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CRIMIC CR01409

Gene CG13321

CG13321

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12953770..12953773	49E4	FBti0210353	86366

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GATTTCGGCTGTCAGGTGCGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAAGCGCCCATGCTCAAAAA	CGCGGAGTATCGATGACACT	CCTGACAGCCGAAATCCCTT	CACCCATCCGTACCCGTATC

 

Amplicon 5'

Amplicon 3'

GAAGCGCCCATGCTCAAAAAATCCCCCCCGTTGAAGGGGCGATAAGCATGTCGAAAATTTGTACTTTTAATAGAAAAAGGATCATTCATTTTGAATTATTTTTTACCTACTGAAATATTAAAAATAATCTTTAAACCTTTAGTGTAATGTATGTGCTTATTTTATCTCTTTTAATTAATAATTTCTTTGTAATAACAAACAAACTGATTTCAAAGGACATATGTATGTATGCATTTATGTAAGGCATAAAAGGCATTAAAAAGTATAAATATACATATGTGACTCAGCGGTAACGATATCAATAACTTTATATCGCAATTGACTCATCTTTAGACCAAACAAGTGTCTGTAACTTTACAACAGTTACAGTTAGTTTTCCAAAGGTTTCTCAATTATTAGGTTTATGATTTGAATAATCCACAAGAAGGTTAGGTAACTGTTTACATGTTGGTTCTTATTTTTGTTCTAGAAAAGCATTCCTGAACTTATAGTTGATCCATGGACAGCCACCCTCATACATATAATCTTTTACGAACTTATTGATGAAGTTTATTTTTGACTACCGGCGATAAGATTCGTAGCCAACTGCTTATAGATAAAACCCGCAAAAACAAAAACAAATATTCATGCACAAGATCGATTGAGTAATTTTGGTTGCGCTTCCTGTTTAACCCAGAATTTAAATTGTTTTTACCAAACAAAATCATCCAATATACATTCTGACACACCTGTCAGTTGGTCGCAATGACGAACACCCTCTACGACATGAGGATACCTTTGTGGAACACACACAATCGAACGGTTCAGAGCAGTGTGACTAAGATATCTTATCACTGCCGGCTAACTTGCCGAACTGATAAGCGGAAACAACAGCTTTTAAGGCGCAACCGATGTGCGGGTAGCGACCAGTCGTTGAAGTCAGCCCAAACATAGCGGAGCCAGCGAGTATATAACATCACGTTCCAGACCTAAATCAACATACAAAAAAAGCACACAAATACAACATGGGAGGTGAGAACTGAAGTGCAAAGTTAGTGACTAAGATAATTCTGGCGAAAACGAAAGCATAGTGCCGCAATTAGTCGTGACAAGTGGGCAAGTGTCATCGATACTCCGCG

CCTGACAGCCGAAATCCCTTGGAGCTTTGGTAGCTTTCCATAGATCCAGCGATTCTACACCCTGACAAATAAAAGACAAAAAACCTCTACTGTGGCTGATTAATGGTGTACAGTTGGCCCTCGTTGGCCCAAGTTAACTTATCGCCTCTGACCTTGCTCTATATATATATTTTATTCGCCCTCAGACTACACCTGGATTAGCACCAACGTTTACGGATCCCTGCCCCCAGGAGCCATCCTAGCTGGCCACGACTCCGACCAGGATCCCATCTTTGTGGGCCGTGCCTACCACAATGGAGAGATGCTGCCAGCGAAGGTGGTGCCCGGCAAGCAGCAGGCCTATGTGCCATGGGGCGGTCAGGAGATCAGCAAGCATGACTTCGAGGTCCTTGTCGGTGATCACTTCTCCTGGATCCCGTCCAGTGGTGGATCTGTCCCGCCCCATGCCATCCAAGTGGGCCAGACTGGCGAGGGTGAGCCCCTGTACGTGGGCCGTGGTTACTTCCAGGGCAGCCTCACCCCCGGCAAGGTCCATCCCTCGCACCAGTGTTTGTACATCCCCTACGGCGGACAGGAGGTAGGTCTAACCCTCACTTTGATGTTATGTTGATGGATTCCCTTCATCCCCTCAACAATAACGTTCGAAGACCACGTAGTGACGAATCGCACAAGCAGTGTTTGCAATGTGATAAATCTTAGTAAATCACTTTTATCTTATATCATTTCAGCACCGTCTGGAAGCCTATGAAGTGCTGGTCCAGCCCGAGACGTGGATAGCCTCTAGCGGACGCGGCATTGTTCCCGGAACAGTGGTTGGCGGACACGACGCCGATGGCGATCAGATCTATGTGGGTCGCGCCTACCACGAGGGCGATTTGCTGCCAGCCAAGGTGAGAACCTTAAACTATATACAGCTCGTGTTCCGAAAATGTACACTTGGAGCATTCCATCTTTAAATCTGTTCTGTTGCATCCCACTTTATCTATCCACTTATCCACCACAGGTGATCCCCAACAAGGGCTGTGCCTATGTGCCCTACGGAGGCGGTGAGGTTGTGAAGCACGACTACGAGCTGCTGGCCGGATACGGGTACGGATGGGTG

 

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