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CRIMIC CR01391

Gene CG9338

CG9338

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:20866143..20866153	38F3	FBti0210343	86356

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TGAAGGGCACATATTGCTGTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGGACGCCAGCATGAATCTT	GGGACAGCGACTAATCTCCG	TGTGCCCTTCATTAAAAATGCA	TGGGATAGCTCAGCGGTACT

 

Amplicon 5'

Amplicon 3'

TGGACGCCAGCATGAATCTTACAAACGCTCGCCAACTGCCGTGCAGTCAAGGTTTGCTTTAAGTAATTTTCCAAAAAAGTAGTTTCAATCCCTGAAAATGGTGTCGAGTGTAAAAATGATCTTGGCTCTCACCGTCTTGGCCACCGTAGCCTGCACTGGTATGAAAGTTACCGGAATTACCAATCCGGAGTAATCTACACATTCTACGATTTCTAGGCTACGCCATCAAGTGCTATCAGTGCGATTCCTTGACTAATTCCGAGTGTGGAAAGGACATCAAGTCAGACAGTAGCCTCGTTTTGGATTGCACCAAGATGGCTCCACCTCGATTCCTCCAGAACTTCTTCCCGGTTCGCAACGCCACCGGTTGCATGAAGCAGACCATCGACAGTAAGTAATCTGGTGGGGATCGGCCATAGCCATGATCTCATCGTATCGATTGCCCACGCGAAACCATCCCCATTTAAGCAATTTAGGTTGATAAATTAACTGGGTCTTAGTGCTCACAATGAGAATAATTGAGTTTTCACTCTTACCTGAAAATATGATTGATTCTAAAGCAACGAAATAACAAGAAATATTTGTTCAAAACAAATTAATTAATAGAATATACCTCTGACACTTCTTAATGAAAGAGAATGAGAGAATCTAGGCGTTTAGCATCGAAATATATTTAGAACTGTTCAAGATCAGCGACTTGCTATTGGAATTAAATATATATATGCATATCGTGTGTAGACCATGGAAGGAACCACATATGATGAATGGGGCATACTCATAAATATGAGAAGAAGGTTCAGATAAGGTAGACTGTACTGACTATCTTACATGTATAAAAACAATATCTATTGTATATATGTACTTCCTCATTTATTATACTATAATGATAAAGTGCGTTTGCTCACAGGCAAGTGCGTTAATCTCACAACGGAGATTAGTCGCTGTCCC

TGTGCCCTTCATTAAAAATGCATCATGACAATGGACATAAACGCAGCAGTACCTTTTTAAGTGATTTGTTCACAATTTACTTGATTGTCCAGCGATTCCCAAGCATAAATACTCCCTAGGGCTATATGGTATTGAGAATAATTGGACCCCATTCACTTTTGCTACGGAATTATTTATGTTCGGTGACCGCAACATCGCCTCAGTCAATTGTTTGCTAATTACATTAACATTCGACCACGTCTTGCGTTTTATTGAATACATCATCGTCAGTTTTAATTAAACACCAGACAATTGGCATCCCATTCAGCTCAAAAGTTCGTCACATCTATATTAGAACTTTAAATGTGTTCTACCTTTTGTGAGATATACATAGGGAATTAATAATAATTATGGATTTATTTTAATGATTACAATTTGTAATTTTTGGATTTAAATATCATTGTCCGAATGTAAAAACCTGAATAGTCTGACCAAAAAAATATTATTTATTTAAAGGAAACACACTTAAAGTCAGAATATACTTTATAGTTTGAAAAAGCTGCATACAAATAGAATTGAGTATCAGAGAAAGTCTAAAAATGAGAATTTTTGAATATTTCTCTATACATTTATCTCCGAACACATTAGAACACACAGCACGCTTTAATGTCGTAATTTCTGAATCTAATTGCCGAATATATGATTTGAATTTATAATATATCAAAAAGAATAACAACCGACAAAGCATTTCCATTTACACTTAACCAAATAATTTGTACCAACTTCTAAAATGTTTACTTGCAGTTCCCGGTAACCCGCAAATCGTGAGGTCCTGCTACTTTGGCAACATCGCCGACACGAAGGTTGGATGCCAGACCGATCCCAGCCTGACGATCAACAAGTTGCTGAGCTGCGAGGTTTGCACCGAGGACGAGTGCAACGGAACCTCCTCCCTGGCCCCCATCGCCGGAGTCATACTCCTGTTCTTTGGCCTGGCCCGTCTGCTAGCCTGAACGAGGAGCAGTTTGTAGTACCGCTGAGCTATCCCA

 

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