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CRIMIC CR01398

Gene CG45781

CG45781

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:634403..634403	?		Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
yes	yes

Images

Construct Design Sequences

sgRNA sequence	ACCCCGGTCAGCGCGGTGCTGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCCCGCAATGTCCATCTGTC	GGGGTGGGCTTGCTGTTT	AACAAAGTCGCGGTAGCTCA	TCGCGCCCCGACTATTATAA

 

Amplicon 5'

Amplicon 3'

TCCCGCAATGTCCATCTGTCCATATAAACGTCGAGTTCTCGGGAACTATAAAAGCTAGAAGGTTGAAAAGGAAAAGGTGGAAAAGGCTGTTACTGCTGCCTTAGCATAACAAACCAAAGCATACCAGCCCTTGGTAGACAAAGTCGAATTGCTATCACAGCAAATGACAGGAACGCATATCGTACCACCGGTAGTAGAAAGTTTCTCAGATATAGATATAACAAGCGATGCTGGATGTAACAAGCCATTAGATGCAGTTAAGAGCTTACCCGAATTCACAGCGGTACAAGAAAATGGCTTACCGCGTCTTTAGACCACACTACGGCAATGCGCGCCATTACCAGGAGCTCATAATCATAAGAAACAAAGTCACAGGGACCTCAGATGATGAACTAGCTTCATTCGACTCTGCACTTAACGTCGACGCAATCATAAGTCGTCTCGATTTCGCTTACAGCGATAAGCGGCCGATTCATGTTATCGAACAAAAGTTGGCCAGGCTCAGGCAGAATGGCTCACCTCTGCTGAAATATTATGATGAGGTCGGCAAGAAACTGACTCAGTTGACAAATAAAATCAACATGTCATATGAGCCAGCCTTAGCAAAGGCACTTTGCGCAAAATTTCGCGAAGATGCCCTACGAGTCTTTGTGTCAGGGTTAAAGCGCAGCCTCACGGACGTGCTCTTTGCAGCTTAACCGAGGGATCTGCCGTCGGCTCTGCCACTCGCCCAAGAGGTCGAGTCGAACCACGACAGGTATGCCTTCGCGGCCAGCTTCGCGAAAGTCATGGAAGAGAGAGAGCAGAGGGTCAATGACAATCAGCGAGCTAAACCAAATCAGCACGCCGAAGCGGAATGAAGCGGGTCAAAAAACCCGCACTTTAGCACTCAACACAAACAGCAAGCCCACCCC

AACAAAGTCGCGGTAGCTCACCGCAACCTATGGAAGTCGATCCGTCGCTTTCAAGGTTCAGGCAGCCCGCTCAAGCATCGAACGATGCCAACAAGCGGCCAGCCACGTCCGAGCGCACTAACGCTAGTAGTAAGAACCAAAGGGTATATCATTTCACTCAGGGTGCTGGCAAAGACACCAGAATAACAAGATGCGTAACGCCTACGATTTTTTGGCACACGATTTTTTCGCCGTGGCTCTAGAGGTGGCTCCAGGCTCTCTCGAATTTTTGTTAGAGAGCGAGAGAGCTGAGAGCGCTACAGCGAACAGCTCTTTTCTACACATAAAGTGATAGCAGACAACTGTATGTGTGCACACGTATGCTCATGCATTGTAAATTTGACAAAATATGCCCTTCACCTTCAAAGTTCTTTGACTTTAAATCTATATTATTTTTGATCAATTGGCACCATGCGAAAAATTCTTGTTTTGCTTTGGCTTACCGTTATTATTATTTAAAAATAGAATAGAAATAGCCAAATCTATGTACATATTATCACAAAAATAAATTTCAAACTTTATGTAAGAATATTTGTCATTAGAGTATTCATCTTGAGGCGTGTGAAAAATTAATAAGGCAATGATTGTTGAGGGCTTTTGTCCGCACTTCGTGCCTCAAGATATGAACAAAGCAAAGACACTAGAATAATTCTAGTTATCATATTTTTATAAAATTTATGAAATTACGGTAGTTATAATAATTTCTATTGTACTTTCTTTAATTAATTAGTATATTTATTAAGTCATTTGACTTAAAATGATGTAACATTAACATTAAAAGTGTATCAAAAAAAATATTTCGCTTTTAAAAATTTATCTGACAAATTAGAATTAAACATAACAAATTTTAACAAACAAATTTAAGAAACTTTAAAATTATAATAGTCGGGGCGCGA

 

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