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CRIMIC CR01393

Gene CG13795

CG13795

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:7725860..7725875	28C2	FBti0210345	86358

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GCGTTATTGAAATCAACAGCCAGGGC	GACAGAGTTATTACACTGCCCCAACC

Images

Construct Design Sequences

sgRNA sequence	TTCGGATTTAGACTAATCTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCCATGGCCATGTCACTT	GCGGTGCTCTGCTAATGTTC	GTCTAAATCCGAACCTTATCAGC	TGCTGTCTGAGTTTTCCCCC

 

Amplicon 5'

Amplicon 3'

CTCCCATGGCCATGTCACTTGCCTTTGGAGTCACATATCTGTGGAGCCCGTTAAGAGCTCCCGGAAGGAACAGGAATCGCACAAAGCACACTAAAAGAAGAACCAGGGTAACAATCACCATGTAGCGTATAAGTTTTCCAAACTTGGCCGTCTCTGAGAATTTATAAAAAATAAATGCAATCACAACCCAAATGATTGCGAATAGGCCAACAAAATGCCAAGACAACTCGTAATCCTTATACCAATCCGGAACCGCATCCTGTTGCATGCTATCATAGTGATTTCTGTAAACATAGAGTTAAAAAGAAATTATTTTTAAACAATTCTTAAAATAATATTAAAATATTTGCATTGGATTAATTAGGTAATGTTTTATTGCTATATAAATAGGAGCACTTACTGAAAATAGAGCACGGATGGTACATGGAGCAGCTGGTGAGATCTGTTGTATTCCGAAAAGTCCTCCAAGATCAGTGTCTTATTACAAGACTTAAATATAAAAAATGTACTTAGTCTTCAATTCAATTTAGTCATACACAAATTTCATACACTAGATAGCGTTGCACTTCCATTGTGCCAAGAACTCAAGCCCTCACAGCTCCAAGGTAAAGTGGGTCGAAATGAGTTTATTATGAACAGAAGTGGAACCGCAGCAAAAATGCTATAGTAAATTAGCATGGAGATAGTTAAAAATCCACTCACATATCCCATGCCTGAAATTGATATTTCGATCAATATTTAATATCTGTTTAAACTTCTCGGTTACGTACGTACCTTTAAAGAAAGGCGACAGGCGAAAGGCGGAGATGAATCCACTGCTGGAGAACTGACCCATGAAGGAGTGAATGACCATGATGGGAACCAAGTAAATGGACATATATACAAAGTACGGCAGTATTCCAAGAACTGGTCCAAGCAAATAGTGTATTTAAATAAATGCATTCAAAGATAAAGATTAAATTCTGATGTATAAACTGTTATCTCTTGTGCTGTATAAAGAGACACAATTATTACATTAAATACATAAATATCGACAGAATTAGGCATTGTTTTGTATGTATGTCAGATGAGAAGGTAAGAGAACATTAGCAGAGCACCGC

GTCTAAATCCGAACCTTATCAGCAATATAATATACGTAGTCGCCCTTGCGTTACTTACTGCCCATGTCGAAGAACATCCAAAACGATGCCACAAAGACATCCAACTTCAAGGCTAATCCAAAGCAGGCGAACATAAAGTCCGTTGGCTTATCCCATTTTCCTCGATTCAGATCGGGGGAAAAGGGTTTATGCCCGGTATCATACGATGTTTCATATACCATGTTGAACTACCCACAATATTACCGCCAAAATGTGGGCGAGAATGGATTTAAGCAAATTTTGTAAATAAGTATATATAACTACGATCTAATTTTACACTCCCGCAGAGGTAATATTAGTATATATATATTTTTTTTCTGCAACCGATCCGCGAGCGTTTTACTCTAAATTGACGGACTAATTAGTAGAACCGATGCCGGCAAACTGAATCCGATTCCGACAGCAGGCCTCTGTTTAGCTCATCCCTCTTGAATGAGATACCCCAACTTAGGCGATGCACACTTGGTCTTTAATCACACAGTTATTACTATGGAAGTGGGGTATCTTTATACCGAGATTCATTTGAAACCTCCCTCTAATTTCAAACAGATCTGTTGTCAAAATAAAGATATGCTAGGAAAATATATATCTAAGCGAGTTATCTATAGTTTCAAGCTTTCGGTTATCAAAATAATTGTAAATTTGTTATTACACGTTTTGTAAGTGTATTTCCGATCTCGACCATAAATTTGCATTTATTGTTATTTTGCTTTAAATTCAGATTATTAGAGCTCTTATATCTGTGCCTGTCTCTCTTATGTGCGCCTATCGTCGTGCATTGCCAAGTTCATTTCGATTTTGGAACTGGAAACTGATATGGTCGTGCTCGGGAAATGGGATGACAAACAAGACAGCCGCAAAGTTATCAAATCTGGGGGAAAACTCAGACAGCA

 

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