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CRIMIC CR01425

Gene CG17834

CG17834

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:8536133..8536154	29D7	FBti0210364	86377

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCGTTAAGCTCCTTGAATGTCTGCGA	GCTGATGGTCCTGGAAGATTTGCGAA

Images

Construct Design Sequences

sgRNA sequence	ATTCGAGCCAACAATTCACCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCACAAGAGAGCGCGTAAAG	GGAGCAAGTTGTCAAGCGTG	CCTGGCTAACACACATTCCG	TGTCGGTTGTATGCGTCCAA

 

Amplicon 5'

Amplicon 3'

GCACAAGAGAGCGCGTAAAGAGAGAGTTGGCGCTGTTTAATGGGGAGAGCAAAAGAGCGCCTAAAATAGTTAAAAATTGTCGCAAAATCATCACTCTCTGGCAGTAATCTATCATGCGCGATCGATCAATGGCTCTCTAAAAATGTCTCTGCCCCAGCCCACCACCCCCTGCCATAGCTCCACTTCCAGAGAGATGAGTTCATAAAGTGGGTGAAAGAGAGCGTTCTCCAGTAGCTCCTCTCAAACGTACTCACTTTTATCACACACACGTACACTCAAAAAATGTCCATGGAAAAATTATTCTCAGTAAAAAATTATAAATTAAATATTTCTTTACATGATATTATTTAACAAATATTTAAACGAATCGAGACATATTAAAAAAATAGCCGGGAATTGGTTATGGTTTTACAAGGTAAACGTGTCGTATGAGCAATATTTTGGTGCAACGACGCTATTGAACTACGCTTCAATTCAAATAAATAAATTCGTTTTTCTAAAAACATAAAACAAAATATTTGAGTGTTTTGTTAAAAAATATTTTAATATATTCATAAGAACATATTGTTATTTTAAAATACGGTTTCGAAAATCAAAAACCAATTTCTTGAAATTCAGTAGCGTGTTTCCCTCAGTGTAGAGTGGAGAGCGGGTGGAGAGCGCCATCAGAGCGCGATTTGGGGCCACTTCATGTGCCGATAAAAGCTGTCTTCACACGCTCACCGCTCGCCACTGAAAAAAAATTTCTGGAACGAGTCACACGCACCGCACGTTCCTCCGCAGAAAGGCAATAAAAACAATAACAACATAACTCGTCCTCGTCGAACAAATCCGAATTTAATTTATTATTCCCAAACAGAAATCATAAATTAATCAAAGCATTGTTACAACACAACAACGATTTTCGGACTCGGATTCTCTTCATTTTTTATTCGAGCGGAGAGTAAATAAAGTCAAGAAAACAAGAGGAAGAGGAAGAGCGGTAGGGAGCCAACAACAAAATGTGCCGCTGCTGTCAAAAGTGAGTAAACGCTACGAATTTCCCCCTACCCCACCACGCTTGACAACTTGCTCC

CCTGGCTAACACACATTCCGAATTGCCTTTTCTTTATCCATTTCCATTTGTAGCTGCTGGGAAGATTTCTACTGGAATTGCTTCGAGGAGCGTTTCTGCTACGACGAATCCATCGGTAAGTTTTTGAGCTTTTTTGATTTGGGCAGTTGACGGATACGGATACTCTGGTTACGGTTACCTGAAAATGAGTACGAGTACGAGTACTTCGGCTACTGCCACTCAAATACTCACTCTCTTCGCACGCCTCACACACACACACATACAAGAAACTAATAAGATCTTGAAACGCACAATGCTACGGCGATTTTTGCGCGATTTAACGTGTTTGGCATTTGAAATCGAGTACGAGTCGTAAAAGGAAAAAATAAAACACAAGCTGATAAATCAGTTTTATGGCACTTTTGCCAAACTTGCATATAGAGTATCTTAAAAAGTTGCGCTTAAGTAAGCTGCGACAAGTTTTGTTTACATAAATGCTTGCATGTATAGAAAGGCAAAATAGTTTTATTATTTTATATAAAAAAATGAATAATAATAAGTACTTAAAAGAATTTAACGATAGAATATTCCAGAGGTAGAGTTGGGCTTATAGCTTAGATCAAATTCGTTTCTGTAGAAGACTCACATTTTGTTTGAAAATGAAATTCAAGGAGAGAAGACTCGGAAGTTTCTGGAATAAATTTGTTTATAAGATTACGGTTTTTTCATTTACTAATTTCTGCTCTTTCTTTGCCTCTCAGCCAACTACAATCCCGAGGAGGACGAGGACTACTTGGCGTCCAGGAAAAATGGCCAGATCATTGGACGCATACAACCGACA

 

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