CR01424

CRIMIC CR01424

Gene CG13982

CG13982

Information

Donor Location Cytology FBti Stock number
pM37-p22L:6412928..641294426D1FBti021036386376

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK

Images

Construct Design Sequences

sgRNA sequence ATCCGCTGACCAATCAATAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ATGTCGGTCCTGCAATACCC GATTGGTCAGCGGATTTGCG CGGGGTGGAATTGGCTAGTT CTGAGTCCATCGCCAGTCTC
 
Amplicon 5' Amplicon 3'
ATGTCGGTCCTGCAATACCCTAACATTTCCCGTTATTACCCCTGATAAAGCTACTGCTAGTCGAAAGCGCTCCGTGCCGTGAATTTATTAACTTTGAGCGTCCGTCTAAGCTTTAAGTAGTGACGAAAGCTCCTGTCACAAGACGCTTTGCTCTCCTGTTGACTTTAGCCACTTGACAGCGCAAAACGCGAAAGCCACAACACTCAGTCGAAACAGCTGACTCTCAAAAGGTGGCCACCACCCACCACCCAACTGCTGCAGGGCCAAACAGAAACTCCGTCACTAGGCTTACGGAGCATTTAGCCAAGAAAGCGTGGGGGCGTCACCGTCACTCACTGACGTCGACGCACTCGGCGCATGAAAAACAGTTACGCTCTGTGCCGCGGAGTGTGTGGAGTTGCGTTGAAAGGGGCTCCGGAGTCCAAAAGGGTAATCGGAAAGCCCACGGGAAGGGGCCATCACCGCGGAGCCTTGACCCGAGCGTAATGAAATGTGGCGCCTGGCTGGCGAAAAAGTGACGTGAAATGCCAAGTGCAGCCGCATCGAAGCCAGCAAGGATCTTGTAGCAGCGACAGGAGCAGCCCCTTCTCCATGGGTTCATGCCTTGGCAGCTGTGCCACAGCTGCCGCCGCACCAGCCCACTCCGCCTGCACCTCAACGTCCGACTCCACCACCCACTCGACTTCCTCGACGTCGTCTTCACTGACAACTCCCCCCACGTCCTGGGTGGCCAACAGCTGGCTCTGGTGAGTGGGGCCAATTAGTTGAAGCTCAACTCCGCTGACTTTCGTTCGCGCGCAAATCCGCTGACCAATC CGGGGTGGAATTGGCTAGTTGGGGGTCAACAGCCTAAAACTATGCAACGGCCGCTGGTTGCCACAAAACAATGTGCCACAGGCGGGCTTCCCGCCCAAGTGGGTGCTTACTGCAACCAAATACGTAAACAGTAAATACTAGTTATATGTGATATTCTTTAAGAGTTAACAAAAGCTTTTACTTTATAATAATTATTAAAAACATCTTTAATAAAAAGTGGTATCTTTCATCTTCTAGGCGAAAGAACAGATCTGCAGGAAGACAAGATCCAGAGGCAGATCTCTTATCGAGGACCACCAGGCAGCGCTGCCAACGGAACGGATTCATCTACCGCATCCTGCGGCTCAAGAAAAGGCAGCAGTGTCCCAGCTTCCTGGACAAGTACTGGGAGCAGCAGCCCAGCTACGCCAAGCTGCCGACCACGTATGTTAAGTCTCCTAAGGTGCCTCCTAGAAATAAAACCAACTTAATCCTTGCAGCGCCATGGCGGACATTCAAATGCAGAACCTGGACGCCGGCAAGTTGCTCAACGCCCAGACCACGCCCAGTCGGGAGACGGAGATGGGACTTGGAGCCTACCAGCGCATGGCCAACTCGCGGGCCAGCTCCTCCTTGGATCTCGAGTGGGAACACGAGTACTCACAGCTGCGGCAGTATCAGTACCAGTGCCAGCAGGTGCCCAAGGAGCTGCCACCAACGCCACCAAAGCCGCGCTACGCATCCTTAGACCAGCTGGCGGCAGCCAATGCGATGGCCACCAGCCAAGGACGACACATGGCGCGCCAAGCCAGACTGAGTTGCCAGCGCCACGGAGGTTCGCTCACGCGGAACTCGTGCTGTTCGTCTACGCAAAACTCCTGGTCGCACATATCCACGCCAGAGTCGCTGGAATGGGACGTGGACGAGGAGCGAGAGCAGCAGCGTCAGCTTCGTCTGGAGGATGACAATCTGGATGATAGAACGCTCAAGTTGCTGCATCAGATCGAGCAATTGAAGCACCATGTACTGCAGGAGACTGGCGATGGACTCAG
 

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