CR01404

CRIMIC CR01404

Gene CG12920

CG12920

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:10069719..10069735	46E1	FBti0212924	91252

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GACGACAGCGGCCAAACTGAGATATT	ATGGAGCTGAGCTTGCGATTCTTGTG

Images

Construct Design Sequences

sgRNA sequence	CAGACGCACCTCATCCAAACTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGATTGGTGTCCACATCGT	GCGTCTGGCTTGGGATTTGA	GACGATCACGAATTGCTCTCA	GATTTTCCTCGCCGTTGTCG

Amplicon 5'	Amplicon 3'
GGGATTGGTGTCCACATCGTTTGACTTTAAGCTGGACATCCTTTAAGTAAGATGCATTTATTTACAAAACCAATAACGTCAACAAATGTTTACAATATTTAATTAGAGATGGAAGTTCTAGATCGATATATGCTCAACTTGTCCGATAGCCCGCAAGAACTTTTTTAACCGGTTCGATTTGGCGGGGTTTTACATAACTTTTGAAAACGGTTCTGGGAAAATCGCCAATTGTCAATTACCTTACATGTAGGCTCATATTTCGTAATGGATACTAATTGAACCAAAGACAAAAGTGGCTAGAAACGCGGGCACGACTTATGAAAGTGAAATTGCACTAGTTTAAAGAAATTTCAAATGCATGTCTTGGGCATTAAACTCGTCATCGCGGGCAATACGCATGTGTGAAAATCTGCGCGCAAAGCCACAACAACGACGCTTCAATTATCACTCTGAACTTGGAGCGGATCACCGATAGCCGGTGGTATATAAGCCGGCTACCCGGCTTCGAGCTGCATCATTCGGTTCTGCAGTGACCAACCAAAGCCACAGGGATACCAGCTTTTGCTCTTTACACCGCTACAACGCTAAACAATCATGCACAAGAACACCATTTGGTACGGAGCTGTGCTCCTTGTCGTCTACTTCACGCTGGTGTTTTGCGAGGAGACGAAGAACGAGGAGGCTCCGGCGCAATTGGATAACCAAGTGGAGGACACATGGGAAAATCTCGGCCGGGAGCTAATGGAGTTTGAGGGACGTACTCGACGCCATCGTCACCACATGTTCTGGTGTAAGTAATATCGGACATTAATTTAAAGAAAACAACTATAATTTTAGTTAGCTATCTTAAACTCCCAAAATGTGTAAACATCTCACCCTATCTCTGCAATTCTTAGATTAAACAGAAACATCCTATAAAAGTTACCTAACAATAATCACCACGACACTTTGCAGTACATAGATACTAAAAGATGTTATCAAAAAGCCTAATTTATATAACAATTAGCGAGCAAGTATTACAATTATTCTGATCGTTCGGAATCAACATAATCACATCTCTTGGGCGTTAACCATCCCACTTTCTTTCGAAAGTTTCCGCACAGTTCAAATCCCAAGCCAGACGC	GACGATCACGAATTGCTCTCAATTAACACTTTTCACTTTCAATCTTGGGCAAAGATATTGGTTAATAAATTGTCGTGAAACCTTCTTCAAAAGTAGAAGTAGAAGTCGAACAGTGAAAGTTTTCCAAATCTGATGCAATGGAAAATTATTTAATCGTGTCGATATCATTGTTATTATCTTTACAGATCGTCACCTATGGCCCATTGCCATTGCTTACTGGATCAAGGTGAAAGTGGTGATCGTATCCTTCTTTGTGGGCAGTGCCATCTACTTGGGTCTGCGCTATTTCTGGCCCCATGCCAGGTGCAACCAGGAGATCATCCTTGATCATCCGTAAGAAGCTCTCAAAACTGCGTCCCTTACTGGTTTATTAATAATTCCATTTCTTTTTTAGTCCTTCGTCTTTCTCTCATCACGATCACATTCCCTACTCTCTGGACCACGATCACTCGGAGCACTATGATGCTCCTTCATCTTTTGACTCCGGCGCCAGTTTCGAGCCCTATTCCGGATACAGCAGCTATGCTGGATCGGACATTGTCTCTGATGTGCATGGCGACTACCATAGCGAATCTCCCTCTGGACCAAGTGGCCCATCTGGTCCCGTAGACACGCGCCGTCGCGGACGCCGTAGCGCTGCAGATAACGCCCATTTGATCCAAGATGAGCAGGAGGAAGAGCATGTGGAGATTGTGGATGAGGAAATTTCAGAGAGCGTGGCTCGCCAAATGCCCGCCGAAGAGCAGTAAGTACAGAACGTTTCTTATTCACTATATAAGGTAACATTTTCAAAATTCATCTTTAGGATTGCCGATTTTATGTTCGCCTTTTTGGGCCTGGACTCAAAGGCGTGCCGACGCCGATTTGTCTGCGAAATGGAGTTTAGATCCAAGTTGAATCCCTTGACCAGCATGGCTTTCCGCATTGTGGGTCGCGGTTTCTTTGAGAAGTACACCAATGCCAGAAATCCTATGGCCAGGGCCACCAGCTTTGGCGAGTGTGCTGCCGTCAATCCCGAGTGCATTTTCGTGGAGAACGACAACGGCGAGGAAAATC

