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CRIMIC CR01416

Gene CG32260

CG32260

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:4143904..4143923	64A8	FBti0210358	86371

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK

Images

Construct Design Sequences

sgRNA sequence	ACCGAATTGGCAAACTAAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TACCCTCAATCCGCTCCTGA	TGCCAATTCGGTTTGCTTTG	AAAAGTCCTGAACCACGCCC	GAGGTGATTACGTAGCGCGA

 

Amplicon 5'

Amplicon 3'

TACCCTCAATCCGCTCCTGAGCTTGGTCATTTCAGTCGACTCTTTGGATAATTGTGCCCCACTAAAATACAATTGAATTATAATCATTTTGGGCCGCCATGCTCCACTTGGCCAAGCTGCAATTGGCCGAAAATTGAGAGTTCTGTGGACAGTTGCCACCTGGTGGCTAAGCAGAAAGCACGGATAAACGGATACGGCTACGGATTGGAAATGGTACTGCTGGACGAGTTCTGTGTGAAGGTGTACTTCTGGCCATTCCTTTCGTATCTGGCCAGTATCAAGGGCTGGTGCCCGCTGTACCCACCAGGTTACTATCCCATCTACGCCCTGCCGCCGCCGCAGGGACCTCCATACCCTGCGCCGCCCCCGCCGCCGCCGCCGTTTCAGCCGATTGGTCCACTAATACCGCCCCAACCCGCTCTTCCGTCCACACCTGCAGTGATCCCGACGTTCCAGCCAACGCCTCCGAATCCCCAGACGCCGGTGACGCCGCCCCTGATCACGACCAGCAGCAGTACCACTAGTAGCACCACATCGACGACGGGACTGACCACCACCACGACCGCCGGCACCACTCTGCCGCTTCCGATGCCAACGTGTCCATCGCAGCCGCTGGTTTGCCTGACCGGCGGAGCACGTCCACTGCCCAACTGCCCCTGCATCGATCCTCGCCAGCAGCAGAACTCCCCGTTGCTCTCGTCCACGGGACCACAAATCGGTTCGGATATGATGGTCTTCATGCCGCTTAATTCCGGACGTAGGCGACGTCGTCGCCGGAGACGTCGGATACTCTACTAAGATCACCTTCTAGCCACCAATTCAAAAAAAAATCACTTACCCCTACACTGAAAAAAAACGCACTTGCATATATACAGCAAAATTAGTCAAAATAAAAACGATCACAAAAACCAAAGCAAACCGAATTGGCA

AAAAGTCCTGAACCACGCCCCACCCCAATCACCTCCTTCTTTTGCAGCTCAATCCTGTCAAGATGCCCGATCCCGGCCTGGAAGCTGCCTGCCCCTGACCTCCTGTCCCCAGCTCATGCAGGAGTACCAGGGCCAGGCCAATGAGTTCCACACCTTTCTGGGCCAGTCCATATGTGGCTTTGATGGCTCCACATTCATGGTAATTTCTCAGTTTTTTTTTCGGAATGTTATGGCTATTAAATTTTATTGCTAATTGGAAAATTGTTTAAAACGATTATCTGCAACTACGCATTAAATTGCATTTATTTTTAGACGATTGCTTATTTGATAAACTTTTTGCCTAGAGAAAAGTTGATACTATATACATCACAAATTGGCAGAATTAATTTTAAACGTTCTTGTTGTATTAATAATAATAATTTATAATTAAGTAATAATTTGTTATACTTATAAGTAAGTAAAAAGTTAGGACTTCTTATTCGATTAGGTCTGCTGTGCAACGGATCGCAGTGGAAATGCCAGGAGCAGGAAGGATGTATTTGTGACCACCGCAGCTCCTTTTGGATTCTTTCATTTCTCGCCCTTGAGTGGCGGTTCTACGGCCACCCCGATGGTTTTCCAGCCCACGCCGCCCCTCAGCCAGGTGGTGAGTCCCAGCTTCTACCCACCACCACCTCCGCCTCCACCAAATAACGCTCCACGTGAGTCCGCGACCTGCGGCATAAGTGGCGCCACTTCCAATCGAGTTGTGGGTGGCATGGAAGCACGGAAAGGTAATGAAGCTAAATAGAGTTGTTCATGTATTAAATGAACTTCCAACTACCAGGAGCATATCCCTGGATAGCTGCCCTTGGCTACTTCGAGGAAAACAATCGGAATGCATTGAAGTTCCTCTGCGGTGGCAGTTTGATTCATTCGCGCTACGTAATCACCTC

 

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