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CRIMIC CR01402

Gene nahoda

CG12781

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:22907061..22907081	59B4	FBti0210349	86362

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	ATTGCCTAACCGATGCTCGCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGTGCAGAGTGTACAACCCG	GCCGACATGTGACACAAGAG	TCGGTTAGGCAATACGGCAA	GGCCAACTACAGGAGCAAGT

 

Amplicon 5'

Amplicon 3'

AGTGCAGAGTGTACAACCCGTTGGGCGACATTTTTGTGCGCAGCACTTTTGCCCATATTTTTGTTTTCATTTTCAATTTCGTTTGCAGTTTCATTTCGCTTTGTTTTGCCCGCTTTTTTAATTATATATACGCGCGCACATTGCGTATACGCAACGTGGGCAACGGCGGCACGTTTAACCCATTTTGGGGCATGACAACTTTTGCCTTCGCCTGCGGCTGCTGTTCGCTGATTTCTGTTTGCTGCAGCTTGCCAAACGCGTCTCGAGCTGGCCAAAAAGCAGGTCTTAGCCATGGCAACTGCAACTGCAACTGCGGTTTGGGCCGCTCCAGCTCACTGGCCGCCTTGATGATGATGATGTATGCGCCGACGAGAGGCAAAGTGGAATGCAACATGCTGCAAGTGAAAAACTTTCACTTGTGCATTACGGCACACAGAAGTCGCCGGGGGCAGAGCGTGTTGATTGGCGAGTGGCGACTTATTCCGGCCACCATCCACGATTACGAAATGACGAAAATGTTGACAACTACATCGATGATGGTAAATAGAATAACTTCAATAATGAAGGATCAGCAAACATATTTGCAATTTAACTTACTGTAACAGTAAATACCTAAAGCTGGATATATCCAAAATACAACTGCTTAAAACCTAGTCTATCGAGTTCCACGATCTATCTGCAGATAGGTCGCTGTATACAGTTGTATCTGCAACCGACAGTGACAACCAAATGAGGCTGAGGTTGTTTTGCTGCTGCTGCCCTTTCTATCACCATCATCATCGGCGACTTGCAACTGGTTCACTGGCCTACAAAAACGCAGGGATTGGAATGGGGACCAACTGACTGACTGCTGACCTCTGCGCATCGTCTTGCATCTTGTTTGCATTGCGCTGGCCCCTCTCGAATCCATTCCAGCCTCTCAAATCTAATCCATCTCAATTTGTTCGTGTTTGACACTCTTTTCCACAGCCTTTGTCTCAGTGTCTGCTGGCACACGATTTGTTTTGATTTGGCTTTGTTTTCATGTTTCTTTTTTCATGGCTTGTAATTGTCTGCTGGCGTGCTGAGTGCTCTGGAATGGAGGTCAAGTTGTGGTCGCCTCTTGTGTCACATGTCGGC

TCGGTTAGGCAATACGGCAATCCAGCCATCCAATGGAGTTGACATCCACATCGGTTCCGATAATTAGAAGAGCAATCAACTCAATGCAAATGTCAATTAGTCAGCCGCAATCGCAGCAACCGCAGCCACCGACTTGAGCAACATTCAATAAGCTACGAAAGAGATTTATCATCGCAGTTGCCGCGACTCGCGACAGAAACCGATGCCCGTTTATCACTGCAAACTGCGGTCGAGCTGATATTTTAGGCTAAATCCTGCAGCGAAATGTCGCATTGTGAACCATTCAGAATATCAATGAGAGCGCCGGTCATAAATGACATTTAAGCCGTCGACACGGACATCGCTACGTATACGTAATATTGCTGGGGAATGCACAAGTAGCCAGCTAGCACACACTGCAATGTATCTGCACCCCAGTGCATGTGGAGCCATAAATACCAACCGGCAAGTGGAGTGGACTGTGGGGCCAAGTGTGGAGCAGTGGATCGGATCGCAATGGATTGGATTGGGCTGGATCGGAGCGGGTCGGTCCGAGGAGGCCTCCTAAGGGGCTGGCCGAGACAACGTCACGAGCAATTAACTAAAGCGAAACTCCTTGACCACAGTAGCAGTAGCTACAACACAGGGAATGCAAATGGTGCACAGAGCAAAATAACGTCTGATTATTATTGGATTTTAGGAATATTTCATTTACTTAAATCGAATCAATCTGTTCCTGATTCAATATTCCGTTAGACTTAGGTTTTGTGCTCCGTGCAACTTCAGCCACTTGGTCAGGTTGGTTTGCGGCAGTGGCGACCACAACGTCGAACGGTGGCAACAAACTGCCTGTGTAGGGTTGCCAAAGCGAAATGAATTTTATGGCACGTTACTTATGCCACTGCGCACGCCCCTCCCCCTTTTTGGCGGCAAGAAATGCTGAAATTTATTGCCCTCCACGGCGCAAGCGCAACGCTTCGTTAGCCGAGGCAAACGCTGTCTGGCAGTGTATTTGTCAACCTTTTGCAGTGCACCCTGTATAGTACAGTACAGTGTCTGCAAGGCGGAAGTCTTTGTTTACGGCTTCGTGAGAGGCGGCGACCATATTTGGCTGTTGATTTCTTCTTTTCTGCTCACTTGCTCCTGTAGTTGGCC

 

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