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CRIMIC CR01422

Gene Pdfr

CG13758

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:2573753..2573781	3A6	FBti0210362	86375

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GAGTCAAAAGACATGTCCTACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTTCTGGAGGTCGTGTTCG	TGACTCCTAATAAGCCGGCAG	ATCCACACGGCATATCTGGC	GAAGTCCTTGCTGCCCATCT

 

Amplicon 5'

Amplicon 3'

TGTTCTGGAGGTCGTGTTCGCCATGCAGACTCATTGGTTGGTTGGGTTTAACTCGTACAAAGTTTGATGGGTTTGGGATTGTTTTTTTTTTTTTTTTAGAACGATATAACTCGATATAATGCTGGTGCGTAAATAACAACGATGTTCATTGTTCGCTTTGCAGCACATTTACTATGCGGTCGATGGCAAAATGACCCTCCTGTCGAACATTCTCGACTGCGGAGGCTGTATTTCCGCCCAGCGCTTCACCCGCCTGCTGCGCCAGTCCGGCTCATCAGGACCATCCCCATCTGCACCGACGGCCGGAACATTTGAATCAAAATCCATGCTGGAGCCAACATCCTCGCACAGCCTGGCGACCGGACGCGTGCCACTACTGCACGATTTCGATGCCTCGACAACGGAATCGCCGGGAACGTATGTCCTCGACGGTGTCGCCAGGGTGGCCCAATTGGCCCTGGAGCCCACCGTCATGGACGCACTGCCCGATTCGGACACGGAACAGGTTCTCGGTAATCTCAACAGCAGCGCGCCCTGGAATCTAACGCTTGCGTCGGCAGCGGCCACCAATTTCGAGAACTGCTCGGCGCTTTTTGTCAATTACACCCTACCACAAACAGGTACCTAAAGCCTAATAACTTTTAAATCATATTGCAGTATTTTTTTTTTTTTTTTTGTTGCCGGGATAGGACTCTACTGCAATTGGACCTGGGACACATTGCTCTGCTGGCCACCCACTCCGGCTGGAGTCCTTGCACGGATGAATTGTCCTGGCGGCTTTCATGGCGTAGATACGCGCAGTAAGTAACTGAAATACACTCTAAAAAAAAAGCAGCCGAAAAAGAACATGCTGAGTACAATTAGCATAATTAATCGTTGAAGCTGTTTAGCTGTTTAGCAGCCACAAGAAGCTTTATGACTTCTGCCGGCTTATTAGGAGTCA

ATCCACACGGCATATCTGGCGGTCACAATCATACGGATCATTAGCTGGGAAACGGTTCAAGTGGTGCACGGCAAACAATTACTTGATTCCGTATTCATCGAAAGGATGTCAATATTTACATGCATGACTGGGGGCGTCCAATCAACCGCACTCCCAGTCGGGGCCGCCCCTCCTTGGCTTCCATCGCCGGGGGCTGTCCAGGTGTTTCATGTGCACCCTCGAGCTCCGCCAGTTGAACAGGGTTTAAAACACCAGCCAATCGATTGCTTAATTACGATCCTAAATGCTTGCTCTACCCCTCCGATAAATTAAACGTTCTGCAATGGGGAATTGCTATTTAATAACCATGGGCGATATACACTGAGCGAAAAGATTCTAAGTAGCTGACTTACCACCCAATATTTTACAAACTTAAAATGCTCTTCCATTATCACTAGTGAATGTATTATCTGCGCTTGTGGTGCAAAATCTAGGCATATCGAACTATATCTGTTAAGAAATAATTATTCGAGTGCATTGTTGAGCTACGGCCTCATTTCTCCGGGCCGTGTCTAGATTTTTAGCCCCAATATTTTCAACTGGACGAGGTCGGTCGCCCATTCGATTGGCATAATTGCACTTGTCAGTCAGTGTGTCAAAGCTCGATTACTGTCATTAATCCAACCCAACCTCCCTCTTTTTCGGTTCGCAGAATTCGCCATCCGAAAGTGTGAGCTGGATGGTCGATGGGGCAGCAGGCCAAATGCCACGGAGGTGAATCCGCCGGGATGGACGGACTACGGGCCGTGTTACAAGCCGGAGATTATCCGTCTCATGCAGCAGATGGGCAGCAAGGACTTC

 

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