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CRIMIC CR01372

Gene Cpr49Ae

CG8505

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:12402996..12402997	49A2	FBti0210334	86347

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CCAAGAGTCAATCTCGAACTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACCTCGTCACCTACCAACC	TCGAGATTGACTCTTGGGCA	ACTGGGGTGAGCTCAAAGTG	TCCATTGGCCGTCTCATAGC

 

Amplicon 5'

Amplicon 3'

CACCTCGTCACCTACCAACCCACCCACCAATCTAGGCACCCATACCCATCCCCCTACTAGCCAAGTTGCGAGTTGGTTGTTAGTTGTTCGTTGTTGGTTTTGGCCGTACTCGTGTCGCAGCTTTGCATCTTGGCTATAACGTGTGTGTGTGTGTGTGCGACGCTTTGCCAACTGAAACTGAATTGTTTGCAGCCTCGCCAGTGCAATTTTCGTATAAATAGACTCACCTGCCGGCATTGGGCATCATTAAATAGAAACGTTCGTTCAATTAACAACTGCAGTGTGCTGAGGACCCAGACGAAAGGATACACTTGCCTTTTGGATTACACTACCGAATTTCTAACAAGATGAAGAACGTAAGTGGGTGCCAAAGGAGTCGAGTGGATCGGTGGTGTTATGTAACATACGAATAGGAAAAGTGTCCATAAGGATAAAATCTTTGAGCTGACATTAGGGATACATTGTGAGAGCCTTTGTGAATCAAGAATCACTTATTTCAGCAAAGCTAAGTTTGAAAGAAATCACGTTTCTCGCAAGTTAGATGAGTTCTAAAAAAAATGACAGATACAAAATTCGGTGGCAATCCACATCTTCTTCCACCAATTCCCATAAAACATATGACCAGACATGCTCCGGCAGTAAAAGCCATCGTAAATCAATCGCCTAAGGTGAGCTTTTGAGGCATTTATTATGGTTCAGGAAAGAAACCGAGGCCCTTTTGAGATGTTTTTTTTTTTTTTGGCTAAATCTTCCGCAATTATGAGGCTCGCCAGTCGTAAAGGCGGGTGCAAAAACATATAGCACATTGCCATTTGAATGGCTTAAAAGCCAAAGGCAATTAACGGATTCGAATTCCGAGGCAGCCTGCTAGCCACGCAAAGGTCAGAGTCAGACCCACGGACCAACAACACCTGGCAGCTGTCCATCGGACTGAAAAGTTCAGACACGAAATTGGAATCCCAGATGGAAAACGAAACCCATTCAGCTCGCTTCAGCGACAATTGCCCAAGAGTCAATCTCGA

ACTGGGGTGAGCTCAAAGTGGCCAAAAAGTGAGAAGTAAGCCCAACTTACCAACTCCGATGAGGCAGTGGAACACGCCCCTTCATTTCCAAGCTCTTTTGTGTAAAAGTTAATTGAGTCAGTCGACATTAGGTGTGTGAGTGAGTGTGTGTGTGTGGAAATGTGGAAAATATTTAATTGGCATCTATTTGGCGACCTCAAAACGGTGTCTAAAAAGCCCGGTATGTGAGAACAGCAAATAAAATAGCGAAGATAGCTAAGGCAATAAATCTCCCGGCATTCGGGGACGTTGCATAATAGCACTCATAGCATCGGGCACGATTTCAAGATGTTTATTAGCTCGGCTAACATTGCGGAACTCTCCATTTCGTTTTGCCCTCGACAGTTTGCTCTCTGTCTGCTGGCCACCGCCTTGATGAGCTGCTGCCAGGCTGCCCCCCAAAAGGCGGAGGAGCCCATCGCCATCATCAGCCAGGAGTCCAACATTGAGCCAGATGGATCCTACAACTATGCGTAAGTGATCTGAATAGTTATGTTATATTTAAGGGTGAGATTAGCATAGTATTAATTGATTTACTTAGAGATCTTAGTAAGATATACACACTAATCGTTTTAATATATGAACCGTCATAATAATACGTGTTAATCAGGTATTTACACGTTAATTGAACTTCAATCTTTAATTATCTCATGTCTCTAAAACCCATTATAGTTAAAAGCCGTGCTTGAAAAATCTTAGTAATATAGCAATTAATATCTGTCCGTTGCTAAAAAAAACTGATTTTGAAGCATACTTTGGGGATAAAATATCTAAATTTCGCTTTTTAAGAAACCCCCTAAAGTGACTTACATAGTTTAAGACAAACGATAATCCCCCAACAACACTTTTCCCTCAATCCGTTAGCAAAACCAGCTATTGGTGCTTCCATTAGAATAAAAAAAAAAATATGCTAATAAAGCTAACGAAATATGGATTAGTGAGCCATATTTAAATAGAGGTCTGCGCAGAAATGCTAAAAGTTAAACAGTTGATTGAATTTTAATTCGATTTGCTTGGCGCCAGACGAATGATGCATAAAAACAAGGTGATATTAAGCCTTTTATTATATATATTTATAGCAAGATTTCAAGGAGAATTCGATTAGACCCTACTAATTTATATGCTTGCAGCTATGAGACGGCCAATGGA

 

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