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CRIMIC CR01390

Gene Hakai

CG10263

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:19581936..19581940	37F2	FBti0210342	86355

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK

Images

Construct Design Sequences

sgRNA sequence	TCTTGGCTTGACCAATTCCTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATTGGCATTGTTGATCCT	CCTTGGTATCCAAACTTGA	TGGTCAAGCCAAGATGTTAATCA	CGTCAAGCTCTACCTGTCCG

 

Amplicon 5'

Amplicon 3'

GCATTGGCATTGTTGATCCTGGTCAAATTGGCCAGCGTGAGCGTCGAGTGGCCGCCGTGGATGGCGTTTTGGGCGGCGGCGGCGGATCCTGGTGGCGGTATACTACCAATACTACCGATGTTACCGACACCGCCGGTCAGTGGCAGCCGCGACAGGACGGGACGGGACGCAATGGAGGCGGAAACGGATATGGGGACAGACGACTCGGAGAGCTGCAAGGGGCAAGCGGTTGTTTTTTTATAGACAGGACAGCTTAGTTTAGGAGCCATAGCTACCACTCGCCTTGCGCTGCTTGTGCAACTCCAGGCCCAGACCGACGCCGCCCAGATCCGTCATTTTTGGCTGCTCCGCCATGGCTGACAGCTGCGGCTGTGGCTGCAACGGTGGCGGCTGCGGCGCCACATGTCTGTGATTGATGTGCGCCTGCAGGTCGCGTTGCGAAAGGTATGTTCTACGGCAGCCGGAGCTGCCGTAGCGACTGCCGCCGTGCGTGCACATGAACACGGTGCCCAGGCCACTCTGCTCCACGCGCAACACCTTGTCCGTGCAGCGGGGACAACTCTTGATGGGCTCGGCGCGGGCGCACTTGAGGCAAAATACATGCTTGCAGGGTATCATGCGGCCGTAGACGAGGATCGGCTTGTCGCACTGATCGCAGCAGTGGATCATCGGGTTCAGCACCTTCTCGCCGATCAGGCTCACCTTGTGGTTCCACTTAAGGCGCAGCATCGGCTCCGGCGGTCCGCGCGAAAGCGTGGTGAAAGTGGGCGCCTCCAGTTGCGATATGTCCGCCTCCATGTCGACTGCGCGGGCGAGAGATAGCGAGATAGCTTTGTTCAATGCTTCAAGTTTGGATACCAAGG

TGGTCAAGCCAAGATGTTAATCAATTTAACTATCTTGGACAACCATTCAATAGGGGAGGAGACTCACTTGTTTGGCTCATCACGGGCGGTGGCACTTGCTGCAGGACCTGTGGCTGTTGCGTCGCTGGAGTCATGTTCCCGCTGGCGGCGACCGCTCCTAGCGCACCGTGCGGCAGGGGCTCCTCCAGAGCGCCGGGCGTTTCCAGTTCGCCGTCCTTGTCGAGCTCCTGCATGACCGAATCTTCGGAGGCGTCCAGTCTTCCCGGACTGTCCTCCAGGGCTGCACAGCTGCTGGCCGCCAGGGCTGCGGCGTCGGCGATGCTGCTGTCGTCGATTTTCTTGCCACGACCGCGTCCTCGGCCACGTCCGCGCCCGCGAGCCCTGGTGCCACGCCCTCGTCCGCGGCCGCGCTTCACTTCCTCGGTGTCCATTGTTCACTTGTACTTGCGTGGGATTTTATGCCGGTTTTTAAGTTTTTCGGTTTTTGTTTAGCTCCGCATTTGCGTTTGGCCAGGGTTGTCAGCTGTTCCCACATACGTGCAGTAACTTAGGGTGACCAGTCTGCGCCTAACAACACTGGCCATCAAAAGGTCCGGCATCACTGGTCAGTTTAAAATACAATATTTTTACTTGTTTAATTTTAATTTTATCTGTTTTTCAATTATTTTTAAACTAATTTGAATTTAATGAATGTAAAAAAAAACATAAAAAAAAACAATAATTATAAGCATCTATTTAACCGTTCTTGTATCATATTAATAGCTTACTAATTTCTTTTGTAGCTTCAACATGAAAAAACAAAAGTATTCTGAATAGAAAATGTGAGATCTACATTAAGATAACCTTAAAAACTTAATAATAATTAATAACAAGGTTTAAAATTAGTTTTTTCGTATATAAATTTGAATTTGTAAAATGTTTTTTTAATTACAATTAAATAGTTTTTATTACAAGTTATATTTTTATACTATGCTTTAAAAAAAATGATTTTTGGTATTTGTTGCCTAAAAATATCGTTTATCCCCTTTCCTTATGCTTTAAATTGCGCTCGAATTTATGCAATAACTGAGTTGAGTAGTTCTTCAGCTAGAAATCGGGGTCATTCACCTTGGGTGCAAGGTAGTATCGAATGTGGCCGTAATCCTCGATGGGATACTCCACCAGGAGTGGCCGCTCGTCGGACAGGTAGAGCTTGACG

 

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