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CRIMIC CR01369

Gene dmpd

CG11866

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:10118622..10118648	46F1	FBti0206919	83291

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGACCTATTAGCGGAAAACGTTTCG	TGGGCAGCGTATAGAAGTCCTTGTTG

Images

Construct Design Sequences

sgRNA sequence	AGTGCAAGCGTGGTACGACTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTGAAATGGCTTGCTGGGC	GCTTGCACTTGGGCAAATTG	ACAAACTACCTGCACTTGTATGC	TGAGAATCGCCTGGACTTCG

 

Amplicon 5'

Amplicon 3'

TTTGAAATGGCTTGCTGGGCGCAATTGGAACAGCAATGTTTCAGTGGAATCAGCCTATAATAATCGATGTTTCTGCAGCAACTTCACTGCTTGCAAGCTATCGGCGCAATCGATTGTCGGTGCCATCGATGCGCTTCCAGCCCTAGACACTTTGACTTTTTTTGCCACGGATTGTGTATGTAGTTTTAATTGTGCTCGAAAAACTGCGTTAAAGTGGATGTCAGCGTGGATGAACTGCGTCTCAAAGAACTAGACATATTGGACCACGGCGAAGCGCGTCCAAAAACAATGCAATCGCAATAGTTCCATTTACTAAAACGGGTACGAAGCCATCTCGAAATCGAGTCGCAAAGTGCGTAGCACAAAATTTCAAAGAAATTTCGAAAGGGGGGAGAAAAAGCAGCATAGAGACGCTACATATATATGTATATAGTCGAATTAATGCGTATATACGTTGTTTTCTGTGTGTGTTCCGCTATTGCAGCAACTTCAAAACGCTGCTGCAAACGAATTCTGTCCATTTAGCACCGAAACGCCACTGGAAGCAGAAGGAAATGGTGTCCACCCGCCAAATGTGCAGTGGAGGCGGTACGTCCGCTTCGGAGGGGGTTTCGCCGTTCGGGGGCGCGGGTTCGGGATCGGCCTCTGGAACGGGAATGGCTTCCGGATCAGCAGTGTCATCCGGATCGGGTACTGGAGTACAAGAGCCCCCCGAGTCCACAGCAAACCGTTCCTCCGTCCTGCGGCGAACCACCAGCTATCAGGGTAACCAGGCCCACAGTTCTTATCGTGTCGTCTCGGCGGGCAGCCTGGCCGAGTCCGCCAGTGCGGTGACTCGCCTCCACTCGTACACCATGCAGATGCAACGGCAGCGTCCACCGACGGCCACCACCACCACCACCACCAGCGCACCCAGTCTGTACGATCTTCCCAACGAGCTGATTGAGAAGATCCTCAGCTACGTGGACTACAAGAAAGTTTCAAATCTCAGATTGGTAAGTGCACTGCTGCCCCTCTGTTCCACCCAAAGCGCTGGCTATTAAATCAATAAAAAGCAATTTGCCCAAGTGCAAGC

ACAAACTACCTGCACTTGTATGCCTCTTCTGATATGTATAAGGTGTATTCTGTCGCTCTGCTGTTATGAGCATTATTTTGCGCCATGCGTTGCACTTTCCTCAACGGATTTCGGATTTCCCTTGGCAGGAGGCAATTAAAACTTTGTCACGAGGGCACATGCATTTGCGAATGCTTATTTTTAGCAAAGAACTTGGAATCTTGGCTTCGGTTGGGGGTATGAGCAAATGTATTACATTTTTCAGATTTAGCGGCTTTAAAAACACTAGGTAATGGTTGAGTTCATTCACTTTTTTTTTTAATAAACAACTAGCTTTACACAGAAAGCATTAAAGTGAAAAAAGAACAAGCCAGTTGAAGGATTATACACATTCAAATAAAGAGATAAACATTTATGAATATCTAATATTCTACTTTTCAAACAAATTACACCAATTATAGAAATATCACCATTTTAAAGTCACGATTATCGCATACAGACTGCGTAAGAAACAAATCCAAGAACACTTTCACAGAACAGCTTTAGTTCAAGGTTCAGAGGGTAACTACACTTCGTTGTTTGTTTCTCGTTGGAATTTTTTGCCTGCACCCGCGTCTCCCATCCACAAATCGTTGTGTATGTATGCACGGTTATTTGTCGTAGGTAGCGGAGACAAATGTAATCTGATATAATTTAATAACAGGCAGAACCACTAGCTCGTGGATGAATGCGGGCACAGCAGCCGAAAAAAGAAGTCGGAATCTGAGCTTAGCTTAGCCCAAAGCACGACACATTTTTATCTGTTTTACTTACCCCCCTATTAACTTGATTCCCGGATATGCATACTCGCAGGTTTCGCACCGCATGAACGACATTTGCATGGCCATGCTGAACACAGCCTTCACCAAGCAGATCAAGACCACATTGAGCCGCTTCCAGGCGATCAAGGCCAGCATGCCGCGCCGGGAGTCCCATCGTCGTAATCATCCGTTGGCCTGCGAGTGCGACATCATCGAGACATGCTACATGCGTCTATCCCTGCTCCAGATGTCCATGGGCAAGCACATCGAGCGGGGCCATTGTTGCTTCTTTCCGGGCGCCGTAAGTGCAGTGAATAGCATACCCGAACTCGACAAGCGCTAATAAGATGGTTGTTTATTTCCAGATACTAGACGAAGTCCAGGCGATTCTCA

 

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