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CRIMIC CR01375

Gene Nplp1

CG3441

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:25033385..25033407	60F1	FBti0210336	86349

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AAGCTTTGTAGCTGTGCACTGTGCTG	TCTTGCTCCCTGGTTTCCCCACATTT

Images

Construct Design Sequences

sgRNA sequence	GTTATCATAAAAGGCTCTCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGTGGGTGCTGCAAAAGG	CCAAGTTCCGCGGAAATGAA	TGATAACTGGCTGCAGGAAAG	GGAACAAGGAGTCAGGAGCC

 

Amplicon 5'

Amplicon 3'

GAAGTGGGTGCTGCAAAAGGGCAGTTCAATTTGTGAGGCAGTGCGAGTGTGGTCGATCCTTTTGTCGCGTTGCAAACGAACGAAACTCACTTAACAGTTGAGCCGCCCCCAGGACGACATCCACGATGCAGGCGGTGCTCCAATCTGCGCACAGCAGCAGGCGGCTAATGCTGTTGCTCTCCATGCTGCTCAATGCCGTAAGTCCTGGGGATTATATTTTCAATTTTAAAAACTGTAGCGCTATCAACATTTTTCGGTCTTTGGGTTTCGTGGACTGTGCTTCTGCCTTCTACTAAATTTATCTTTTATGTGTTGACGTGTGCGGTGGGCCGTGAATTTTGTCGAAATTCGTTTGAGGACTGCGTGTTTTCTCGACGAACGAAAATTGAAAACGCGATATTATCCTTAGAAATCTTTCATATTTATAAAAGCTAATTAAAGAGCTGACATGCTTCTTCTTCTTCTTGCATTTCTAACCCAAAGTTTAGCATGATTACATCTCACCATTGTTCAACGAAGTCTCCGCTTTTCATAACTTGTGCTGTCAGAATACGCAAACTTGTCTACAGCTTCTAAGCTCCAGTGGCTACAAAACGAACAAATTATTTCCATTTTTCAAGGACCCCGTTCACCACGCAGTAATTTATATTGGTACAGCCACGCCCATTCAGGCATAACTTTCCGCGGGTTGTTTTACTTTTGCAAGAGATACATCTGCGGTTGCAACCTCATCCTTCTGGTCAGAACGAGCACAACACGTTTACAGTGCAGTTATTCATATGGCATTCTCACTTTTCCTTTTGGTACATTCAACCATCTCTTGCGGAATAGCTGGCATTCCTTTTGTCCCTCACTGACATATGTATGTGACATTTCTTCATTTTTCATTTCCGCGGAACTTGG

TGATAACTGGCTGCAGGAAAGCCACCTTTAACTACATACCTAATCAAATTGTTTTTCTAGCTATAACAAAGAAAGAACTTTCATGTCGAGCATCTCTCCTCTGTTGTCTTTTACCAAACACAAATAAAATTACATAAAATATTATTGTTGACAGTTTTTGCACGGAAAAAGCCACGGGCTATGAAAGGCTTTGACGGTCAGGAGCGGAACTTTTCATTTTAGATAAAGTTAACAGTTTAGTTCCTCAAATACTTAAATATCCTTACGTCAGTAAATCCAGCAACGTCAGAGGAAACTGCAACGTCAATGACAACTATTGTACAACATGGTTCAATGTAAACTTGCTTATGATTTCGAAGTTGCATCGCTGCATTACAGACAATGAATCAGCACTTTAATGCATATCGATGCAAACAGTTGACGTTAAATGTATGACGACAATATGAAATGACAGAAACGCGTGAGTGTGCCTTAATCTGGCATCAGACTAGATATGTGCGATTTATGATATGACTCGTCATTGTCATATGCGCAACCCAATTTAACAAACAAGAAAATCAATAAAAATAAAAATTAATCTTTTCCATGGATATCGTATACGCACGGAAAAAGGCGAATAAATATTCAAATTTTTAGTACCTTTCACTTTTTTCAGACATTTATTTACAATATTGACATGGGCTCACTTGGGAGTTAGCAAGGATTAAATTTTGTCTGAAAAGTTTCTGATTGGCTTTTGATTAGCTGCCTTTGTATATTTATTTTCGTGGTCAGTAATTGCCAAATGCCCTCGTAACCATGATATGTACACTAAAGCTCTTCCACAGAGCTTGCCTACAAAGTGATTTGGAAAAAGTTACATGTGTGTTTCATTGCAATGACCATTATTGTTAATTGAGCCATTTCCACGCAGTGGACACAAATAAAACTGAATGGAAACCAGATGTAGGGCCAATAAAATTTCTGTAGTGTGGATACAAAAAAACGCAGAGGTACTAGAACGAAAAAGATAATAATACAATTTGGGATCAACATTAAATATCGTTTATAATATCTTCCTATCATAAAAGAGAGTATTTTCAACAGAAACATTTTTATCGTATAAAATGCGGAATGCAGTACCTTTTCCCTACTTACAAATTTCCCTTCGTAAATTACATACGCGCGTGGCTCCTGACTCCTTGTTCC

 

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