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CRIMIC CR01386

Gene drl

CG17348

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19196632..19196638	37C7		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TCACATTCCAATTCAGCAGCGCCACA	TTCGTGCCCCAACTGTTGTTGTATGG

Images

Construct Design Sequences

sgRNA sequence	CTCAACGCGGGTTGCACGCAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTGGGCTTTCACAAGAACG	GCAGGGTTGAAAGTAGGAGG	ACCCGCGTTGAGTGCTTTAT	ATACGATCCCCCACTCAGCT

 

Amplicon 5'

Amplicon 3'

GCTGGGCTTTCACAAGAACGAGCTCCGATTGCGTGTGGGGATTAAATCAATGGAAAAGCCTCTTCTATTTATGCTAATCCTTGGGAAAATGCCAAAATCATCTTACCCGCGCACGGCAAGCTCAAATCAAATAACCTCTGGGCCAACCGAAAGCGAGGAAAACTCCAGCCTGAGGGCAATTAAGCCGGAGGAAAATCACTTTCCCACAATCATGATCGGTGATTAGCAGGGGGTTGTCCGTTTTAATCCTGCAAATGCATCCACTAGGCTGCAAATAAAATGTCTAAATAAACAAAGGCCAATGCACGCGACCAAAAAGCAATTGTATCTAGTGGATACCAACGCATCCAACCAACCCTCCCCTGTCCCGTACGACGAAGTGACAAAAGTCGCACACAACAATGTCAATGACGGAGGAGGGGCATTGGAAAGGTAGCAACCTTTCCAGCGGCACGACGTCGCTACCTAATGGACCAGAAATGCGTCGTGCAAACAAGCAGCCGCACCGGGAGCACCAGGAGCACCAGGAGCACCGGGAGCTCCCCCTAGAATCACAGCTCGGTGTCCCTGACCCTCGTCCTGCCGTCGAACTGCGTATGCAAACAAACGGGCGCAGGGGGAGGAGGACTTTCGCCCGGAAGCGATATGCCGGCCAGGATGGCTCTGCCACTGAAAACTGAATGAATAGCAGGCGGACCCGGCACTTGAGCACGCCGTGGCTTCCATCCCAACCCTCCTCGGAGTCAGCGTCTCGAAGCCTGTTCCACCCCTGGCGAGCAAGGGGTCCTCCTCCTCCTGCTCCTCCTCCTCCTACTTTCAACCCTGC

ACCCGCGTTGAGTGCTTTATTTGCAACCGCTGGTGGCAGGGGTGCATGTGGATGTGGATGTGGATGTGCCTGGCCAGGAGCCTTCGCCTGCAGGCGAACTGCAGGCTGACATTACCGATGCTGACAGCGGCACAGTGGTGTGCTGTCACAAAAACAAGTTATATTCACCTAAAGAAAAAATCAATATACCTGTTTATTAAGCTAGAATGCATAAATGCGTTTATGTAATGCGTAAAACTTATGGAAGACATTTTATTATGCTTTGTATTTTACCCACCCTTCGTTCCACTGTCATATGCTTGCCATATTTTTTGATTGCTGGCGAATTCCGGCGGGCGCCTCGACGTCCTCCTCCCTTTCCAACCACCTCTTCGCTTCCCACCACCCCTGGGCCATCTTGACATGACTCTCGTAATGAGCCCGTAAATTATTTTTTATTATTTTGTATTTAATTTTTTCATTGCCCGCCTTTGTTCCTTGGCACAACTCCTTTGCTTTCGGACGACCGACAGGAAGCAGAGGAAAAAATGCCGGCGCGTGTGTGGCATCCATTTGATATGAGAGAATTGATTGTGGGAATCGAATGTGGTGTTTCCGTCGCATGGAAGGAGGAGGAGGAGGAGGGGTGGTAATGAACGGCCAATCGATATTCAACGAGATGGCGAGGCACAAGTCGCCACTGAAGAAGCCTAAATGAAGCATATTATGGGTCTGTCGGGGAATCCATTAACCAAATGCTAATTACTTTCCGCACAATTATGTGCTTCGAGCTCCAAAAATCCATAAGCACATATTTACCCAGTGCAGCTTATTGGAAAATTTCACACGCCCGCACAACAGAAGCTACCATGGATGCAGTAGCTCCAGCTTCTTCCCACGCACAGGGAAGAGATAGCGAAAGTGGGGGACTTTCCCTCTACGAAAACACATCTGGCTGATTCTCTCTGTCTGTGTGCATGTGTGTGTGTGTGTTCTGATAACATGGATGGCAATTAACCATATTCTGGAGCTCTTAAGAGCCCATGAAAAGGCGATATGCATGTGGGGGCCAGTAATGATTTTCTTTTGGGGGATAATCTACAGATTGAACTAACAGCTGAGTGGGGGATCGTAT

 

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