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CRIMIC CR01378

Gene Cpr49Aa

CG30045

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:12377024..12377039	49A1

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTTGCCACATTCAGTTAGCTGGTG	CGACCGGTGACTTGATCAATTAAGCG

Images

Construct Design Sequences

sgRNA sequence	TCGTTTGGCCAACTGGTAGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTGCCGAGGATGAGTTAT	CAAATGCCAGGCACGACTG	GCCAAACGAAACGAGCACTA	CAGCCGGCTGTTGAAACTTC

 

Amplicon 5'

Amplicon 3'

CGCTGCCGAGGATGAGTTATGAATGTCAAGCTGGTCATGTTCAACACGATCCGAGATGATCATAGTGCCTCCAACGCCCCTCGCAATACAGGTGCACAGTGGGCGATGGCTAGCTAGGATAGTTTCTATTGCAGCAAGCAATTGAAGATGCAACTGTGCTGATGATAACTTCCAGCATAACAACTTAATGTTGTGAGATATTATACTAAACTATATTTTTCTTTGCTCTAATGATAAAGTAGAGTCCCCACTGTGGCTGTCATGTAGACAACTCATTCGACAAATTGCAGCATATCGGATGGGTCGTGCACTTGTAGGTGGATTCGATTCGATTCGGTCCGGTCCGGTTCGGTTCATTTGCGCTCCATGGGAGATGGTATTGGCCTCCGACAAGAGCCACCTAAAAGAGGCTTCCGATCGGCGGGTCTTCAAAATGCTAGGCGTTCACTCTTTAATCTCCAACTGCAATAGTGTCTGTGTGCAAAAGTTCAAGCGATTTGGTATAAATATGCAAAGAGTCGCCTCTGTTGACCACAGTAACGGATCGAGACGAACTCGGAGCAGACGACCAGGACCCAGGATCACAGGATACAGCTCGGTTCGACATGCAATACACAGTAAGATGGAAGAGATCAACTTGGGTATACTCCATGCTGATATCGCATCCGCGTGCTTTCAGCTGCTCTTCATAGCAGCCCTGCTGCTCAGCTTGGCCCAGGCCAGACCACAAGTGAGAGGCCAAGCGCCGGGAGAACCCATTCCAATTATCAGACAGGAGCAGGAGGTCAACTTCGACGGCTCCTACAAGTATCTGTACGAGACGGGCAACGGCATCAACGCCGAGGAGGAGGGCTACCTCAAGAATCCAGGCACCGATAATGCCGGCCAGGTGGGTGTAGGAGAACTCCATTCACAGTCGTGCCTGGCATTTG

GCCAAACGAAACGAGCACTAACTTGCATTTGCCCTTTTACCGCTCCAAAGGTCGCACAGGGCTCCTTCTCGTACACCTCACCCGAGGGCATTCCGATTCGGATAACCTATCTGGCGGACGAGAATGGCTTCCAGCCGCAGGGCGACCACCTGCCCACGCCGCCACCCATCCCGCCTGCAATCCAGAAGGCATTGGCCTACTTGGCCACCGCTCCGCCGCCGCCACAAGAGCAGCCCGGCGGGTTCAACAACCGACGTGGTTAGGTAATCACATCGACTTCTACAATCGAATTACCTGGTTCTACTCAATTACAAAACGTGTGCGAATGAACAATAAAACTGGCCATACAACAACAAATGCAGAGTGGATTTTCAATGATTAGCAAATGATTTAGCTGTGGAATTTAATACTGACTTTAGTAGACTTAATCAAGTGGCAGATATGCAATCAAACGATGACGTCTTGCGATCTAATGACTAATGATGTATTGATTTCCAATTTACTTTTGCGTTTGGATCGATTTCTTATGCGATTGTAAATATATTGGAAATAACCCGTATTCAGATAAGATTTAACTCACTTGGGTCCAATTCCTTGACCTTACCCTCGTGAATCAAAATATGTACTGAATATTCTGATTTTATGGGCTAGGCGCGTGTGGTCGGGAAAACCTTATTCATACTTGTTCTTTAATGAGGGGGATGGTAAAACAGCCTGATTTTTCTAGATAAGATTTGCAAAGACTTAAGTTCAACTGAACCTGAAGAAAGTAAACCAAATAAATGCGTTAGAATACATGCATAACTTTTCCATAGATTTTCATTTTCGATGTATAATATTGGCCAATCTAATCATGCTTGTGATTAATATTATATTAAATGGATATCATTAAACTCATAGTAGACTTAATCGATAATAATTTCATTAGCTAGTGCGGCACTCTATTCCAAATGGAAAGATCTTTATCCATTCAGTCAGCTTTAATGATCATTACAGGGCACGCAATATTATGTTGCCTAGTTTCAGGCATTTGGCATAAATGAAGTTGAAGCGGCTTGGAATTGTATTATCTTTTCAAGAAACGTGGACAACGTCCGAATATCGTGGTAGTACCTCCAAGAAGTTTCAACAGCCGGCTG

 

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