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CRIMIC CR01370

Gene JhI-26

CG3767

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:16289742..16289757	53B1	FBti0206920	83292

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGAGATCCTTCTTATCCCGCTTCAC	ATGGATAAGCTGTACGGCAGGAAACG

Images

Construct Design Sequences

sgRNA sequence	AGTTTGCTGGAAAACAAAACTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTCGCTTGCATTGGACAGA	AGTAGTACGCAGTTAAGGCACT	TCCAGCAAACTCAGATAGCTT	CCTTGGCGTGCTCCCTATAG

 

Amplicon 5'

Amplicon 3'

ACTCGCTTGCATTGGACAGACGACATCGTCGTGTTGAGCATTCTTCGACATTTTCAACTGATCGTGTCGGCCGCTCGTTATTTTGCCTTTTCGCGAAGAAGAGGACATATATGACATGTTATCCAAATCCGTGATCCTGATCGAAGCACTTGTCCTCTTTGGCACTTTGGATGGCATGCTGATATCTCGACTTTGCTGCGATCCATTCGTGAACTTTCGAACGTAGGTGTTGGCCCTGGTCATTGACCCGGTCGTTTTGAGTAAATACCAGTTGAGGCCCATTGGTAGTTGGTCGATAGTCGAACTGCAACGGTAGGATAGCTTTTTCTAAAACTGGAAATTTTGACTAAAAATATATCGATACCTGTGATTATGGAAAATAGAACTTAATCCAAAGGAATCATTAAACTTTGCACACAATAAACAAAAAAAAAAAACTAATTAAAACTAATAAATAGCGTATCCCCCTATCTTTGCGTCATGATATCTTATCATATCATTTTAACATGCCAAATTGCATCATGCTAATCATTAGGTAGGTACTGCTTCTCTATAAACCATAAAGTGCTTTTAATGATTAGAAAGCTTTTCATTTCCAATTCGCTCTTATCGTGTAATCTCTAACAACGAATGAGCTTTTGGAGAGTAGTACAAAATGAGCTATATGAAATGGTTTATGCTCATTCGCTATCCACAAGCGAAGCGGTTGGGATCGTCAGTACCGACGAAATGGAGAAGATCGAGGCTGAGAGGCAATGGCTGAGATACACGGTTCTTCCGTCAATTCTGAGGAACGGGCGCCTGGTGGACAACTACAGCGAGTCCAAGGTGACCACATTCCATGTGGGGGACATCGACATTGATGTAATTGGCCACTCGGAGGCCTTTATGCTAACTTTTTGCTACCGGACAACCATTAATTTTGAATACGACGGGCAAAAGTTTCAGCGAAAAATGGTGGTTAAGGTAGGTTTATTCCATTCACATAGTATAAATAAATCTTAAACAATCTATCTGGATATCTAAAAACACAACGAAAAGATTTACTATACTCGCGGAATTTAAATTATATTATATAAGTGCCTTAACTGCGTACTACT

TCCAGCAAACTCAGATAGCTTAGTGAGAGTGTGGGTTTTATGGCAAACATTGTGAAATAGAGGGAGGCATCTCAACAAAAGAACAAAGCGAATTGATTTTGAATTCAAAATAAATCACATCTAATGATGAAGTTTACTTTGGCATGCAATGACCCCAGAATACCAATAATTAATTGTTTTTTTTTTTTTTTGTAGAAAACACCTGCTATGCCACCAGAAATGTATGAATCCATACAGTTTGGGCCCCTTTTTACCAACGAGATAAATTTCTATACGGAAATTCTGCCAGAGTTTCAGAAGTTCACCGATGGCAAGTTTGCCGCACCCAAGTACTATTACGGCGAGTTGAACCAGCATTCGGCTGTGGCCATTCTGGAGAATTTCGCGGAGCAGGGATGGCGGGTCACCAAGGATCGTGTGGGTCTGAGTCTGCAGCACGCCATGATTGCCGTGAGTTACCTGGGAAGGTTCCATGGCTTTGCGTACGCCATGAAGCACAAGAACCCGGAAAAGTTTGCCCAGCTGACTGACAATCTGAAGGAATCTCGCTATGCCAACGATAACATTCATCCGGAATGGAAGCTCACCATGAAAACGAGCATCGATCGAGCGGCCAAGGCGGTGGCCACCTATCAGCCACAGATCGACGAGGAGTTTGTTAAGAAGTTCTGTTTCATGATCTCGGACTATTCGCAATACGGTAGGCAACGGGTGGCGCCCAGGGAGCCACTGGCCACCCTGTGCCACGGCGATTATGTGAGGAATAATGTGGCGTACAGGTACGATGACAAGGAGGAGCCGCAGGAGATCATGATGTTCGACTACCAGACATTGCGAGTCTCCTCTCCCATGGTCGATCTCAACGTCTTTCTAGCCGTGTCCATTTTTGCCGAGGTACGCGATCCAAACTTCGAGGCCATCTTCTGTGAATACACCTTGGCGCTTCATAATAGCTATAGGGAGCACGCCAAGG

 

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