• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR01405

Gene hig

CG2040

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:9245105..9245136	45B7	FBti0210350	86363

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CCTGGGGACCCAGGATTTATGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATGGTGGCCCTACTGAAGC	CCCAGGATGGATGATGATGTT	ATGGGTGAAAGGCAGCTCTG	AGACATGCTTATAAGGTGCAAGG

 

Amplicon 5'

Amplicon 3'

AATGGTGGCCCTACTGAAGCAGTTCCAAATTCTACCAGAAATCGATCCGAAAACTATTGTTTTTCAAACTGGACTAACACTGCGGACCAAGAACCAGATTCACGTGAAGCTCGTGAGAAGAAAATGAGGCAGCTTTAACGGAATGGAATTGTTCACGGAACTCTGTTAGTCATAAAGTGCACAGTGAACAATAGTTTCATCCAAAGACTCCTTTCTTCTTTCTCTTGGTGCAAAAATGTCCTGTGACTAGCTTATGGCCGATAATCGTTGGCATCTCGACAGCTGCCGCCCTATAAATCATTCATGCCCTGGCGGGTAGAAGCACTTCTCTTCTTCTTTTCCAGCAGCTATTACAATTCCAACTCCCATTTCCCACACAGTGGGAGCTCCCTGTCATTTCCTACCCCTCCTATTATTTTTGAGGCAGCCCAGGAAAAGTCAAGTAATCTCTGTCCTTTAGCTCACATATATGGGAATGCAAATGCATAATGCGCTGGCGACTTTTTGTCATTTGGTTTTCCTCCTCCTGCCGAGGCTACATCCCTTTCCCAACGACTCCTTTTTGCTTTAACTGCGATTTTGACAGTTCGTTACTCAGTTATACCATTTGCATGCATAATGCAGACTCAGCTTTGTTCTAAGCACATAAAATGTGGCAAAAAGACATGGGTGACCATGGGCTCGCAAAATGCACTGTGTGTGGTGCACTGAAATAAGTTCCATCTATCTTTGGCTTCCTATTTATTTTATGAAATATTTATATCATGTTTCGAAGGGATATTTTTAGTTAGCTTCTCTTTTATATGTGGCTTACTACTACTCTACTATTGAAAATAATTATCGTAAACGCATATTTTTTAGTGTGCGCCCAACTCGTTCCAACATCATCATCCATCCTGGG

ATGGGTGAAAGGCAGCTCTGGTTTTAGCTGCTACTCGGCTACTGCATATTTCATTAGTGCTCTGTGCGCTAGTGCTTCGTCGCCATAAGTCAACATTAAATGGATTCCATTTGTGAGTCATGTAAATTAAATTTCCCTCGCTTTCACATGTGTCAAGCGAACGTTTGGATCTCAGAAAATCTAAGAATCGGCACCCCTCTCACCTGACTGACTGCCAAACTGCGCACCAAAAGGCGAAATAAGAAGTCGCAATATCCGCCACAAAAGAAAAAAAAAGAAGATAAGTGCGACGCACTTTTGCCTGACTGAAGGACGAAACCCCAAAAGCTCAGCAGCTCAACAGCTGGTCCTGTCGATTTGCCAGGGGAAATTAGAAGGCAACCGACTGGAGGGATGGCGATGGCGATGGCGATGCCGATGGAGGCAGAGTGAATTAATTACTTTATGCGCATCTGCGTGTCGCTGTCGGGCACTTTACACTTCCCCTGTATCCTTTTTCCCACTTCCCTTGTCCCTTGTACCCCACATCCACCCATTCCCCCGTCCAAAGTCTTCCTTCCCTTTCGCAAAACTCACACATGACGCTATTAATTTCTATTACCAGCCTCGGACGGCCTGCCTGCCATACTTCATTACGGACTTTCGTGCAATTGCCGCTTGCCCGAGCTACCTTTCAGCTCCCCTTCCCCTAGTGCACTGAGAGAAATTATTGGTGACTGCCAGCGGAATTAAGTTTCGTTGAGATTTAGGAGCAAAGGTCTAACAATCAAAAGTATGCATTAAGTTTGCGCCTTATTAATGATTTCACATGTTCAAAATGTTCAGTAATCATAATTTATAATGTATAAATATACATATGTAATTGAAAATATATTTGATCAAAAATAACTAAATTTATCATTAAATTCTTACCTAATAAATTATCATAATACGCTCGAAATAAAGGTTAAATAATTATTTTACAAGGTGTGAAATACATAATGATTTTAAATATTTATGTGAATGCTATACGAAAATTTATTTCGATTTGTCATACATGACAATCATGTTTTTCTTTATTCTTACTGAAAAATAATGTTTGTGATTGCGTTTGAAAACTAACTTTAAAGACTTCATGATGTCTTGCTTTGAATGATTCATAATAAAAGCCATTATCCTTGCACCTTATAAGCATGTCT

 

---

Search Again