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CRIMIC CR01406

Gene CG15083

CG15083

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:18835671..18835699	55F6	FBti0210351	86364

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CTTAACGGTACCTGCAACCAACATGG	AGCCCAATAGCCAATAGCACCCTGTA

Images

Construct Design Sequences

sgRNA sequence	CGATGTCGTCATCGATGGTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGAATCACGCCCAGCCATC	GGTTGTGAATTGGAACGCG	TGACGACATCGCCGACAAAT	GCTGGCTTCGCTGTTTTTCA

 

Amplicon 5'

Amplicon 3'

TAGAATCACGCCCAGCCATCCAATGACCACACCATAGGTGTTCAGGCGCATACAGAAGACCCTGGACAGCATTCTGGATTCACTTGATTTCGTGCTAATGCGAAGATAAAGACAATTTTACTTCAGTTCGCCGGCGCAGCAAAATTGCAATTGCTTGATAAGTCGCAATTGGCTGACAATTAAAAGCTTTTGGGTACAGCGGCGTTGCCAGACTGGTTCAAGCAGTTGGTGTCGAAACTGTGACTTGTTCACCAGCTTTTAATGTGACTTTTTAACGCTAATTGTTTAAAATTCTTTTAATAACATTTTTATATTTTAAATATTTTTATGCATTTATTATTTGGCATGATAGTTAAAAAATATTTATTTATGTATTCATTAACACTGCACAGCAGCGGCTTACTTTCATAATATTTTTCAAGGTGCTAATAGTAGCTACATTACATATATAATACAGCATTAAATCTCTCTATTATTTTAAAGACAATTCCCCATTTTATTCATTTCACTCTTAAATGACGAAACATATGCAAACAAATTCATATAGTAAATGAGTGCACATATATTTTTTAAGAACATATCAGACGTAGTTTTTCCACGCTCGAAAAAAAAGTATTTTCGTGTTGCATAGTTTACGCAACCGTTTCTTCTGAGCGGCATCGGCATTTGCGCCACACTTATTATTTGATTCTTGCCCAGTGCGGTTGTGTTTCCGTGCGCTAGGTGTTCCCAAACCGGTTTAAGTGCCATACTAGCCATATTTGTATTTGGAAAATTCGCTGTAAACCACAGAGTTTCAAAATGAAAATCGATCTGACGGTGGAAAATGGCCCGTGCAATACCGATTTGCGGAGCTGGTGCCTGGGAATCGCGATCTATTGGCTATTGAGCATGACTTTCAACGTTATATTCGCTCCAAACGGTAGGAGAAATGCGAGAGGCGTGGCATTTGTTTATTTTCAGAAGCTGATTGCCCAGACTGACGCTATTTCCATAGAGAGACAATACATTCATGATGTTCACATGCGAAATAAACGAATGAGCTAATCCATTCGACACAAACGCGTTCCAATTCACAACC

TGACGACATCGCCGACAAATTCGAGCTGACAATTCATTTTAGGTGGCCCGAAATGCCATTTTCTCACAGGCTTGAATTTTCCAGCACAAAAAAGAAAATTAAATAATTTCCTTGGGCTGTGCATGGCGCAGAATTAAACTTAAAAGATTATTGGGTTTTAACAGTTGAACGCCATATGTCATAAAAACACTTAAAGCCCTGCATTTGTCCATGTCTCAAAGAAGTTCTATAAACCTTATTCTAAGCTGTTTTAAAAAGTTTGTCATATTTCATGTATGAAATGTGTTTAAAATATAAAAGAAGAATTTATGTTTTAGTTCTTTATGTATATGTACACATGTACATGAAGTGCATCAGTGCATCAATATGTACTCATTATATACGAAGATACATTTAGCAGTCGTGTTCTCAAATTTCAGACGGGAAGAAAGATAAGAGCACAGCAACGTTTTGCTTGGAATACATTGAGTCACTTATAAGCCCAAGTTTATGTTTACTTTCCACTAATTGTAACGGCTTTTTTTATGAATGAAATATGTACGTTCAATAATTTCTATTTAAATTCACTTGAATCCTTGCAGTTCTTACCTTTATTGGATTTGCCATCGTAGTAGTCGCCAATATTTGCTTATTGATAGGAACCCTGAAGGTAAATAAAATACAACCCATTCCGTTTTATTGAGTCAACGAAACCCAATCTACCTTTCCGAATTGCAGGAAAAGCCAATTCTGGTGTTCGTCTGGCTGATCTTCGCTGCCATTGAAGCCATCTCCTTTCCGTTTGCCGTCTTCGGGGTTATCTTCCACTACGGAGGATATCGCGAGGCTACCGGAATGAACAACGTTTTCGGTGCCCTGCTCGTTTACATCATCACATTTCGTAAGTATCGGCTATGAACATCAAATTGAGTTAACAAAAATGTACAGATCATACATATTCTCCGCTACACTTTCAGTATTGATCCTCTTCAGCGCACGAATTGTGTACTCCTTCTACCTCCAGCTGAAAAACAGCGAAGCCAGC

 

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