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CRIMIC CR01360

Gene CG3515

CG3515

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2552439..2552460	22F4	FBti0206915	83287

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CGCATTTAATGGCGAACCCAAGGACA	GTGTCTCAAAAGCATAAGGACTGGCC

Images

Construct Design Sequences

sgRNA sequence	GTGAATGGTTAGAAGAGACACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTCCTAGGAGCCTTGACA	ACACGGCTTCGAAAGAGG	GTGTATCTTGGCCCCACCG	GGGTCCTCCGTTGCACTAAT

 

Amplicon 5'

Amplicon 3'

TCGTCCTAGGAGCCTTGACAGCCTCCTCCTGTACAGTTTCGTCCGTAAAGGAGATAGTTTCGCCGGGCTTCAGGCGTTTCACCCAATCGTAAACTCCATTCGCCATGGGCATCCAGAACACGATTCGTGAGTCGCTGCTCACGAACATGGTGGCTCTCGGATTGAAGCGCATGCATCGAACAGGACCCATATTGTTGCCCTTAAGCACTGCCACAGGAAATCCATCGGCAGCGCGCCAAATGTGAATCCTGCCCTTATCCGCTCCCGAAAGGATGAACTGCGAGTCCGGCGTGTAAGTGGCATCCAACGGCAGCCTTAACTTATTCGAATAGCCAGTAAAGGACTGTTGGTAGGTACCATCAATGGCCGAGACACTGAAGCACCAGGAGTGATCCGTGCTCAGCAGCAGGCTCTTCCCGTTGGGAGCGAACTGCAACTGTGTCCAATTGGCCTTGGCGTTCACCTGGTATACGAACTTTCGGCTCGGCTCACCGCCCATCATCCTCACATCGCGGATCTGGATGCCCTCCGTGCCCGAACTGGTGGCCAGGAACAAGCCCTCAGGATCAAAAGCGCATAGTGGCCGTCGCAGATTCTTTATGCGGTGTGTGTGAGTTTTGACCCGAATGTCCCACATGTAAACCTGATCATCCCGCCCGGCACTGATGAACACATTCTCGCCTCCAGGCTGGGAGGCCAGCCTATTGATAGTCTTTGCATGGCCACCGAAAACTCGAACGCAGTGGCGGGTATTAAGGTCCAGGCATCGGATGGTGTAGTCATGCTGTCGATGGTAAATTTAAATATGTATAAATTTAAAATTGAAATATATAAGAAATGTCATTTTATGCACCAAAACCATTTTTAAAAATAATGCCTCTTTCGAAGCCGTGT

GTGTATCTTGGCCCCACCGTTCCCCTTTAAAGTTTCTTACCTTTGAGGTGGAGTGCAGGACGCGAGTGTCCTGCTGGACGAAGCAGGCCACGTCGGGCAGGTAGTGGTGCATAGGCACCTTGCAGACCTGCACCTGTCGAGTGCTGCTGAAGATCGAGAGGGCGGAGTGGTCGCAGACCAGCAGGCGCAGTCCGTCCGAGGAGAACTCCATTGCAAGCGTGTGGTGCTTTGTCTCCGGGAACACCTTGGCCACCCGGAACTGCCGCATCGCGTCATCGTTCAGTCTGATAGACATTTCCTTTAAATATGTACTGTGAGAAAATGAGCTTCAATAAGAATGTGATGTTAAGGCCTGTCATTAAAATTATTTCAATCTGTCATTTTGAAGTAATTCTCGAGAGTATATTGTTTTAAATTTTTATTATACGAAAGCGACAGAAAATTGATCCTTTCTTAGCAAATTCAACTGATTTCCTCTTCCTCGCGGCCAAATGCCACTAACTTTATTACAACTGACTGACTTTAAAACTAAATTAAGCCGATTTGAGTTTGCCCCGGATCAAAATGATTAAATCAAGTGTTGAACCGTCTGCCACGCCCACCGGATCGCCCACTGGCACAGCTGGGGATGCATTTTAATTTCCTTTTCGCGACCAGCTCCCAGATAGCCATGTTCCCCATCATTGCGCCTCATTAGGGCCGTGGGTGGTGTGTGGTTTTGGCTGGAAATTGAAGGAAAAACTGTCTGCTGAGTTGGCAACGCAGCTCGCCCCATTCGCTCCAATCTCCACTTGTTGACCAAATGCCAGCAACTTGTTATTTAGGCCGATTTGTGTCATTAGTGCAACGGAGGACCC

 

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