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CRIMIC CR01388

Gene CG7781

CG7781

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:8323614..8323637	29A4	FBti0210341	86354

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GACGCGATTACGCGATTACATAACGG	TCGTCCTTAACGCTGCAGATACTGAC

Images

Construct Design Sequences

sgRNA sequence	GCCTCGCAGTCTGGCACTAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCGTAATCTTGCGGCAGTA	GAACCGCTGCCATACCCAG	AGACTGCGAGGCTCTTAGGA	CTTTCGCCGCAAATCCCAAT

 

Amplicon 5'

Amplicon 3'

TGCGTAATCTTGCGGCAGTATGTGGGTATTCCCTTGCCACGGGACGAGTACTGCTCATCGCAGTCCTTCAGCAGATTGTCCGGCGGTATGTCCAGCGCACAGTTGGCGTCTTTGTGGCTGTTGCAGACAAAGCACTTGATGGCATATCCTGAAAAAATTTAATTATTTATGATAAATTTAAGCAATATTTCAAGTAAACTATCTCAGTATCAATGATTTTATATATAAGAAAATATGCCTATCCTTTCTATTTCCCCAGTTAGTTCTTATTTACTGCACCTACAAAGCAATTCCTTAATTGTAATTGCATTTGTGCATCCTGAGTGCATATTAACATTCCATTAGTTTCCTTTTAGCTGCCACCTTTCTGTTCTTTTTTTTCTACCCCACTTGCAATCTTTCGCCTAATGGACACAAAGCCATAGTTAATGCCAATTAGAAAATTTCCATTGGCAGCAGCATATGCTTAACCTTAATAGTTTCACAAAACAAGCGATTCTGAAAGAAACAGGCGTGTAAACAAATGCGATATAAAGAGATCACTAGTGATCACTAGTGATTACTTGTGTACCGTCCAGCTAAATTGAATTTAAGGTCGTGCGCGACACGAGATTCGCATTATTTAATTTTCCAAATATATATACACATATGTACATACATATGTATATACGAGCTGGAAATGTCCGCCAGAATGGGAATGCCACCTGCTCCGCCGTACAACCTGCGATGCGCTTTTGCCTCACGCTGTGTCAAAAGTGGCACAATGGCATGGAGATGCTAGTAATGTCATCTCCACCTGGGTATGGCAGCGGTTC

AGACTGCGAGGCTCTTAGGAGTGCCTTGCTAATTGAGCCATCGACATTGCTAATGGACTCGAAATAACTTTGCCGCAACTCGCCGCCCTCGCCGAGTACTATTGTCTATTATTCTCCGTCCATTTGGGTTACATAAGCAGGGCGGAAAAGAATGTGCTGCCTACTTCGGGGCGAAGATGCTTGAGAACAATGGGAAAAGGTCCACTGTAAGATGAAACACTCCACGGGCTGGTCTAGATACTCTGAGAGCTGAAAAGCTCATAGCGACAAAGGATTGCAATATTCTATAGGAAAGTAGCGAATATGAAACGAATCTCAGATGATTCATAGACATATGAAGTGTTAAGACATATAAAGATAAAGTTTCAAAACTACTGTTTATTAAATATGGGACATATGCTCTTGCAACCCTTTTCCCAAAAAGTTTTCATGGTTCGGTTCCACTGTTTACACTTAGTCATGACCAATTTAGGTTTATTAAAGTCTATAAATAGTCGTCAATTGCATTTATTGAATTGCATAGAACGCGAAAAATATGGGTATGCATACTCAAACATAGGAAAGTTAAAATTCCTAACAGAAAGTTAAAATTTTCCTTAAAGGTTTAAAGTTTCCTTATGAAATAGTAATTAAATTTATTTTTATATAAGTCTCTGAGCGGATTTTGGTTTTGGGTATATCGGGTAACTGCAACTGGCCCCCAGGACCTTTAACCAAATAGAGTGCGCATGTCCTACATAATCCTTGCACATACACACACTCGCATACATATGTGGGAAAACTTAAATTGGGATTTGCGGCGAAAG

 

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