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CRIMIC CR01373

Gene CG13575

CG13575

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24256197..24256206	60C4	FBti0210335	86348

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTAGTCGAGGCGTCTTGAGTAGTTCT	GGTTAGTTAGCTGCCAACACCCATTC

Images

Construct Design Sequences

sgRNA sequence	GATTAAGGGGATTTGGACGGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCCGAACTTATGCAACCGA	TCCCCTTAATCGCTGTCAAC	GGGGGATGTCAAGTTGTTGA	AGTCACCGCACTGCTTGTAA

 

Amplicon 5'

Amplicon 3'

AGCCGAACTTATGCAACCGAAAGGAGACAATAAACAAATGTGCTATAAAGGACGAAAGTGTCGCAAATAAATGTGAGCCACTGCTAAAGAAGACCGATTTGCGCAAGGTGTTTTACTCGGCGGCGGCGGCAAGAAGCGTTTGGGGCACAAACGACGGATGAGTGATTGCCACTCGTACCATCATCATGACGATGGCGTGTGGAACGACGTGCGCATAGTAATGACAGCGGATGGCAATCTGAACGATAAGCGGCACCCGATAATGAAACATTAAACTAAACCGTCGGTAGTCGCTGGCCATAAATCTGTCGGCAGTCTGCTCGCAGAAGCTTTTAGGTGCGGACCCCTTGCGCGGCATACTTTCGGGCTTCCGTTCCGTTCCGTTCGCCAATGGCCCTGCTCCACTACACCTTCGACCAGCTCGAGTTGTACCTAGAATGGGCGTTCGCCCAGCACGGAGAGGCCACGCCGATTCCCTCGATCCAGCCGTATCCGGGCGTGTTCGTGGGCGATCTCAGCCAGCTGAACCGCTTCAAGCGACATGCCTTCTCCGCCGTCGTGGGCACGCTCTTCGTCCTCGCCTTCTGCGGCAACCTGAGCACCCTCTATGTGAACAGCCGCCGGAAGCTGCGGCCCTTCTTCCGGGCCTGCCTCATCTCACTGGCCTGCAGTGACCTTGTCTCCAGCATATTCTGCACCGTATCCTACATGGCCCAGTTCCAGGCGCAGTATCTGCAGCTCTGGGTGAGGCGGCAGGGATTGAGCAGACTCAACACAAGCTAATTACAAATCTCCTTAGACAATTGGTGGCTTCATGTGCAAGTTTGTGCCGTTCATAACCACCACGTCGGTGTTGTCCGGCAGCCTGACCCTGGTGGCCATCGCCTTGGACCGCTACTTGGCCGTGATGCGACCCGTGCTGGGGTTCTGGAGTCCCGACAAACGCTTCAGCACTCTGAGCATGCTGCTCATCTGGGCATGTTCGATAGGATCCTCTGGCCCGCTGCTGGGCATCTACGACTACCGAAAGATCTATCTCCTGGATGTGGAGGACTCCAGCGAGGAGAGCGAGGAGGTCGTGACCGCTGTGCCCGAGGAGCTGGTGGTCACCGAGCTGGAAATGGTTCACATGTGCCTGGCCGGGGACGTAAGTCAACGACTGGATTATCGCTTAATACAGTTGACAGCGATTAAGGGGA

GGGGGATGTCAAGTTGTTGAATCAATGTTCCGATTATCTTATCAGCCGCGTCGAAAACAATTTATGCAATTCTATAAATATTCCGTTTTAGCACGACGTCGGACTCTACTACGTCATACTGTTCACCCTGATCTTCCTGCCGTGCATTGTGTCCTTCCTATGGCTGAATGCCGTGATTGCGAGGCAGCTCTGGCTGCGGAGACACTACCACCAGGAGCAGCAGGAGCAGCATCAGGAGCCCAAGGAGGGTCAGTTTAAGACCATGGCGAATGGCGGGGATCTCTTGATGCCCTCCACCTTGGTCAGTGCCATGGGAGTGGCCGTTCCCTTTGCGCTGGACAACACTCCCTTGCCGCCCAAGAGTACCGTCAACGCTCCTGGCAAGAAGACCACTGCTGCTGCTCTAGCCAGGGAGGCCAGGCACCGCAAGATGGTGGTGGTGGTGCTGCTAATGATGGCGGTTTTCATATGCCTTCGACTGCCCGCCTGGGTCTTCCTGATCATGCGGCTTTATGGATCCTACTCCGAGCCCATCGACTGGCTCCTGTACTTCAGCTTCGGCATACTGAACCTTTTCAGTTGCGCCCTAAATCCCATCTTCTACACCTTCCTCACCCAGACCATTAGGACCTTGACGCTGGTGAAACACAAAATTCAGGGATTTCTTGGCTGTCCGCCTGGTAAAGTGCCGGATGGTATGCCCACGGATCAGATGGACAAAAGCGGGTGCTGCTGCGGCCTGAGGCCACCCACCTTCACATGGAGGTGCCATCCCAGCCGCGATAGGGCAGCAGCCACCGTAATCCGGGATGTGGACCAGCCAGATCCGCCCTCCGACCAGGTGCAACCGGATCCGAGTAGCCTACGGCGGTTCCTCAGCTACAAGCAGGAGGTGTTCACCATTTACAAGCAGTGCGGTGACT

 

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