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CRIMIC CR01362

Gene Or22c

CG15377

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:2057226..2057238	22C1	FBti0206916	83288

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCATTCGGCCTTAGATACTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCTGTTCGTGAATGGAGGGC	GGCCGAATGAGTGATGGAAA	GGCGAAGGAGTTGTCACTGA	CAACAAACAGCTCGGCAGAC

 

Amplicon 5'

Amplicon 3'

TCTGTTCGTGAATGGAGGGCTGGGAAGACCTAAAGGCACACTGAGTTTTGACACGTGCTCGATAAGAGAAATATGAATACAAAGTTTGTACTTTTCCGAAGCTATTTTTATTTGAGCAAACTTGTATATCGAAAACTCATGGTTAATAGAAATCAGTAATATACAGTAGCAGTCCCTTATAAACCTTCCTTACTTGAAGTTAGCAACCTTCAAGCTAATGCCAAAACACAATTAAAATAAGCTCAGAAGAAGCGGAAGAGCGGAAATGCAATTTATTGTAAGCCAATCCTGTTGGGGACTTATAAAATGGAGCATTGTCACAGTTTTAGGCACTCGACGACATGCATAAGATGCATAATTCTTTTTCAATGCCACAATGGGAGGAATGTCAGGCGAGTGGAAAATGGGAAAACGAGAGGAAAAGCAATCAGACTGCAGACCATTTGCCAAAGTGCATTTCTCTGATGTTTTCCATTTTCGCGACTACTTCAAGCCCGAGCACGCCACATAAAACGACGGTATATATACACTTATATTATATATTAGATAATCCCCTGCACTTTTTCACCACCAGCAATTTCGCTCCAATTAATGACGTCCCCCCCCCACACTTCTCACTTTCCGCACCCTCCAATTCTTCGTCTTCCGTCTCCGCAGAACACGTCGTCGTTGAGCGGCTTAACCTACATCTGCTATATCATCGCGGCCCTAACGCAGCTATTCCTCTACTGCTTCGGAGGCAATCACGTCAGCGAGAGTGTGAGTACGACTCCTGACCACACCCCGAAATCTCCCCCACTTTCCACTTTTCCGGGCTCAGCCCATTTTCATTTCCATCACTCATTCGGCC

GGCGAAGGAGTTGTCACTGAACTCGATGCGATTGGAGTGTTGAGTAAACTAACTTAACTAAATAATAACCAAGCATAGCTTACTTTTAGAAGCTTTTATGAATATTTATTAAATTCTCTTGTTTCCCATTTAAATCCTGCCATGGATTTCTCCATTATCATCTTGTGCTTTCCTGCTATTAAGAATATGCTGTGTGTGTATTTTTTCACCATTCGTGTAATTTCCATTTGACGCTAAACATTTAGCAGCCAGGCGGCCACATAAAAAATGATGTGCCATTCACAAATTGCACTTGCGATAAAATTTTAATTACACAACAAAGTGTGTAACCGCACCGCCCACCCCAGATCCACATCCACATCCACATCCAGCTCCAGATTCCGTGCCACAACCCATTCCACTGACTACCGACTCCTTCTCCTTCTCTTTACACAGAGTGCGGCTGTGGCGGACGTGCTGTACGACATGGAGTGGTACAAATGCGATGCGAGGACTAGGAAAGTGATTTTAATGATATTGCGCCGTTCGCAGCGGGCAAAAACAATTGCGGTGCCGTTTTTTACGCCCTCACTGCCAGCACTCCGATCTGTATGGGATGCCATGCGATGCGTTGATTATGATAATAAATGTTTATGTTTCTGTTTCCGTTTCCACTTCGATCATCTGCCCGGCACTCCAGATACTCAGCACAGCCGGCTCATATATCACGCTGCTAAAGACGTTCCTGTAAGCCCACGCGGCGTATGATTGATATGCCGGATTGGGCGCTGTCCTTCGCTGTTTGTTTAAATTAAGAGCTGCAACCGAACGGACCTCAAATTGCCTGTGAATAAAAGTGGACGGGTGGGCGGAGCAACAAGTGCCGCCTGTCCCGGCCGTAATTGTTTAATTTAAATATTTCGCTGATCTCCGCGTGCGGATTCCAACTAATGAAAAACACTTGCGGCTATGGCCGCATTAATTGGGTCGGGGGATGGGGAAATGCGGGGGATGGGAGCCACTGGATGGGGTTGCTCCACGGCGGAGACAGTAATGGCGTTTTTACGGCGCCATCTTGAGGTCTGCCGAGCTGTTTGTTG

 

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