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CRIMIC CR01417

Gene Pi3K68D

CG11621

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3L:11786699..11786704	68D6	FBti0210359	86372

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	AGGCGAGCGACAACTTGCTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCGGGAACTTTGAAAGCCAG	AGTTGTCGCTCGCCTTTAGT	ACGGGCAATTCTAAAAAGGATCT	TTGCAGGTGGAGTTGTTGGT

 

Amplicon 5'

Amplicon 3'

GCGGGAACTTTGAAAGCCAGCAAAACAAAAAGGGTAAGCGTACAAGTAATTTGACAAAAGTGCAACGACTTGAATGCAAATCATAGTAAAGAGTTAGTTGATTTTCCAATCTAGCCAGAATGAAATAAATAAATACATACATATGCTCGAAAAGCTTCAAATCTATTGTCAATTATTAGTTAGAGAAGCGTGAATGTGCGTTATTTAATCTTTTAATGGGAAATGGAAGCAAGCGGCTAAACGAATAATAAAACAACAACAGACTGCAATTTGTTTAAGAAATTATTTATTTGTTCTCTCACAATGGTACACGATAGAATTAAACATATGTATGTATTTATTTTTGTATATAAGTGTATTAATGGATATATGTAGCAGCAAATCACAATTTGTTCATTTCGTAATACATGGTTATTAAAAAGAAGTGACTAATTCTTCTCATTTCATCACTTTTCAATTGACAGTCGTTAAATTCTTTTAAATTTTGAAGTTTTCAGTCGCGGGAATATACATAGAAACCAATTCCAAACATTGCGAACTAGTTGAATACGACATTTTGGACATCGATATATAAGCTGACTTCAAAATTATTCCGGCAATAGCGGAAGGCACGAATTTCGATATGAGGGCACATACATATATGTATATATATTACTTTAGTATTTTTGTGTAATAAGGTGGTCTAAGTAAGTGCTGTTATAGACACTAATCTGTCATTGCCTCCGAAACGAACCAATCTGAACCGAAACCCTTTAGATGGTGTAGTTGTTGTGGGTGTAAAGTAGTTGCTTTTTTGCATTTTTGGTAGTAGTAGTACTGATAGTAGTAGAGGACAATTAAAATGGATTACTTTTTCGACACTGCAATATAGAGAAACGGAAACATTCAGTTTAATTCTTAACTAAAGGCGAGCGACAACT

ACGGGCAATTCTAAAAAGGATCTCGTATATCTAGCCAAACTAATTGTTGCTAAACAAATAATGGGATAAATATAAAATTAATAAAACCTATTAGTAAATACCATACAAATACAAAATCGTGTGCTTGTTCCTATTCAATTTAATATTCCTCTCTCCTAGAGATTGAGTTGAAAAATCCACTAATGTTATTATGCCAATAATTTTTATTTGCAGCTCAAATGAATGCTCTGGCCAAAAATCGCAACACACGAGTTTTCGACTGTATTTCAAGTTTAGTTTACGGTGCGAAAAATGTGGGGGGATTTCAACGAGGACCGGGAGCTACACGTTAATTACAACATTGAGGCTGCTTACCAGTATTGTCGTTTAGCAGGGGATCGAATGCCTCCAGCACACTCACACGGGAGCTGCAAAGCAGGATGTTTGAATGTTAAAAACACGAACTAATCATTTCTGAACACTAGTGGATACATACTAGTCCTCGTGGTTGAGGTCGATCAAATCGTTGCCCCTGCGTGGGACCGAGGAATGACCATTGTTTCCAGTGTGGCTACTAGAGCTAACCCCGACAGTCGAGGGCACTTGGCGACGCGCTGGGAAGGCTACTCCATTGGCCTCTGCGGGGGAGGAAGTGGAATGGTTGGACGGTTGGCTGTGATGATGACTTGTTGTCGGTGCTGTTTGTTGGGGAGCTGGTTGCGATTGGGGTCGACGCAAATCCATGTGCGTGTAAGCACCTCCAAAGCCATATCCCTGTGCGGGATACTGCGGCGGCATAAAAGCAGCCGATCCGGGAACAATGGGAGCCGTGTATGGAGTAACCACAGCTCCGGGATAGTACATATAGGCGTTTGGCTGCGGCACTGGAACCACGGCCATTTGAGCGGGCATGCTGTACAGCCGCTGCAGCTCCTCGCTGTTTAGTGGCCTCTGTTGCTGAGCAGCTGGTTGGTAAGGCACCAACTGCAGGTCACCGTAAGCCGGTCCACCAACAACTCCACCTGCAA

 

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