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CRIMIC CR01407

Gene Ack-like

CG43741

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:13152115..13152135	49F4	FBti0210352	86365

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACTCGTGCCGATGCAGCCAAGATAAA	ACTGATCTGAACTCACCCGTTCGTTG

Images

Construct Design Sequences

sgRNA sequence	CTGCACCCGCATTTGTCATCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCAATTCGTTTCTGCGGGGA	GGGTGCAGCGACATCAAGAA	AAGGGGCCATTGTTTGTCCT	TGGCTGAGATCAACGAGCTG

 

Amplicon 5'

Amplicon 3'

TCAATTCGTTTCTGCGGGGAGTAAAAAGTATAATGAGTTAGTTCGGTTGAAGACAGGGGGGAAGTGCTTCCGCTTTTTGGGTCACTCACTTGACGGCATTGTAGTACTGCTGCAGTTCGGACTCAGTGAGGAATTCGTATAAATCAATTTGTGGATACTCTGGAACGGGGGAATGTACAAAAATAAACACATTAAGATCGTGGGTATATATTATACAGATCTATATAAGCGACCGCACAAGACCAAATCTCGGGGGACCTTATCTTTGAAGTGAAGAGCGAGTAGAGGGAATACCCTATGCAATAAATAACTTCTTTAACGATCGATCCGCCAACGCAGATGGTTTTTTGAATATGATAAAATAAAACACACACATCTCCTGAATACCCAAAAGTTCTGCCACCGTCCCCGCACCCTCCCCACCATCATCTCACACAGGAGTACGTGAGCACATTGACACAGAATTCCGATTGCCTTATTTTATACCCTACACTCTCTCATAAAATGTATACAACCCTCATCCAATTCCTCCTCTTGAAGGTGAGGTTTTCGGAAGTTTAATGATTAAAGAAAACAACTTTCGTGCTATTAGGTCTATCTAATTAGACGCGCCACTTCAATTGAATCCTAACCAGAAATTAATGGGTGGTTAAAAAAACTTTCATAGGTTATACTGATTTATAAGTCACAATACGTTGAATTAGTCTGATTGATCTAATTATAAATCGCTTTAATGACACAAAAATGATAATGAACTTCATAGGCAAACGAAGAAAGTAAGAGTATCTGATAGTCGCGTCACCAAATTCGAAGGTTCTTTCTTGATGTCGCTGCACCC

AAGGGGCCATTGTTTGTCCTCTCCGGAGTTGACACAGTGGGTGGGCAAGGGGTAGGTTGGAGTGGGAGTGAAAGCGGGACTGGGATGCGGGGTGGAACTTAAGCAACAAATGCATGTACACAGATTGTAAGCTGATATCGTTCCGGGGATTTCATGGGCGATTACTGAGTCATTCGGCTTGGGAACGGGAGCTCGCTCTAGTTTCCGGCGCCGCCCCGATTCCCGTTTCCTCTAATCCCGGCCGCACTTTCCCGCAGGACTTCGCTGTCCTTGAATATGCAAAATAAAATCGATCGGCCAATACATACACGCACACACACACTTGCACACGCAGGCGGAAGCCTCATCGCAACTACGTAGCAGGAAACTGCAGTTGGAAGTCGTTATTCTTGCACTTTTCACATGTCACCCACGAGTTCATATTATTAGCCGCACAATAAGACAATTTTCCAGTTTTAAACAGCTCTTTTTCTGTCAAACTTACCCATTTTACACTCTCGTGTGCGTCTGCATTGTTTTTTGTTTTTTTGCTGCAATAAGTATGACCGTCACGCGGAGTCGGCTCAATTACCAGGCACCAGTGCTGGAAAATGCTATGTTCAACATCGTAACGGTTTGGCGGGATTTTAAAATTCACTCCTTAATAAAATTGTTCAATTCTCATTTCCTTTTTTCTTACCGGAAAATCAATCGATCATTAATCTTAGTTAAATTTAATAATTTAGTTTTAATGAAATGACTAACAATATTGAATTTCAGTTAATAGCATTTATTCATATACATATGGATTACATTAGTTATACAAAACAATGATCGCTTCGAAGCGTGAATATTTGTTGCGTATTCTGTCGGAAAGTGCTCAGAACAAACGGAAGCTGCTGGGAAAGGCAGTGATCATCATACGGTTGATGACAGTACGGATGTGCTCGTGACCCAGGGCGCCGTTGATGGCGGCCAGCTCCACGAAGTGATCGCGGGTTCGCTTCTCCTCGATTAGCTTAACCACATACTTGTACATGCGACCGTAGTGGCCATGGGCATAGGCGTGCCACAGAGCGAACTGGATGGCCTTGGAGTCGTTGGCGTCCACGAACTTGATAATCTCGGTATACAGCTCGTTGATCTCAGCCA

 

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