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CRIMIC CR01424

Gene CG13982

CG13982

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:6412928..6412944	26D1	FBti0210363	86376

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGCAGGGCGTATCACTTGTGTAAG	AAGACTCAGATGGCCAGTATTCCTGG

Images

Construct Design Sequences

sgRNA sequence	ATCCGCTGACCAATCAATAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGTCGGTCCTGCAATACCC	GATTGGTCAGCGGATTTGCG	CGGGGTGGAATTGGCTAGTT	CTGAGTCCATCGCCAGTCTC

 

Amplicon 5'

Amplicon 3'

ATGTCGGTCCTGCAATACCCTAACATTTCCCGTTATTACCCCTGATAAAGCTACTGCTAGTCGAAAGCGCTCCGTGCCGTGAATTTATTAACTTTGAGCGTCCGTCTAAGCTTTAAGTAGTGACGAAAGCTCCTGTCACAAGACGCTTTGCTCTCCTGTTGACTTTAGCCACTTGACAGCGCAAAACGCGAAAGCCACAACACTCAGTCGAAACAGCTGACTCTCAAAAGGTGGCCACCACCCACCACCCAACTGCTGCAGGGCCAAACAGAAACTCCGTCACTAGGCTTACGGAGCATTTAGCCAAGAAAGCGTGGGGGCGTCACCGTCACTCACTGACGTCGACGCACTCGGCGCATGAAAAACAGTTACGCTCTGTGCCGCGGAGTGTGTGGAGTTGCGTTGAAAGGGGCTCCGGAGTCCAAAAGGGTAATCGGAAAGCCCACGGGAAGGGGCCATCACCGCGGAGCCTTGACCCGAGCGTAATGAAATGTGGCGCCTGGCTGGCGAAAAAGTGACGTGAAATGCCAAGTGCAGCCGCATCGAAGCCAGCAAGGATCTTGTAGCAGCGACAGGAGCAGCCCCTTCTCCATGGGTTCATGCCTTGGCAGCTGTGCCACAGCTGCCGCCGCACCAGCCCACTCCGCCTGCACCTCAACGTCCGACTCCACCACCCACTCGACTTCCTCGACGTCGTCTTCACTGACAACTCCCCCCACGTCCTGGGTGGCCAACAGCTGGCTCTGGTGAGTGGGGCCAATTAGTTGAAGCTCAACTCCGCTGACTTTCGTTCGCGCGCAAATCCGCTGACCAATC

CGGGGTGGAATTGGCTAGTTGGGGGTCAACAGCCTAAAACTATGCAACGGCCGCTGGTTGCCACAAAACAATGTGCCACAGGCGGGCTTCCCGCCCAAGTGGGTGCTTACTGCAACCAAATACGTAAACAGTAAATACTAGTTATATGTGATATTCTTTAAGAGTTAACAAAAGCTTTTACTTTATAATAATTATTAAAAACATCTTTAATAAAAAGTGGTATCTTTCATCTTCTAGGCGAAAGAACAGATCTGCAGGAAGACAAGATCCAGAGGCAGATCTCTTATCGAGGACCACCAGGCAGCGCTGCCAACGGAACGGATTCATCTACCGCATCCTGCGGCTCAAGAAAAGGCAGCAGTGTCCCAGCTTCCTGGACAAGTACTGGGAGCAGCAGCCCAGCTACGCCAAGCTGCCGACCACGTATGTTAAGTCTCCTAAGGTGCCTCCTAGAAATAAAACCAACTTAATCCTTGCAGCGCCATGGCGGACATTCAAATGCAGAACCTGGACGCCGGCAAGTTGCTCAACGCCCAGACCACGCCCAGTCGGGAGACGGAGATGGGACTTGGAGCCTACCAGCGCATGGCCAACTCGCGGGCCAGCTCCTCCTTGGATCTCGAGTGGGAACACGAGTACTCACAGCTGCGGCAGTATCAGTACCAGTGCCAGCAGGTGCCCAAGGAGCTGCCACCAACGCCACCAAAGCCGCGCTACGCATCCTTAGACCAGCTGGCGGCAGCCAATGCGATGGCCACCAGCCAAGGACGACACATGGCGCGCCAAGCCAGACTGAGTTGCCAGCGCCACGGAGGTTCGCTCACGCGGAACTCGTGCTGTTCGTCTACGCAAAACTCCTGGTCGCACATATCCACGCCAGAGTCGCTGGAATGGGACGTGGACGAGGAGCGAGAGCAGCAGCGTCAGCTTCGTCTGGAGGATGACAATCTGGATGATAGAACGCTCAAGTTGCTGCATCAGATCGAGCAATTGAAGCACCATGTACTGCAGGAGACTGGCGATGGACTCAG

 

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