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CRIMIC CR01365

Gene CG5850

CG5850

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:9962207..9962231	30F2	FBti0206917	83289

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TTTCGGTTATAAGATAAGGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCAGCACCGTCATCTTCAG	CCGAAAACAGTCCCAAGTTCG	AAAATCATGCTTAATTTACA	AACTACTTGAATCTCGGTAT

 

Amplicon 5'

Amplicon 3'

ACCAGCACCGTCATCTTCAGCGTCTCCATCCTCTCCGTCCACGTCCAAGTCACCGTCGTCAGCGTCATTATCCCCGCCCGTTGAGTTCGACCACTCGGACGCCGACTGATTGCTGCCAGTGGCACTAAACTTGAGGCTGCTTGACATGCCAAACGATACGGATAGCGAGGCGCTGAGTGACGAGGCATTCAACTGCTGGCTCACGTCCACGCCATCTTCGTCCTCGAAAGCGTCGTCAATGTGCGGGTGCTGATGTTGCTGGTAGTGATATTGTTGGTAGCGGTGTCACCTGCCTGCGCCCGCACCCAACAGCAGGGCATAATCATCGTTGGACTCGGGAATTGGCGCCGCTGCCGACGCGGCACCAGGGGGCAGGCGCAGGAAACTGCCACCGCCGCCAGCACCCGCACCCGGATCCCTTTCGCGCTCGCGAAGATGCAGTGTGTCGCGTTGACGGGTCGACGGCTGAGAGCCTGCCGCTCCCGGCAAGTGTAGTTGTTGTTGTTGGTAGTTGCTATTGCTACTGTTCCCATCGTCGGCCACGCCTGCTAAAGGAAATCGAGATTGGGAGTGAGTAGATTGACTTTCGGGCAGTGTATTCAGCTTGCCATCATACACAGGCACGATCAGGCGACTGGACGTTGCACCATACATGTTACCTGATTGTGACGAGTTGCCCGGCAGCTGCTGATCTTGCCGCTTGCTGATCAGATACTCGGACTCACTGCTGGAGCGGCGAGGACTGCGCGCCAGCGGCTGGTATGTGCTGCGTCCCTGGAAGCGACGACTCAGTGAACTGCCCACGACGCCCAGATGCTCGGTGACATCCTCCTGCATGTCGGATATGTCCATCATGGAGAGGAAGGCGCGACACCAGCTATGGTTCGGGTTGTTCCAGTACTGCGGCGAGTTGATGTGAAACGGATGGTGCGGGAAGCTGTAGATGTGAGCCACGGCCGCGATAAACATTTCGATGCAGATGAGGAAATTCTGGTTGGCGATAACAAATACATACATATTTGAATTGGTTTTTGGAAACTTTCACTTCGGTTTCATTTGTTGTGTTTGCAAATTTGGAAGTTATGCTTGATAAAAACTCTTAGTCATCAGTATTAAACGAACTTGGGACTGTTTTCGG

AAAATCATGCTTAATTTACATATTAGCTCTTTACTTATAGAAATAACTTATTGCCAGATCCATTCGGCTTTTAAATAGGTCACTCACCTGAAGCAGGGAAGCCAGATTGGTGTCTCCCACATCCGACCCAAAGATGTCCTTAATAATATTATAGTATACCAGTACGTTGAGTAGCACTCCTTGGCTACGGTGGAGTAAAATATTCTTAACCAATCTTAAATGCTTTCATGTGGTACTTACAAAAACGAGAAAAACACCACAGCCTTGATGCACAGGAATTTGGGAATAGGCTTCATTGGTTTGAGATCCTCCTTGTTTGCACGGTAAAATAGCACCAGGCAATACATGGCCACGAACTGGGATATATTGTTGACCACCACAATATAGGGGAAGGCCACGTTGCCCGCAAACTCGCCCTCGCCGTAAACGCCGCATAGCTCGCAGATGCTACGAAATTCCGATTAGAGTTGACTGTTAAACGGAAAAGCACATCCCGACACTCACACAGAGATAAAGGTGGTAATGGGGCGCACGACCGTGTACTGGAGGATGCCGTGCTTGCAGTTGTGGATGAATTCACGACCCATCACCCACGGCCGCATGCAGCAGAGCGGAAAGAAGTGAGGCACCTGCGGCTTGTACTCCATTGTGGCCTCTAGGTCCATACCGAGATTCAAGTAGTT

 

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