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CRIMIC CR01367

Gene pio

CG3541

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24588930..24588953	60D8	FBti0206918	83290

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AATGCGATTAACCTGGATGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCCGGGGTGAGTGGATGTA	CCAGGTTAATCGCATTGGCA	GACTGCTCCCCTCCTCAGT	CCTCAATGAGGCAGCTCCAA

 

Amplicon 5'

Amplicon 3'

ATCCGGGGTGAGTGGATGTAGATAGAGTGCTGGTCTTGTGACATCATCACTCCGCTCCCTTGTGTGTGTGTGTGTGTGTGTGGTGAATGAAATGTGTTGGCAGCGTCAGCAGTAACAACAAATCATTATCGCCTCTCGGCCATCAGTGTAAGCCGCATTTTGTTGTCTTGCGACGCCCAAAACACCGATCCACATGCACGGCAAAAAAATGATCTCATTTCCGATATCATCTGGGTGGTGGTAGTATGGTACTATGAAATGGGTTTCTACAAAAGATCTACTTAGTTAAGACAAAGACATAAAATAGTATACATGGAGCACTTCTTTTTGAAACGGTTATTTCAATTTAATAAACCAAATTACATTTTTGATGGCGTGCCGAAGTATATTATCCGGGCTTATAGATGACTCGACTCCGATTGCCACATATTTCTTTCTGTGTAGTGACCACCATGAGGATGTGACTCGCCTGCAAAGTTCTCAGCGATCGGCAGGAAGTGGAAAACCGTTTGTCACGGGCTATTGATATGCGCTTAATATCTCGTAATGCAGCAGCTTCTGTTGCCGAGAAAATGCGGAAACACGAGTCATGATGCCACGCTGCCATGAGTTGCACTTGGTGGCTGTCTGTTTGCCCAGCGCTCGACGGCTGGCACCGCCGAGATGCCGAGATGTGGAGTTCCTCGAATAACCAGCACCACTTGGTGCGAGGCTAACCCATTTGCAATGAGCTAAGCCAGCACGCGAACCCAAATTCGCCGCTGTTGACTTGACCCATTTGGCAGCAGAAAGTTGCCTTTGGGCCACGATATTCATTACAGAATCGCTGTGTTCGAGTGATTTAGCATAATGCTATATGTGAGCAGTGGGTGTGGAACCGGTTTCGGCGGCTTTCAGTTTATATTTTCGGTTTTGACTATTTGCCAAGGCTTAATATCCGTCTTTCGGTCTTCTCTCACTCTTGCAGCTCGCACCATGAAGACAGGCACTCGAATGGACGCTTTCCACACGGCGCTGCACTTAATCGTAAGTATTCTGCACTGGGTTCTCTTCCCCAGGCCCATCGAAATGGAAAATTGCTGGAGTCGGCCAGAACACATCAATCAGGCAGCCCGCTGAATGACAATTGCATGCCAATGCGATTAACCTGG

GACTGCTCCCCTCCTCAGTATGCTAAACGCGAATCCGAGTGGCCAAAAGTGGCACACCAGCGCCATTCTAAGCCGCTTGGCTTGGCCCCAAAGATCCGAGATTCAGGTTCGGGCTCGGGTTCAACTCTAACCCAGACTGGCGAAAGTTTGCCAGTCCGTCCAAAGGAGCCTCAGAATCGTGAGAATGCAGCGGTTCCCCTACTCGCCGGCAACACGAAATCGAAATTTCCCCTTTAATTAAAGAAGCTTACTGGCGATGGATTAGGCCAGGGACTTAATTTCGGCTTGTCGTGATTTATGTCAACGCTTTGTGCTTGAAAAGGGGTCGGGCATCCATGGTCCATGGTCGGACCTGATTGGATCGGATAGAGTAGCATATATACCAACTATGCTAATGAGTGACTTGTCTCCACTTTCTTTGCAGACAATCGCAGCTCTGACGACGCACGCGGCGCAGATTCCAACGGCAATGAGTGAGTACATAAATCTGTTCATTAGGAAGCTAAACCGAATTCTATGGATTGGGACACCGAGCCACTGGGGGCCCAAAGAAAAATTGCCAGCCAATTGGTAACGCAGACAGACGCTGGCAAGATTAAACACAGTTGGGCGCTTTGTTAGTCGCTTGAAAAACATAAAATAATATTCAAATGAGCCATCGAAATCGAAGTTATCAACTATTGTAGTTTCCGCTCAAACAGAAGTAAACAGAAGAGAGAATATAATCAAATTGGGAATATTTAGTATAAATTTAAGGTTAAGTAGGAAGAGTATTTAAACACAAAGTACCTAGTTAGAAATCCATCATGACGTAACCAGCAGCTTACAAATATGTATATTTATAAAATGGATAGCAATGCCTGATGAATTGGTTCCCCTTCAAATTGGCGAAGCATTACCTAATTAGTTATAAAGGCACTCCACTTAATCCGCCAGCGTTTTTGCCATTGTTAAGTAAACCCGCTAAGCTGGCTGTTGTCTCTCCGGTTGCCAAATCTCTGTTGACTCTGTTTGCCAAAAACCAAAACGAGGTATTATAATCGCATGGCAAACAGCGGTCCAGTGAAGTCGCTTTGGAGGCTTTGGAGCTGCCTCATTGAGG

 

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