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CRIMIC CR01419

Gene CG1113

CG1113

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:5253748..5253785	82F7	FBti0210360	86373

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	CTAATGAAGGACAAAACTCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGATCACGGCCAGATCGAC	ACGGAAGAGCAAGAAATCC	AGTCTTTGAATGAATGTGCAA	TTGCGGACGAGGATCATGAT

 

Amplicon 5'

Amplicon 3'

ATGATCACGGCCAGATCGACTAGGCTTTTGACCCTGATCAAGAATTTATAGGATATGAAGCGTATCTATCTACCCTTAACACCCTTTTCAACGATTAACGTATAATTTATTAAAGAATTTGTATTCATTGAACCATAAATTTTAAGACCACTTAGTACCATCCTACTACATATATACAAATAAACGATAATGAAAGTCTGCATAGTTGCTGTCCGATCATAGCAAGTAATTCATCAATTAAAGCGTAGCCCAAGGAGCACGCGAAATCTAAAAAAAGGCCAAAAAACATAATTTCTTTAATTGGTGCTGGTAAGTGGCAAAAATTATACAAAATTCTTGCTTAAAATCGCATTGATTGATTCCCCATTTAAGCACTCAGTACGATTTGATTTAATTCCCTAACTTGTAATGAATTTGAATAAATAAAAAACTCCTTACTTACTTATTTATTTTAAAACCATATCATATTAAAACTTGGTTAACTACCGCTTGATTGGTGTATGACTTGTTAGGACCACAAAATATTTTTTTGATTTTCTTTACAACTTGGTCTGCGGAACTTTCACACAAACATCAGTATATCAATCAATTAATACATTCTGGGGAAGGGTATCTAGCTTACTGGGACCTGAACTCAGATTTTCCCAGCCCTTGCGGTTGCGCAGACGAAGTGCATCCGAAACCAGGACAGCAATGCGGTGCGTGACTTGGCGAATGGTGTGCCCAAAACGGAAGTGCACAAAAACAAACAGTGGGAGGTTACCGCTGGCAACAACAGTATGTTGTGCTTGGTATTGATAAAGCGCGTTTGCAACAATTAAAGATTTTCCAATAGGAACACTTCAGCTTCCCGCAGCATGAGCTCGCACTATCTGAATTCCTGTGTGAAAGTGCCCATCGATCCGTGCAATAACTGTCCGGCTCCGGTATACAATCGGCCCAAGCTCAGCTCCCTTGGTGGAAAGAAGCCGACCGACCAGGCTCACATTTCGCAGCCAGAGGCAGAGACACCTGCGTGGAGCAGCCAGATGGGGGTCAGGTCCCTAGCTAAATTCTACGACAGTATACCTGGTGAGTTCAGCTTGGAAATGTCTGCACCTTGAAATGTAATCTTCGGGGTTAGTATTTAAAGGATTTCTTGCTCTTCCGT

AGTCTTTGAATGAATGTGCAATGATTTAAAATAAGAAAGATGGATAATTAGAAAGACACATACTCACCACAATTATTTTGCTTTCCCTAGACTATCGTGATGTGAAGCACCTGCACCGAGTTGAGTTCTACTCAACTCTGGAGAGCCTGCGAAGCACCAGCCGTGAACTGCGATCTCAGGCAGCAACTGCTCCAACTGTGGAGCGAAGTGCCAGTGTCAGCAGCTGTTCTGTGGGTCATGGAAAGTCTAAAAAGCGGCCTTCCAAGGGGAAAAAGATAAAGAAACGTCCTTTGGTGCTGGATCCTGTTCCTCCGCCAGCAATCTTAGTGATCCCCGGCCAACAGGATAGATGCGCTACCCGCCCAGTTGTCCATACTCCCAATTCCAGTTGTCTAAGCGACGAGTACGACCAGTGCCTCAGGGACTTGAGTCGTCTGAAAAGTTTTAAGGAGGACTTTGCAGTGGAGGTTGCGGATTTCAAGAGGTCCCTTAAGAGCTTCGAAGCGGAGTTCCGCAGGCCAAAGTCCACGAGCAGCAACGGAACTCAAACACAAACCAACAAGCAAAGTCAGACGCAGAACCGCTGCCAGAAGACTGCCAATCCTGGCCAGCACACGGCAGAACGGGCCAAGTGCCGAAGGGAAATGCTGCGCAGGTGCTTTAGTGTCAATGATCTTCAAGGGATTGCCTTAGAGGCTCATTCGAAGCCTTCCAAACTAGCTCAGCCTGCGCGAAGCAACTATTTCCCACTCAGCAAGCAGGAGGAAATTCCCAAGATACAGATTCAATCACCCACCACCTCGTCGAAGCGAAGTAGTAGTGCTTCCAGCGCAGCAAGAGGTCGAGGAAGACGCGGAAGAGTAAGGAAACCAAAGCCAGGGTCTAGCGTAAAGGATGAGAAGCCACTACCACCGCTGAGCATCTATGAGTTGCCGGAGGAACCGGTCCAGAGGAGAACGCCCAGTGTGTCCCCGTTGCATCCGGTATCTCGTCCTAATCTAGCGGCAACTTTGAGGGCTGAAGTATCTAGGAAGAAGGTTAAGGAGCTGAAGAACAACTGCACTTATCATGATCCTCGTCCGCAA

 

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