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CRIMIC CR01399

Gene CG30427

CG30427

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:24933845..24933867	60E5

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GTCGAACACCAGATGTGGTAAGAG	TCAATGACGACGGCATTTGTGTGC

Images

Construct Design Sequences

sgRNA sequence	AGAAGCGGAGGCGCATTATTAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTGGTTCATGTGCGGTGTA	GCTTCTGCCGCCTTAAGTCT	AGTTAGAGGGGAACTGGCGA	ATGCTTCTTGCGTCACTCCA

 

Amplicon 5'

Amplicon 3'

TGTGGTTCATGTGCGGTGTACGTATTAAGAAGCGCGGCAGCAGAAGAGTCAAGCGGGGAGCCCGCGCCGGATAGGAGGACATGAGATCAACCAGTGGCGATGTCATAAAACACACGGAAAGCGTAAACACTTGACGAATTAGCGGTAGGTGGCAAGAAGCGGCTTAAGACTAGCACCAAAGCAACCCCATACCCAACAAGTACATTCATTATCATCTGCTAATGTCTCTGAAGATCTGCTAAAATCGGCGAACTCTGCCGAACTTTGCTCGCTAGTTAGCAAACGAGGACGTAACCCGTCCATATTGGGTGTATCCATGGGCTATTTGCCCAGCTAAGCAGGTCACCCTTGCCTTGAACCCTCCCGGTTACAAATGAAATATTCATCCACTCCTATCGATTTCAGCATTCAGCTAGCCTATCATTAAATAAGTTTGAGGGGAGCTGCCATCGGAAATACCTATTCACAAACATTTTCCTAACCTTATCTGGGCAGTATAAAAATAAAAGATTCGATTAAAGTATACTTTCATATCAAATTACCACTCGGCTTAGTGTTTGATTTTGGAGATTTGCATCAATATGGTCGGACTGGTTTCTGCCCAATAGTTTCTCCACCTCCCATGAATAACGCCCTCTCAGGTTGGCCAAAAACTGCTAATAGAAAACTTTAATTGACTGCCAGCGCAGACAGATCGTACATTCCAGGCAGAGACGTCCAACAAAGACGCCAGGTGGCCGAAAAAGATATAAATAATAAGCAAGCCAGGGCAACATAGACACAGACACAGACATAGACATAGACTTAAGGCGGCAGAAGC

AGTTAGAGGGGAACTGGCGACTGGCAATTGCAGACTCAATTTCGCTTCCAGCGCAGCTGGCTACCAAGCAAGCAAGCAATCCCGTCCAGAGACATAGAGAATTAACGACTCTGATAAATCGATTCGCCGCAGGCCAAAGCGACGCGGATAGGCAACAGCGAGGTGGCATCCGTACTTAAAAGGTCACTTACCGATGCTGGGACGTGCGATGATGACGGGCATGGACTCGTAGTGGTCGCGCACCAGAAGCTCCGCCAGACGCTTTGAGTAGGTGTATGTGTTGGGGTGCGGCTTTAGCAAATCCGGCGTAATGGCGTCCAGGGTCTTCACGTCCATCCACTCGGCCAGGCGCATAAGATCCTCCGGCTTGTGTGGAAACTCGTACACCTTCTCATACATCACATCCTGGTCGCAGTTGCAAAAAGCCGTGGACAAGTGGATGAAGGCCTCCAGCTGCTTCATTTCCTTGGCCACGTTGAGCGCTCGCCGGGTGCCTAGCAAGTTCATATCGATGGCGTCCCGAAGGTTGCCCTCCAGCTTAAGGGTGGCCGCCATGTGGAAAACAATGTTAGTGTTCTCGGTCACGTGCTTCAAGCTCTCCCCGCTGAGCCCGAGCTCTACAAATGAAGGGCAGGAAATGCAAATCAATCGAGATTAAAGTGCGGAACTTAAGCGCTACACTTACGGTCGAAGGTGACGTCGCCCTGGTAGATGGTAACCCTCTTGAGCATCTCCGGGCGCTCGTCCTTGATGCGCTGGAAGATGGGCAGCTTGAACATCTCCTCGAGACGCGTCTCGGGTGCCTTGCCGCGCTTCGGGCGGCAGATGATGATCACCTCCTTGAGGGAGTGGCAGGAGTAGAGCAACTTCTCCAGCAGAACCTTGCCCATGAACCCAGACGCTCCAGTGATGAATATCGTCTTATCCTTATAGTATTCCTGCACTGGCGATAGATCCACCGTCATCTTTCTGCAAGAAAGAGGTGTATTAGCACACCTTCTGGGAATGGGAATGGAAATGGAGTGACGCAAGAAGCAT

 

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