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CRIMIC CR01382

Gene CG3505

CG3505

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:14657929..14657941	88C10	FBti0210339	86352

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CGATTATATTGGGCCTGTTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCCAAAATTCAATTCGCGC	CAGGCCCAATATAATCGAGCT	ACTTTCACTGCCCAGCTTCA	GCACTCCTGGGAATTGGTCA

 

Amplicon 5'

Amplicon 3'

CGCCAAAATTCAATTCGCGCCGATATCACGTTATCGAACGCTGCACAGCTGGTCATACTAGCTGACCAAGGCAACTCTGCGATCTCGTCCACCTGGGCACACTAGTTGGCGGTCATCGTGTAGTCATTCAATTTCCGACTTCAAACATCCTTCATCAGCCTGCAAGCGAGTTCCGACAAATTTTCAACCCGGTCATGGTTAATTCACACCACAACTAAACGCCGAGATGCCGAACATATGAGAGGCTCGACTTAAGATGTTCGGCATTCGAATTTCTAACGTCTCCAAATGGGAGACTTTTAAAATCACGATCAGTATATCAGTATTAGCTTGTGAGCTTATTCCACAAATAAAATATTAATTACTAGATCTCATCTAAGCGTAACACGCTGAAATCTCGACAACTCATTGTAAGTGAATATGCCGTAGTGCAGGGCATTAAAAGCTCTACAAAGCCCCCCAGTTACACAACTACGATTATGTTATCTGAACCCGGTCCGAAAGCGACTCTCTGGTAATTCACAGGCACCGGCCGAACTTAATGTTAATCTCAGACTCAGCTTCGATCGCAGTCTCATTCGTTCCGCTCAGCTGTATCGTATCTGATCGATCCGGTCCGGCATGGGAAGTTTTCCAGCTCTACTTGTTGTTGTCGGCTCGCTGGCATTGGGCGCGAATGGTGAGTGTGTACGGAATACGGGATCGTAAACAAGTCTGGCTGTGAACTTAAGTTAACGTGGGACGATCCCCGATCTCCGATATTCCAGCTCAACTCCCGCCCATCAATTGTGTTGCAAAAATTCCGAGTGGCCGTGTCACCGGTCACTGCATTTCGATCCGGGAGTGTGACTACTTCATGAGAATCCTGCTATCCGGCAATCTCAGCCAGAGCGATCGCAACCTGCTCCGTGATAATCAGTGCGGAGTGCGGGGCAACGACGTCCAGGTGGGTGATTCACCACAGTATCTCTCCCTACCCAATTGAACTCGAACTTCCACAACTTGATCCATTAAGCGCTACTAAATAAAACAAGCTCGATTATATTGGGCCTG

ACTTTCACTGCCCAGCTTCATTTTTTTGGAATTTTATAAACGCTTGCGTTCTTATCATTTTCTACAGATAAGAACTTGTAAACGATTTGAATAGGGAAATACACCACATTTTGATAATACGAATGGTTTCAAATGAGATATCATTCTCCTTTTAAATATTTTAATTACTTCCATTGTTAATGTTAAGGAAACTTTTTGTAAAAATAAGTGACCTCAAACTGTATCATAAAAAAAAACTCGTTATTCGAGTACGCTTTTGGGAGTTCCTCGTAAAAGAGATATACTTTATAATATAATGATAAAGCCACTGTTTTTCTAGGTATGCTGTCCTAGTACCGCCGGCTTGGGTGCCCTGACCCACCCACTTTTGCCCTCGGATTGCGGAAAGGTTCGTTGGCAGCGTTCGAATGACACAGATACGCGAATCCGGGAGTTTCCCTGGCTGGCCCTCATTGAGTATACGCGCGGTGAGTTGCCGGTTTTGGTAATATATGTATATGTAGGTCACAGTATTAATCCCCACCTTTTTTACTCCGCAGGCAACCAGGAGAAGATCCATGCGTGTGGCGGTGTGCTTATCAGTGATCGGTATGTGCTAACTGCTGCCCATTGTGTGGCCCAGGCGGCGACTAGCAATCTGCAGATTACTGCTGTCCGGTTGGGTGAATGGGACACTAGCACGAATCCCGACTGTCAGTACCATGAGGATAGTAAAGTGGCGGATTGCGCGCCTCCCTACCAGGACATAGCCATCGAGGAGTTGCTTCCACATCCGCTCTACAATCGCACCGACAGGACGCAGATCAACGACATTGCCCTCGTCCGACTGGCAAGTCCTGCCAAGCTCAACGACTTTGTGCAACCCATTTGCCTGCCCAACAAGCAGCTGCGAGCGGACGAGCTGGAGGACCTGGTCACCGAGGTGGCCGGATGGCAGGCTAGTTCCTCGCAAAGGATGCGAAAAGGTTATGTGACCATTAGTTCGATTGAGGAGTGTCAAAGGAAGTACGCCAGCCAGCAGTTGCGCATCCAAGCCTCGAAGCTCTGTGGCCTGACCAATTCCCAGGAGTGC

 

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