplicon 5'

Amplicon 3'

GTGCAGTCAACAGCATTGCAGGTGTTCGTTCAATAAAATAAGAGTTTCTTAAATAGAAGAAAAACAGTGCTTTCAACTAACCGAATCATTGTTTAAAAAATAGCGGAATCTTAAAGTGAAAATTGAAACATTTCCAAATAATAATTCCAAACGACAAATATTCAATGAAATAAATCCTGTTTTTATACCTGTTTTAATCACTTGCAGTTATCCAAATTACTTGTGAACTCAATATGGCTCAGATGACCGAGAAGAGGACAACGTCGGCCCGATCTGGTACTGCCGCCAAGGAGAACCACAGCCTGACGGGACGGCAGAACCAGCAGAATCATCACAATCAGGGACACCAGCGCAGCGGCAAGGGCATCGAGATACTCGACTTCCACGAATCGTGGCTGGAGGAGGTGGAGCTGTACAAGGACAAGATGGTCATGTGCAAGAAGCTGGCCCATCACAATCACGTGCCCGCCTCATCGCACCACCTGCAGCCGCACCTGGCCGCCCCGAATCGAAATCATGGCAGAATCGTCCAGTCCAGCACCCCGCAGCATCGCAGCTCGGCCGGCGGAGGATCCGCGGCTGGAGCCACAGCATCCGATTCCGTCTCGGCTCGCTACTCGCCGCCTGGTCAAAAGCAAAGATCACCGCCCTCGCCGCGCAAACGGCTGACGCCGGAGAAGGTGGCCAACTTAGGAGGAGGAGGCGGTGGCTCTAAGGTAGGCGCGCAGCGCATCATCATCCGTGGAACGCCGCCCAACAGCTCCACACCCAAGGTAGCCGCCACGCCCTCAAATGTTCCCGTCGTGGCCAACAGTGTGCTCCAATCGGAACCGTACAAGAAAGTCCAGGTGAGCGCGACTCCTGACTATTTGTTTGTATCTCTGTAATCATCGGGGGAAATCACTTTCTGTGTCTTTATTTATTTATTTAGCGTTGCCTCTTTGAAAGTATTTCTACGTTTGTCATTTAATTAATTTAACATCTTTAACATCTCTATGCTTAATAATACGATATGCATTATCATGTTCAATGACTAATCGAGATATCTCTTGATTCCTATCAACCACATAATTTAATATCTCTCCGTTTATAAAACCCCACTAACGAGCATG

CTTTCGCCCCTTCCTTTCCATGTTGTGTGTTTCCGTTTCCGCATCCGCTCCACTAACCGATCAGATGCTATTGAAGCGCGCAAGGGAGGAGGCCTTCGCCAAGCACACGGCTGGCAATTCAGCCGCGAATGGCAACGGACCCAAGCGCTCCGCCCACAGTCCGCCCATTCAGGCCAAGGTACCGCCGTCGAACATGTCCTGCCCAAGCACCCAGACGCTTAAGCCGTCGCCGCCCAGCAAGATTGTGGTGAACTCGTTCCACGGCAGCAGCATGCTGAGCACGCAGCAACAGGAGGAGAAGAGCAGACTCCTCACCCAAAACGGGCTGACCAAGTCGGCGGCCACCGCTTTGGAAACGAGTCTCTACGCCTCGCTGTACGCCAATGCGCCACAGAACAGCCAGCCGCAGTTCCATAGCCCGCGGATTTCCGGAGGAGATCACGTCGCGTCCTATGTGGGCGTCTCACTGAACAAGGGAGCCGGACACGATAACGACAGGAAGCACGAAGATGATCCGTGCGCAGGTAGGGAAATATAAGTGATTTATTCAAGAGGAATCTTCTTATGCTCACTACATTTGCAGCCATCGACGACATTAACTACTACGACGATTCAGACTCGGAGGAGGAGTATGTGGGCACGCCATTCTTTCTGGAGGAGGACAACACGGAGACGCGCCAGAGCAAGCACACTCGATTGCTGCCGCAAAAGACACCATCGAAGGAATCTGTGCGCACCACGGACTCGGAGGCGTCTGTCTATTTCCGTCCGGAGGCCATACTCTCGCCCAGTCTCTTCCCGCACGTTCCGCCGTACCTGAACTTTACGTCCCACGCGGACAAGGGTCCTCCAATGCCGGCAGCATTGCATCGAGTGCTCAAATGGAAACTCAGCCCCGTGATGCCGAAGATTGTCAAGCGAGTGGTGCTCAATTCGGGCTTCCGCATCATCAAGAGTGGGT

 

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