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CRIMIC CR01376

Gene CG13744

CG13744

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:9016052..9016086	45A1	FBti0210337	86350

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK

Images

Construct Design Sequences

sgRNA sequence	TAATGTCTATATGTACATTTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCTGTTTGCTCTCGTGCCA	AAATGGCATTGACTCATGTTAC	ACATATGAAAGTCGTACACCG	CCTCGAATAATCCGACGCCA

 

Amplicon 5'

Amplicon 3'

ATCTGTTTGCTCTCGTGCCAGCGAATGCAATCCAGTGTGGCTGAAATACAAGGTATATATTATAACATAATTTAATAATAGTAGGATTCAGCACTCACTTATGATGGGAACACTGGCGACTTGCAGCATGTTGGTGCCCGCGTGGCCCATGTGCGCCTCCGTCTTGCCCCATCCTGCAATGAGGCCCTTGCGCCCAATCAGCCGGATCGGATACTGCGGCAGGCAGATGGGCAGGATGTGCTCCGTAAAGGACGTCGGCTGGGCCAGCTTCAGCAGGGCTATGTCATACCTATCCGGCTGGGTCATGCGGAAGTTGAAGCGCGGATGGATGATCTTCTGTAGCACTCCGTGCTTCTCCACGGGCAGCGGTTCGTGTATGTGACCCAGATCCTGCGTGTCCAGCTCTCCCAGGTACACGGTTATGTCCGCCAGGTGGGCCTGCTGGATGCAGTGGGCGGCAGTGGCCACCATGTTGGCCGAGATGAGCACTCCACCGCATTGATACTCCGCGATGCGGATGTGCGCCTGCCACGGATACTCCGCAAACTGCGCTGGCCGACCGCCAATAATGCGCTTCTGAAGCGTGTTCTGCGCCGTCCGAGGAACTCCACACTCGGGCTGGGAAAATGTTAAGTATATTAAGTATGGTACATGTAACATGAGTCAATGCCATTT

ACATATGAAAGTCGTACACCGAAATGGAAAACCACTGAAAATAAGTAGCATCTCCATTCTAGCTCTAATTAGGAAAATCCTGCCCGTCTGTCAAATGAAATTGCTCAATTATCCCGCTCTGCGGTCGCAATGGCTCAATTGCCGCAGCACAGCAGACCCACCTTGGGATTGAGCAGCTCGTTGTCGTCACGTCGGCGCAGCATGATCCGCTTGGGCAGGCTGTTGAGGTTCAACTTCAGCTTTCCATAGTCCACCAGGTTGGCCAGCGGCGAGGAGGGGGAGTGATGCTGCTGCTGGTGCGGATGCTGTGTCTCGGCGACGCAGCAACTGAAGAGCCACTTGTTGCTGCCACATCCCTTTGCGTGCTTGCCGCCTTGGAGCCAGCAGGAGAAGCTCAGCTTGCAGGGCCATACGCCGCTCTGGTGGACGCATTCCGCGGGCACCCCCAGTAGGGTGTTCAGAATATCTGGGCGGGATTGGGTGACTAGACGTGGAGTTCCTAGCGCGCGTGCATATGTACTCACTGGCTTGACTGGGACTAGACTGACAGATGAGCAGGAGGAGGCACAGGCACAGCCACAATAGCAACTGCATCTTGGCGTCGGATTATTCGAGG

 

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