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CRIMIC CR01395

Gene CG13506

CG13506

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22390138..22390173	58D4	FBti0210347	86360

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK

Images

Construct Design Sequences

sgRNA sequence	AAGGTTATGATGGATATTGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGGCATCTAGGGCAAAGTA	AGTGGCTCAAACTCGTTATCA	GCGTTGGCCATGATGAAGTT	TCTGCGTCTATTGGCGATCC

 

Amplicon 5'

Amplicon 3'

GCGGCATCTAGGGCAAAGTAATAAAATTTATTATTGCATACCCAAGGGAATGCAGTTGCAAGTTCTAAGGAACAATTAAGATCACCAAACCTATTTTTTTAACTAAGTGGTATAGTTCTAGTTTAAGACTGACAGTTTAAATCCTTCGAACCTCTTAAAGTATTTTTTTGTCAAAGCCAAAAGTTTTATTGATATCACGATACGGCATAACAAAGTCCAACATGTTGGCCAAGTACAGAATGAAAGCCATGAGAATATTCACAGCACTTAAAGCCATCGCAAGCTAACTGGCTTTTTTCCCTAGAATTTTAAACATGACTTCATCTGCCGGCGAATGCAGAGTCATCAAAGTTGCCCTCAAGAATTGAGTGGATTGCGACAAATTCTCCAGGACGTCCCATTGCCAATGCAGGCCACAAATGGCGATTGTGTATAACATTGAACTCGAGTTTAGAGACAGAAAAGGAGTGGCAGGACGATTGGGGGAGAGTCTGGAAGAGAAGCTGGCGCCGCGACATCCTGACGCCATTGTTCCACTTAAAACGCTTGCACTGCCAGCCGATGTGAGTGCGGTGTGAGGGGGAAGGGGACGGGGTCTGGGGACATCATCATCCATACGGATGAACTTAAGCCGAGTGGCTGTTGATGTTGTTGCCGGGGAGCAGTTTTTTATTTGCCACCCCCCGCCAACCCAACACCATCATCGATCCAAGGACAATGCTCGCCCAGGGGGTTGGATGACGCACTTGGGCACTTGGGCACCCGAGCTCCCAGCTCCCAGCTTCTCGACTGACTTTTGGCGCTGACCTAGTTGCATGCAAACAATTGCGTGGAGTCCGCTCTCGGGAAGCCACTGTTTCCATGTGCCCGTAACCTTGACACCGGCCGAGGATTGCAGTTGTCAGCATTGGCCAGGGCAGCTTCCCGAGTCGCCCATCCAAACCAGATCGTTGAAGGTCCCCAGCTGCTCTGGCTGGCCAAGAGTGTGTTGTCTTTTCGTTTTCTCCTTCCATCAAAGTGGCCCAGACCATAAAAGGGGCGTCTGACTGCGGCTATAAGGAGAGGCCATTGGATAAGATAACTGGCTCCGTCTTTGGATCGAGTGTGCCCACCTTAAAGAGCGTTTATCGACCATTGATAACGAGTTTGAGCCACT

GCGTTGGCCATGATGAAGTTGGCCATGAGTCACGAGTCATTCGGCTTTTGGCCGTTAGATGACTAGTGAGTTACATGCCGCATATGGCAGGCAAATTGTTATGGCTAATACATACGAAATTGCGGTTGAACAGAAATTACTCATTCAATTTGCATAAACTCATACCTTTCGGTTTTGGATGGGGCACTATGCAAATAGAGACAGTCATGAAGATTTCAAAAAAAAATCTCTTTCGCTGAACCTGACTTTATGGCCTGCGGTAATTTACCCAGAGATTAAGATATATCTTTCATATCTAACATGTTTTTTACCTGCGAATTTAGTACTCTGCAGATGAAAATGTTCTTCGAAGATGGCCACGAATGTTAGTGGTTGTATACTTTTAGGGGGTTTCCTGTGCGGTACAGTAATATGTTAGTAATAAATATTAGTATCTGCAAATATGTATTATCAAATCTCCCCTCGTTCCTGGTAAAAACTCATTTCCGTTGCAGAACTGCACTCTCATAAACGAGTTCTTTATACTGCAACCCTTTTGATTGGAAGCAGAAGTTGCTGGGGATGTCGTGTGATTCTTATCAATTCCAATTAAGTCTAGAGTGGTTCCAGGGATCAAGTCTGATGACTCACTGCAGCACCTTGCCTATAGCAACTAAAATGACGCAATGATCTCAACAATATTCGCATCGTAAATCTCTCTTCAACAAAGTTAACTTATTATTTGAGTTATTGATGTGGGTTGTTTATGATCAATGTGGCAACCGTCTTTAAGCATCCAATAAGTAAATTTCTCTTTTTGACTTTTCAGTGAGTTGTGCAGGATCGCCAATAGACGCAGA

 

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