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CRIMIC CR01410

Gene qvr

CG33472

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:11447421..11447451	47F13	FBti0210354	86367

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
long band	OK

Images

Construct Design Sequences

sgRNA sequence	GTACCTAAATGTCTGTCCATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCACCAAAGCTTTCGACCTT	GGTACAGTCACGTCCCAAGT	CCTTTGACTGATTGATCGACACC	TTTTGGGGTCCAAGCGAGAA

 

Amplicon 5'

Amplicon 3'

GCACCAAAGCTTTCGACCTTCTTTGTTTAGTTTTATTTTCGTACTACCCTCTAAAAATTCATTGAATGAGGTTCAAATCTGTGGTAAAGAGCGTTTCGGAACAAAATAGGTCCAATTTAAAACCTAATTAAAAATCTTCTATATGATCAAAGATCTGAAACAAACTATACCCGGAATATTAAGATTTAAATCATATTTACCTCTGTACGCCTGTATGTGTATGCCTGTATACTTCATCTGTGAGAGTGCAAGCATGAATAAAATTACACTTGCCATATAATACATCTCCAGTAAATCCAAAGACATAAGTGTGTATTTTGTTTTGTAAATTTTTCTTTGCAATGTATTTCAGTGAAAAAAGAAATGTTGTTCAACACTTAACCAATTCAAAAAATTCACAAGAAACCAACTGCTAATTTCCAAAATTGTATCAAGAAATGTTTTCTGCCTGCTGCTTGTTTGCTCGTTGGCAACTCTTGGCATGCTTTTGTTTGTTGCACGTTTTGGAATTCCTGTACCCAAACTAACCCAACCTCAAATTAGATCGAAGAATAGCAGAGGAGCGTACTATAACCAGAAGTTGACAATAACGATATATTTTTTTATATAGCGCGATCGATTTACTGCTATGAGTGCGACTCCTGGACTGATGCCCGCTGCAAGGTAGTTAATAAAAATGAATGTGGGTTATATGTGAATTACTCTAATTGATCTATTTATGCAGGATCCTTTCAACTATACAGCCCTGCCAAGGGACCAACCTCCCTTGATGACCTGCAACGGTTGCTGCGTGAAAATGGTGCGGCATCAGAGATCACGTGAGTACTTAGCAGCCCCGACACAACTGTACCACCGGACTTGGGACGTGACTGTACC

CCTTTGACTGATTGATCGACACCCTTGTGTGTGCGTTTCGCCGTCTTTTTCTCTCTTCTCTCGCTCTCTCTTTCAGCGTACGAGGTGGTGCGACGCATGTGTACGTCACAGTTACAGATCAATTTATTCATGGTCGATCACGTGTGCATGATGGAAAGTTCAGGCAATGGACATATGTGCTTTTGTGAGGAAGATATGTGCAATTCTAGTAAAAATTTACACACTAATGGTTGCCAGCTGCATCTCATACCCATCGCAGTGGCAGTATCTTGGCTAATGGGTCAATTGCTCAGCAGATAGAAAGTGTTAGGCTCACCCACAGAAATCCACATACTCAGAACATACAGAACATACAACACAGCCAGAGAATACCCTACACACTCACTATCTCTCTTCCTCTCTATCTAAGCATATGATTTGTAGATTGATTTGATTTCATTACGATAACAGTTACGATTGATTTGTTTTGGAGCACAGTCTGCAAAGGTCGGACTTCGAGTAGCTTCGATCAGCACTAAGCGAAGACTCGAAAACCCAATCTACGGAATGTGAATGCATACCAAAGCTTCCAAAGACAGAAGGCTTTCTCTATTTCTCTATCACACACAGTAAACACACCCTAAGCGAGCTTGGGATGCACAGAGTTCTTGAGATGCGAGTTTAATCGAATCGCATTGAAGTCGAATCCGAATCGAATCACAGTACTCGTATTCGGTATTTGAATTGCATGTTGTTGATGCTAAAGCTGAAGCATGTAGCGCTTAAGTAGGAATCGTAGTGGTTATTACATTTTGATTTCGTTGTTACTTACATTTGTAAATGCCCCGACCAGAGGGATAAACCAAAAACCAACGTCAGTAATCGATTCAAACTTTAATTCGTTTATAGACTTACTTTGAACATTTGTCTAAATCATATGTAAACTTGGAATAACTCGACCCCAGACACATTGTGTATATAGCCAGACCCAAAAACATCAAAGATCATCCCAAAAGTATTGCAGTTACCAAGTGGAACATTTGCTCATCTTGTTTATTCCAAAGTCGGAGGAAAACTCCATAATGCATAAACGTTACTTTGTTGTTAATACTCTGTATCCTAGATCTTAGCCATTAAGCTGTACTCTCGGTTCTCGCTTGGACCCCAAAA

 

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