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CRIMIC CR01412

Gene CG2269

CG2269

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:10090873..10090875	46E4	FBti0210356	86369

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TCGGCGTAACGTGTGAGCAAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CACGCTGGATATTTGCAGGC	AAAGGTGAAGGTCTGCGCTT	CTCACACGTTACGCCGATGT	GCGCTGTGCTTTCGACTTAC

 

Amplicon 5'

Amplicon 3'

CACGCTGGATATTTGCAGGCACATTGGAATTTTATTGATTTATATTCCTTGGTCCTTTCTATATGCATGAGGCTGTGGGATTCGCTTCATTAATCAGTCAGGATGAAATGGGCAAATTATTCAAATACTTCGTGCGGCGTAAGTCCGGAATTTTCGGGTACCTGCTGCTCGTTTGCATAATTAAGCGAGGAAATATGGAATTTCACACGCCATCAAGTGGCCGCGAAAAGATGACACCTGATACACACTCACGAACACACACACACAAACACAACGACAAAAACCCACCCACACTGCGACACATACATTCATGCACGGAAAAGGACAACGCTTTAAAGTGCAAATGTAATTAAAAGTTTTTTTACGCACCTGTCGCAAACGTGTCGGGCATTAAAAACCATTTTAATCCCGTTCGTTGAACATTTAACTGGTTTTCCTAAGCCACGTTTTCCGATCGAAATTTTTAAACAATTCAATGCGGTTTTAAGTGGTTTTAGTCTTTAAATATCGCAGTTTTTAACATAAGCTTCGTATATATTTACAGATAGATACAAAGTTTAAGAGAAAGAAATAATTTAAAAAGCTATTATTTTTTTCATTTGCTGTTTGCTGCTATGATATAAATTTCCTAGACTAGACCAAGGACCTTTACGAAAGTGCTTTCCTGTGCATCTTCGAAAATAACTTATGAATTTAATATTTTTTGCACTATTTTTTTTCCAGATTTAGTAGTTCAATTTGGTGTCAGAGTGAAAGAAGGAAAGCAGCCAGCGAGGTAATGGCAGCAATGGCCAACCGATTTGTAGGCCCCAGTGGTGCCATGTTGGTGCTGCTGGCGGTGGTGCAGTGCATCCTAGCGACACCACAGCGCACCGCCGCCTTTTCGCTGCAGGCGGCCGTGGATGAACTGCATCGTCGGGAGGCGGCCAAGTATCCACTGAACGCCCCTCCGCAGCCAGCTCTCGACGACTGGGATGAAGGTGAGTTAGGGGGGCTGGGCGAATCTGGCCGGGAAATTGGTTTTCCTCGGGGAAAAGCGCAGACCTTCACCTTT

CTCACACGTTACGCCGATGTAAGTTAATCAAGCAATTCCCATGTAATTTGAACTTTGATTAACGTTAAGGTGAAGGTTGAACTAGAATGAACGCCAGACAGGACCTTTAAGATGCTCCAATCTACCTTGTAGTTCATCAATTTTTAATGCATACCCTGTACTTGTCGATTATAGTTATCTGTGTGCTTTTTATTATTATGGAAAATTATGGAAAACTTTGTTTGAAAATTGCTCGTATATCATTTTCAAGAGTGGAAGTAGTTTGGAGTAGATGTCTTAAAACTTATGCGGAAAATAATTACTATTAACTTAGATATATAGAAAAGTAAATAGCAATAAAGTAGTGTATTTGAAGTGCTTAAAAGTTTATAAATTAAAATTTGAATTTAAAAATATTTAACAAATGGCTCAAAATCGGAATGAAATAGTATTTGTATGTGCATAAAATCCTAATAATTATATTTTAAATTCATTTGGGAGTGAATTATGCCATTTACTCTAGAAATGTCTGTTCAGTGAAGCATATTTCGTCATATGCCAATTCATATATGTACATATATGCCGAAATATTACTATTCGATTTGTGTGGCTGGTTCTGAAGATGACGGCCGTCAACTGCATGTGTAGATTATGTAACCGCAAGTGGTTGCTGAAGTCTGAAAGTGATATCCATCACAGGCAAACGTAGATGTCGGGAAGCCGCATGCGGAGTGCACGTGCCCCGGATAGCCGATCCCCAAGCACAGATTTACCGGATTTTCCCCTCACAGTCACATGTATCGCTGTTACTTTTCCTCGTTTCCATTTTTCCTTTTCTTTTCTCTTTTGGCCACTCCACCTCTTCACCAAAACCCTCTTCACCAACCCGAAACGCTCGCACAGATGGCGATTTATTTGGCAAGAATAGGAATCGGAATCGCCATCGAGTTAATAAGGCACTTGGCAAGTACTTAGAACGCGACGAGGACAACTACGGTCCGGATCACAACTTGGGCTTGGGCTTGGATCTGGGCATGGGCATGGGTCTGGGACTCGATGACGATGGCGACTTGGTCCCCAGTAATCTGGTCGATGAGAAGAGAAAGCGTTTCTCCTCTTTTCGCGAACGTGACGAGCAGTTCGGTAAGTCGAAAGCACAGCGC

 

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