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CRIMIC CR01379

Gene Scp1

CG15848

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:2962657..2962679	80A-80B	FBti0210338	86351

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band

Images

Construct Design Sequences

sgRNA sequence	TGCATTGGAGAAATTACAGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGAGGTGCCGTAGCTATCG	GCAATGCCTGAGCCCGTAA	TGGGATGTGTGACTCTCGAC	GACTAGCAGTGTACGGGCAA

 

Amplicon 5'

Amplicon 3'

CTGAGGTGCCGTAGCTATCGTCAAAGTTGGCATATTTACAAAGGATAAATTTAGGAGTTTCAGGCGGTTGGCCCAACACTAGTAAAATAGGCCTTTCAGTAATTGTCACAGGGAACAAAACCCATGACCTCTATTCGACAAACCGGCGAAAAACGGTGCGTGCTTAAGTGCTACCCAGTAACTAAATATGGTTTTTTTGCTTGCTGACGTTCCTTAGACCCTTGTCCAGACCAACTACAAAAGACTATTCTAACCCGTCCACGATTAAATAGGTGTCATGATTTTAACTCCGCTAGTATATGATCGGTTTCGAAAACGGCTGTGGTTACTGTTTCTGTCCAAAATTAATTTCCAAGTTCCGCGTGGACCAACTTAACTTAATTGTGCTGGGTAGGATATGGTATTTTGATATCTGAGCCCTTCATAAAACTGCTGACAACTGAAGATGGAATTTTCCTGCAGAACAGAATTGGTCACTTAAATTTCTCTATCAAGAAAGGGATGCTTCGCGGAGTAGACAATGCTTCTGTACCAAAAATACAAGCTGAAAGACTTATATTGTGAGGAAATTAAGGAATTCATGAACCATTGCAAAACACTTACTACGCGAGCCTTCATACAAGTATAGGTTGCCAATAACATTAGCATTCACGATGCATTCTCCATCTTTTATCTTTTAGTCATTTTGCATATCGAAAAGCTCTGTGCATTTGTTCTGCACAGCACTGCTAACTGAAATGACGCTTCAATACGAACAGGACATATCTGAGCCTCACGAATATGGTATGGTAAATATGTATACTAATTTTAGTTTCTTCTTTCATTAGAGTTAGTCTCAAATGAACTTCGCAGCGTACACACGGTGTATATTTTTCTTTCAGGCGTTTCTGCCAATCAATAGGTTACGGGCTCAGGCATTGC

TGGGATGTGTGACTCTCGACTAAAAACAATGAGTGCGCCTTAATAAATAGATGATACAAACACGGTGAACTGCCAAAGGAGGCTACTAAATGCTGGTGAGGTCAGCCACTTAAGGAGATCAGTGTGGTTCGTGGCGCAACGAGCCGTACAAATATAAAAAAATATCCAAACATTTTTCAAAAGTGTGGGCCAGGCAAACTTGCGCTGGGTCTACGTTTTTGGAATCTGTATGCTTAATCTCAACTTCCCAGCTTTTATGACCAGATCAACTATGTCACCCTACGAGTAATGAGTAGAAAAAGGTTGGATATGTATTTCGTTATGTCGTAATCGTTTTGTCGATTACAACGGAATATATTTAAGCTTAATGTGAATACCTTATCGTCGTCAGCAATAGCAGAGATTTCATCCCATAAATTTTTCATTAGCTTTTTATAATCATCCAATCGAGCAGTGCTGCAATCACCTTTTCCTTCTACGACACATGCTCTTACTGCCATGCACAGAAAATCATTTTGGTCCAGAAATCCATTGTTGTCGATATCTAAAAAAATATATAATTTAGTCAACGTAGTATTATTAAAATAGCTAAGTAACAGTTATCTAATAGTCGAGAAACTCGACTATAACGTTCTCTCTTGCTAAGCCTGCAATTTATAAATATTGTTCAAACAATTAGTGCGGTAAACTTTTCCAATGGAAAATAAATAATTTATGACGTATTTCCTTTGATTAAGGAGAACTTTAATATCTCGCAGATATTTCCATGGCTTTTTAGATTTGTAAAAAGTTAAAAGTTTGTTATAATTAAACACAGGAAAAGCTTCTTGCAACAAAAAGGGCCTATTTGTACTTGCACGGTGGCAGGGACAAAGGACGACAACGACAAATGGTTCTACATGGTACATTCGATTTTTGCAAGAATATTTGCCCGTACACTGCTAGTC

 

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