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CRIMIC CR01371

Gene CG30158

CG30158

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:6908610..6908616	42E1	FBti0210333	86346

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	ATGCTGACACGGGGATTAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATAGCACACGAGTGGGAGGA	AATCCCCGTGTCAGCATCTG	GCTTCAACCAGGAGTCACGA	CACGATAAACCACCGTCCCA

 

Amplicon 5'

Amplicon 3'

ATAGCACACGAGTGGGAGGAGCCTGCGTGGTCGCAGGCATCACAGCTGCCTCCGACGCATCCTTACGACCATGTCATGAAATGGAAAACTTCTGATGGAACATCATTCACTGCTCCGCTGGAGCTGATTATACGCTGTTCAAACTTGTCTTACACCACAAGCACATGTTCGGCGAACAAAAGTTGCTGGGAAAGTTGATTAATAGGTGGGCTGCCTGGCTGGCTGAAGCTGCTGAGCTTCATTATCGGGATTCTAGCTGGCAAAATTGATTCCTGGATGTGCTTTGGTGACACTGGCTCTCTAGGAGGCGGCGTCGTTGCCGGGCTCTTGTCTTCGATTTAATTAATTTCATTTTAGCGACCGACGGGTGCGCATCCATTACTTAAGCCCGCCCAGATTGAATTCCACACGGGAACTGTCACCCGACCCCAAAGTGATTTATTCCCGGCCACCTTGGGTGCAGTGTCCAGACGGTCAGGCGGCCAGGCGACCAGGTGGCCAAATGGCTAGGTGCATCCAAATCAGGTTGGCCTGGACCATTGGCCGCCGGGGCTTGCTATCCAAGCTCGAGTAGCGCCCACAGCCCGCTGAGACTGATTGCCGGAGCAGCATGTGGCCATCAAACGGGAACATTCTATTGCACTAAACGATTTTTGGTCTTTAAAAGCAATTTCGTGTATGTATGTACGTGAATGTGCAAGTGCGCACAGCAGGACGCATTTGATGCCTTAAACATGGATGGTCGAACAAAGGCGGGCCGGCGAACAAAAGCCGAGCGACCGGGAATGGTGTCAAAAATATAAATTGTGTCATTCTGCAAAGTGTTTTAATAAAGTTTACTCATTTCGGCCAAACAATGCGTTACAAATTTGCGTCACAATGGCCCAGGCTGTCAATTGGTGGGCTAAGGACCAAGAGGAGATCACGTCGACAGGGCTAAATGGTTAGATTCGCTGGCTCTGGCTCTTTGGCTTTCTGGCCTCTGCGTGCCATTGACATTCCGGATCGCGTTCTATGCGTATCGCGTACTCGACGCGGCATTATACTCGTAACCAACATAACAGATGCTGACACGGGGATT

GCTTCAACCAGGAGTCACGACCCCCGGGTCACCGTCCAGATGGCCAGACAACCGGACACCGCCGATTGGGCGGGGCATTCGTCCTTGTGCTTTCCACGGACATATCGTTTAAAAAGCACTCCATTAAATGTCCTGCGTACAAAGGGCAGTGCCAACAGCAGTGAAAACGACGCCCAAAATAATGGATAAAAAGGGATCCGGACCATGTTTTCCTATATTTAAAGTACCAGAATTTAATGTCGGATTCACTTAAAATGTATATTCCTCGCTCTGGCCTCACTGAAAACAATAGCTATGTTTATTTCCATCTGAATTTGTATCTCCAAAGATATACCAAACTTTAGCTTTTAAGCCCTATTTCCATGCCATTAAAACAGTTGAAAAAACTATACCATTTGAGCACTTTGTCGCCACTAACAAAGTTAATAAACGTTGCGCGGCAATATATTTATACACCTAAAAGAAACTAGGACGGAATACTTTAGGGCAAGTCGAAGTTTATACACCCTTACCGCTTCAACTTTTTCCTATCACCATTATAATATAGGAGTACTCCTTTTTTTTTCAAAAGGTTTTCTATCAGTATTCCATTAATATTTAAATCTTTACTAAGGCAGCTATATGATGTAGTAAGCAGAAATCCAGCAGATAGCTTTTATACTGAGCGACTACTTTAAAATGAATAAAACTACAATATAATTCCCTCTTCAAGAGTATATAACCATACTTGGCAAATTTCCGATAATAACTTTTCGTCGGTCTTTTTTAGCTGCAGCAACCTTTTGTAACTGACACCTTTGGACCAAAAGACTTTCACATTGTTAAAATCAAGCGTTGGAAAATGGGAACTTTTAGTCTCTTAAATTTGATTTAGGTTTGGTCATCATAGTAAAGAAGTGACAAGAATTTTAGCATCAATAAAAATAATTCTAAATTATTAAAAAAAAACAAAATTTGTACTGCCTACTTTCTATAAGATACATGGTCTAAAGGAGAGCACAGCAAGTAATCAAATAGATTCGTTTAAATATTAATTTTGTTCAGCTCCAACATCTCCCAACATCACAGTGCTGCCTTTAATCAAAGGACCTGCAAAATAAAATTGGGACGGTGGTTTATCGTG

 

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