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CRIMIC CR01411

Gene CG13148

CG13148

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:12687166..12687184	49B12	FBti0210355	86368

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGAAGGGCAGCAGCTTTTCCTGCTTT	CTGCTATCGTTTGTACGTTCAGCCTC

Images

Construct Design Sequences

sgRNA sequence	GTTGGTCTGCACAGTCCTAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGGTGCTACATGGCAAATCG	GGACTGTGCAGACCAACACT	TGCATACCTACGCACATAGTGT	TCGAAGAACTCCCAGTGCAC

 

Amplicon 5'

Amplicon 3'

CGGTGCTACATGGCAAATCGAAGGATTTTATTTAGTCATTCAATATTGTCGCACTGCCGATACTCACATCACGCTCTTTTCCACCGCAGGCATTGTCTAGGCTCTGTCTCTTAACAACTACAATAAAAAATTATTTAATAACAAATGTATTCTGTTCCACGCGCAAATTCGCGGCGTTGGAGTTGCCACCTAGCACAATAAAATGCCGGGACAATCAGCTGGTAACACCGAAGGTGTATCGATAAAACGATAGTTCTTGTTTTAAAAAGCCAGTTAGAAAATTGTGATACAGTACTTTATACATTTTCTTTAGCTTACTAAATCTTACTTAAATCAAGGTATGACATCTTAGATCTGATGATCGCTTATAGATTTATACAAATAAAATATACATGGAAAGCACGTGTGCGCCCCATTATTTATCTATTATTGTAGCATTACAGTTAAATTGGTATATATTTCACGCGAATAGGTTTAGAAACCTATTTTATAGGTTTCAAATATAGACGTTCGCATGTGGCCCTTCAATCATAACTTGACACATGCATTTAGCATTCAGATCTTGATATTTCCCAAATATTTCATAAATAGCCTTCCAATCCAGCCGGACTAGAACTATTTAGGTTAGACTTTCCAATCACATAAAGCCATTCCAGTGAGGCTGTGGCTGCGGCTAACAGACCTGCGAAGTCAGAGTGCAAAGTGCGCAGATGCTGCACTCCGGCGCCCACTGCACCGCCCAGCCACCCAACCACGCCATTCTAGAGGCCCATTTCTTGGAAACTAATACCACTACTCCTCGGCTAGCCGCAGCCTCCCATCTTTTCCAGATCTAAGAGCGAGCGAGAGAGGAGCGGAGAAGGCGCGAGAGAGTAGGCGCACTCGAAGTTCACGTTCACGGCGCCTGGAAGCTGCAGCCCGAAAATTGTCCTGTTAGTACGAGAAAACTATTGACAGGCGAAAGGTCTAAAATTGCTCATATTCTGGCAGAAAAGGCAGATTGGACTTCAAAAGTCTGATAGCCGCAATGGCTCAAGTTCAGCTCAACGCAGGAAATGGGCAAGCCCCTAGCAACGGAAATAATGTCGAGGATAATGAACGTAAGTCTGCCGATTGGCAATTGTGAAATCATAATAGAAAATTGAGTGATTGCCCGGAAAGTGCACAAGTGTTGGTCTGCACAGTCC

TGCATACCTACGCACATAGTGTGTGTCATGTATCAGGATATATGTAGGTATATATGTATGTATCATAAAGGTGTATGTCAGCAGCAGCTAGCATCTTTGACATAGCTAGGATGCTTTTTGGCCTGGCGTTGGGTGATAACTTAAACGGGAGAGCGAGCATGAGAGCAGGATAACAAAGAGAGAGAAATGTCTGAAAGGATGCTGCGAGAGCGCCAGAGATGCCACTGCTGCCCAAAAGCAACAAGTTTTTGAACTTAAGCTAAATCAGATAAGGCTAGAACTACCTCGATTTCGTTAGTTAATTTCGTAGTGTATAAAGGGGTATCAAATATAATTATTATAATCTGTAAGCTTTTATATTATTCAAAACAAGGACAATAATTAAAGGTAACTTATTTCTTTTATTTCCATAGCCTATCAAATAAGTGACGATGATTTGGATGACCTGGATGATGATTGCTTGCTCGAGGAGGCAGATACCTCAGGGGCAGGCAATAATGTGGGACGTGGTCCTTACGAGCGTGCTTGGACCACAGAGGCCACCAGGGCATTGATTCACATACGCGGTCCCATGGAGGGTAGCTTTACAGAGGGCAGGTGAGCAAGGTTTTTATTCCACCGTAAAGACATCTTTGTAAAAGTAACTCCCTTACAGACAAAAACGGACTGCTCTTTGGCTGCACTGCACCCGGCAACTACAGCGACTGGGATTTCGCTACTCCGCCGCAAAGGTGCAGAAGAAGTGGCATAACATCCTCATCACCTACAACAAGAATCTGAACAAAAAATACGTATCCGGCTATGTGCACTGGGAGTTCTTCGA

 

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