Amplicon 5'

Amplicon 3'

GGGATTGGTGTCCACATCGTTTGACTTTAAGCTGGACATCCTTTAAGTAAGATGCATTTATTTACAAAACCAATAACGTCAACAAATGTTTACAATATTTAATTAGAGATGGAAGTTCTAGATCGATATATGCTCAACTTGTCCGATAGCCCGCAAGAACTTTTTTAACCGGTTCGATTTGGCGGGGTTTTACATAACTTTTGAAAACGGTTCTGGGAAAATCGCCAATTGTCAATTACCTTACATGTAGGCTCATATTTCGTAATGGATACTAATTGAACCAAAGACAAAAGTGGCTAGAAACGCGGGCACGACTTATGAAAGTGAAATTGCACTAGTTTAAAGAAATTTCAAATGCATGTCTTGGGCATTAAACTCGTCATCGCGGGCAATACGCATGTGTGAAAATCTGCGCGCAAAGCCACAACAACGACGCTTCAATTATCACTCTGAACTTGGAGCGGATCACCGATAGCCGGTGGTATATAAGCCGGCTACCCGGCTTCGAGCTGCATCATTCGGTTCTGCAGTGACCAACCAAAGCCACAGGGATACCAGCTTTTGCTCTTTACACCGCTACAACGCTAAACAATCATGCACAAGAACACCATTTGGTACGGAGCTGTGCTCCTTGTCGTCTACTTCACGCTGGTGTTTTGCGAGGAGACGAAGAACGAGGAGGCTCCGGCGCAATTGGATAACCAAGTGGAGGACACATGGGAAAATCTCGGCCGGGAGCTAATGGAGTTTGAGGGACGTACTCGACGCCATCGTCACCACATGTTCTGGTGTAAGTAATATCGGACATTAATTTAAAGAAAACAACTATAATTTTAGTTAGCTATCTTAAACTCCCAAAATGTGTAAACATCTCACCCTATCTCTGCAATTCTTAGATTAAACAGAAACATCCTATAAAAGTTACCTAACAATAATCACCACGACACTTTGCAGTACATAGATACTAAAAGATGTTATCAAAAAGCCTAATTTATATAACAATTAGCGAGCAAGTATTACAATTATTCTGATCGTTCGGAATCAACATAATCACATCTCTTGGGCGTTAACCATCCCACTTTCTTTCGAAAGTTTCCGCACAGTTCAAATCCCAAGCCAGACGC

GACGATCACGAATTGCTCTCAATTAACACTTTTCACTTTCAATCTTGGGCAAAGATATTGGTTAATAAATTGTCGTGAAACCTTCTTCAAAAGTAGAAGTAGAAGTCGAACAGTGAAAGTTTTCCAAATCTGATGCAATGGAAAATTATTTAATCGTGTCGATATCATTGTTATTATCTTTACAGATCGTCACCTATGGCCCATTGCCATTGCTTACTGGATCAAGGTGAAAGTGGTGATCGTATCCTTCTTTGTGGGCAGTGCCATCTACTTGGGTCTGCGCTATTTCTGGCCCCATGCCAGGTGCAACCAGGAGATCATCCTTGATCATCCGTAAGAAGCTCTCAAAACTGCGTCCCTTACTGGTTTATTAATAATTCCATTTCTTTTTTAGTCCTTCGTCTTTCTCTCATCACGATCACATTCCCTACTCTCTGGACCACGATCACTCGGAGCACTATGATGCTCCTTCATCTTTTGACTCCGGCGCCAGTTTCGAGCCCTATTCCGGATACAGCAGCTATGCTGGATCGGACATTGTCTCTGATGTGCATGGCGACTACCATAGCGAATCTCCCTCTGGACCAAGTGGCCCATCTGGTCCCGTAGACACGCGCCGTCGCGGACGCCGTAGCGCTGCAGATAACGCCCATTTGATCCAAGATGAGCAGGAGGAAGAGCATGTGGAGATTGTGGATGAGGAAATTTCAGAGAGCGTGGCTCGCCAAATGCCCGCCGAAGAGCAGTAAGTACAGAACGTTTCTTATTCACTATATAAGGTAACATTTTCAAAATTCATCTTTAGGATTGCCGATTTTATGTTCGCCTTTTTGGGCCTGGACTCAAAGGCGTGCCGACGCCGATTTGTCTGCGAAATGGAGTTTAGATCCAAGTTGAATCCCTTGACCAGCATGGCTTTCCGCATTGTGGGTCGCGGTTTCTTTGAGAAGTACACCAATGCCAGAAATCCTATGGCCAGGGCCACCAGCTTTGGCGAGTGTGCTGCCGTCAATCCCGAGTGCATTTTCGTGGAGAACGACAACGGCGAGGAAAATC

Search Again