CR01375

CRIMIC CR01375

Gene Nplp1

CG3441

Information

Donor Location Cytology FBti Stock number
pM37-p02R:25033385..2503340760F1FBti021033686349

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence GTTATCATAAAAGGCTCTCTGGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GAAGTGGGTGCTGCAAAAGG CCAAGTTCCGCGGAAATGAA TGATAACTGGCTGCAGGAAAG GGAACAAGGAGTCAGGAGCC
 
Amplicon 5' Amplicon 3'
GAAGTGGGTGCTGCAAAAGGGCAGTTCAATTTGTGAGGCAGTGCGAGTGTGGTCGATCCTTTTGTCGCGTTGCAAACGAACGAAACTCACTTAACAGTTGAGCCGCCCCCAGGACGACATCCACGATGCAGGCGGTGCTCCAATCTGCGCACAGCAGCAGGCGGCTAATGCTGTTGCTCTCCATGCTGCTCAATGCCGTAAGTCCTGGGGATTATATTTTCAATTTTAAAAACTGTAGCGCTATCAACATTTTTCGGTCTTTGGGTTTCGTGGACTGTGCTTCTGCCTTCTACTAAATTTATCTTTTATGTGTTGACGTGTGCGGTGGGCCGTGAATTTTGTCGAAATTCGTTTGAGGACTGCGTGTTTTCTCGACGAACGAAAATTGAAAACGCGATATTATCCTTAGAAATCTTTCATATTTATAAAAGCTAATTAAAGAGCTGACATGCTTCTTCTTCTTCTTGCATTTCTAACCCAAAGTTTAGCATGATTACATCTCACCATTGTTCAACGAAGTCTCCGCTTTTCATAACTTGTGCTGTCAGAATACGCAAACTTGTCTACAGCTTCTAAGCTCCAGTGGCTACAAAACGAACAAATTATTTCCATTTTTCAAGGACCCCGTTCACCACGCAGTAATTTATATTGGTACAGCCACGCCCATTCAGGCATAACTTTCCGCGGGTTGTTTTACTTTTGCAAGAGATACATCTGCGGTTGCAACCTCATCCTTCTGGTCAGAACGAGCACAACACGTTTACAGTGCAGTTATTCATATGGCATTCTCACTTTTCCTTTTGGTACATTCAACCATCTCTTGCGGAATAGCTGGCATTCCTTTTGTCCCTCACTGACATATGTATGTGACATTTCTTCATTTTTCATTTCCGCGGAACTTGG TGATAACTGGCTGCAGGAAAGCCACCTTTAACTACATACCTAATCAAATTGTTTTTCTAGCTATAACAAAGAAAGAACTTTCATGTCGAGCATCTCTCCTCTGTTGTCTTTTACCAAACACAAATAAAATTACATAAAATATTATTGTTGACAGTTTTTGCACGGAAAAAGCCACGGGCTATGAAAGGCTTTGACGGTCAGGAGCGGAACTTTTCATTTTAGATAAAGTTAACAGTTTAGTTCCTCAAATACTTAAATATCCTTACGTCAGTAAATCCAGCAACGTCAGAGGAAACTGCAACGTCAATGACAACTATTGTACAACATGGTTCAATGTAAACTTGCTTATGATTTCGAAGTTGCATCGCTGCATTACAGACAATGAATCAGCACTTTAATGCATATCGATGCAAACAGTTGACGTTAAATGTATGACGACAATATGAAATGACAGAAACGCGTGAGTGTGCCTTAATCTGGCATCAGACTAGATATGTGCGATTTATGATATGACTCGTCATTGTCATATGCGCAACCCAATTTAACAAACAAGAAAATCAATAAAAATAAAAATTAATCTTTTCCATGGATATCGTATACGCACGGAAAAAGGCGAATAAATATTCAAATTTTTAGTACCTTTCACTTTTTTCAGACATTTATTTACAATATTGACATGGGCTCACTTGGGAGTTAGCAAGGATTAAATTTTGTCTGAAAAGTTTCTGATTGGCTTTTGATTAGCTGCCTTTGTATATTTATTTTCGTGGTCAGTAATTGCCAAATGCCCTCGTAACCATGATATGTACACTAAAGCTCTTCCACAGAGCTTGCCTACAAAGTGATTTGGAAAAAGTTACATGTGTGTTTCATTGCAATGACCATTATTGTTAATTGAGCCATTTCCACGCAGTGGACACAAATAAAACTGAATGGAAACCAGATGTAGGGCCAATAAAATTTCTGTAGTGTGGATACAAAAAAACGCAGAGGTACTAGAACGAAAAAGATAATAATACAATTTGGGATCAACATTAAATATCGTTTATAATATCTTCCTATCATAAAAGAGAGTATTTTCAACAGAAACATTTTTATCGTATAAAATGCGGAATGCAGTACCTTTTCCCTACTTACAAATTTCCCTTCGTAAATTACATACGCGCGTGGCTCCTGACTCCTTGTTCC
 

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