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CRIMIC CR01394

Gene CG1888

CG1888

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:9546561..9546571	45F1	FBti0210346	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTCGGGCGAACTTCGGTTTAGAACTG	TAGGCTTTGGATTGAGCTGCCAACTG

Images

Construct Design Sequences

sgRNA sequence	AACCGCCGGCGACAAGCACAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATCTTACCCCTTCGCTCGG	CCGGCGGTTGGTTTTAAACT	CAGGGTTAAAGTCGCGTCCT	GGTCGGCATTATCGTAGGGG

 

Amplicon 5'

Amplicon 3'

GATCTTACCCCTTCGCTCGGTGCGGTAATAGCTGAGCTGTTCCTGCGGGAAAAGCTGCAGTATCTCGCGTTCCAGCAAGTGACTCCTCTGCAGGGTCAGGTGCATCTGGTTCTCCTCGTAGTAGCTGCACACTAGTTGGATGAGCAAAAGGCGCTGCACGGCGTCCAGCGGATCGTTCTGGCCAATACGCTCCAGCAAGCTCTGGGCTTTCATACCCGAGGATCGCAGAATGCCCAAAATGCTAACTTCTTGGTCAGGAGTGGCAGTTGCATCCTCCTTGTTGCCTGAGGTTCCTCCTGAAGACCCAGCCGCCAATACAGCTTCAGGCTTCCTAGTTTGCTGCATCGGCATCAGCTCCTGTTTGACCATCATTATGTCGTCGGTTATCTGCGCAGGCGGATGGACCAAAGGCAATGGAAAGGGAACTGACATTTCCCTGGGATCACTGAGGGCCACCAGCGATTCTGCTGGTATTGTCTTGACCAGATCTTCAGGCTTGTGCAATTCTTCCTGCTCTGACGGCTGTTCCATGTGCAACGGCGATTGCTCCTGACATTGCGATTGGCCCAGAATCTCCGGACAGCGACAGCCGGAGCAGTGGCTCGACCCCTGGCCCTGGGCGCCTTGGAGCGGTACGTTCAGCCAGCGGCGCCAGCGCTCCAAGTGATGCTCGAAGCGAATGCGGGTTCCAAAGCGAAAGTTTCGCAGCAACTCGGACAGATGATGACGTTTGATCATCTGCAGCTCGTCCACGTTAATGGCTTCATCTGCAACACAGTGTGGCAAAGAATAACAATTTTGGTCCGAAGAATAATAACGTTTACTATCAAGAAATTTAAGAGACTTTTAGACAGTTAACTTTAAAACTATGAGTGCATAAACAGTAGTAGTAGTAAATTGCCGTGATATTGCAATCTTTAAACAACAAAAGTGTTAACCCTTAAACACAATTTCTAGCAGTTCCAAGTTTTAACGGAAGCGAAGTATGCAGATCCCAACTCAACGAAACCAAAGTTTAAAACCAACCGCCGG

CAGGGTTAAAGTCGCGTCCTCTTTTTGCTCGCTCTTTATCAGCGTCTATGCTTCGCTTTGTTTACATATGGTACATAAGAACAGACATACATACGTACATATTTACGCATGCATGTACAGTACGCATGTATGTACGTATGTTTGCATGTACATACCTTGCAGGTGAGGAAGCAGATCGGCGACATCCCACGACTGCAGAAGGTCGCGCAGCTCCTCGTAGTCCTCCTTTCCAGCCATCTCGAGCAGTTGCAGGCCGTCAGATTCCAGGTTGCAGTTTTCCACGGATCCAGAGCCACGTGGCATCTGTGGGGGCGATGATGTACACGGATTCATTCGACTACAACCGGGCGTACATCCTTTCGTCACGAATGAGAGTGCAGCACTGAAATTTTGCAACAGGCGCGTTGTTCTCTCCCGTCCCTCACCGCACACATACCCTCATACATACGGTTTTCGTTTCGATTTCATCGCCGTCTGTTCTGTTTTTCATCGTTTTTGTTTCGTTCATTCGTAGTCGGCTTCGCCAACTCAACGAACAGCGTATGTACCAAGGGCGTAGTCAGTTATGCAGAAGGGGTTCACCATGGAAATGTGGCTTTAACTAGTCGCTATTAAACGATTGATCGTTTAGTCAATGTATTTGGTTTGGGCGTGTAATCAGTTGTAATTATTACAAATGTAATCAAGACTACGTCCATGGTATGTACGCGTATGTAGACATTCATACCTGAGCTGGCATGTGTGTAATGCAGCGTTGTACTGTTTTCATTTATTTTTGTGTGTTCTTCTGTGGCTGCACGTAAGTTTGTTTGACTGCTTATTGATAAAATTTCGCACCAACCTCGTGGTACGAACCCCTACGATAATGCCGACC

 

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