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CRIMIC CR01426

Gene NimB5

CG16873

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:13971078..13971082	34E5	FBti0210365	86378

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GGCACAGAGATCGCAGTATTGTTGAC	GCAATGCAAGCCCATTGAAAGTACCG

Images

Construct Design Sequences

sgRNA sequence	CATAAGCATAGACTGAGAATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCTTAATTCGGATCTGGAGG	CTCAGTCTATGCTTATGAATT	TGGAAGCAATGTGGTGAACT	CCGATCAAGCCCAAGGGATT

 

Amplicon 5'

Amplicon 3'

GCTTAATTCGGATCTGGAGGGGGAGATCCAGGGCTCGCAATGCCAATGTATGTCAAGTAAGAACGAGCTAAAGATCGTCTTAAGTTTTATGTTCTTTCATTACGAATTGTAATTTCCCGGATAAATAGTGATGGGATGCAAATAAAATATTTCAGTAACCAAAAGTATCTTTTGGGTATGTAAATTAGCATATTCAAATATATTTTATTTTATTTTATTTATATTTAAATATGCTTTGGGCTCGTTCAAAATAAAATTAGAAAAAATATTTTAGAAAATTATCCAACTGAGGTATAAACAAGTCGCAACACTTAAAAATTTCGACTTACATGTATTCATAATAATAAACTCTCATAAACAAACTGAACCAAATTCCTCTCATCTTAGCCTATGCATCGAATATAGCTGCTTTTCTGATAAGACAAAAAAAATTAATTCTGTTTCAGTGGCGAAAATTAGTACAATTCTAGCTGGCAAATTGTGAACACCTAGAAACTTGAAATAAGGAGTGCTCAAGATGTGGTTGCGAGACTTGCGCATTCGTCTAAGTTTTTTCCACCTGATGGTGTGTCTGCCCAGTATTTACACAAATGTGCCAAGCTATTCGGATCGGTACAACAGAAGGCCTGTGTCCCAGGATCCAGGAATGGGAACCTATGGCCGCGGGTACATGGAAAACCGGCAGTTGTCCTATAATCGACCTGGACCAGCCAACTTCCAAGATCCCAGAAATGTGGAGTTGCAGCCGAAACGACGAGAGTATCTCATTCGATCACATGAAACGCAATCCGATAGGGGGCAACACAAATGCCGCATTTGGGTGCCGTAAGTATGTCAGATACACAGAAAACAACGTTGAAGTATATATACATTAACAAAATTTTTTTATTTTAACAATGTATATATATATATGTATGTTTTGATAATCAGAAATATATATATAAACTTTCTACTGTGAACATAATATCGCAAGCCTTATCTGTACTCCCAGAATTTGTAAAACTTTTAGTTGCCAAAATTCATAAGCATAGACTGAG

TGGAAGCAATGTGGTGAACTTAGTTATAATATTTTTTGTTTTCCAACATTCGAGCAGCCCCGATACGGTGGAAAAGTACTCGTACCCCAGTGTCATCCAGACGGACCAGGCCAACCGGCTGTCCCTGATCGAGGTCTGCTGCACCGGATACTCGGCCAGTCGTCTGATGGGCGTAACCGTGTGCCGGGCGCAATGTGGCTGCCAGAACGGAAGCTGTAAGATTCCTGGTGAGTGCGAGTGCTATGATGGCTTTGTGCGTAACGACAACGGTGACTGTGTGTTTGCCTGCCCGCTGGGCTGCCAAAATGGTCAGTGCTATCTGGACGGCAGCTGTCAGTGTGATCCGGGCTACAAGCTGGACGAGACGCGACGCTTCTGCCGCCCGATCTGCAGCAGTGGCTGTGGCAGCAGTCCGCGGCACAACTGCACCGAACCGGAGATCTGTGGCTGCTCCAAGGGCTATCAGCTGACCGACGACGGATGCCAGCCCGTCTGCGAACCGGACTGTGGAATCGGCGGACTCTGCAAGGACAACAACCAGTGCGACTGCGCTCCTGGTTACAATCTGAGGGATGGTGTCTGCCAGGCCGATTGCTATCAGTAAGCTGAAAACTTCATGCTGGGAATACCAGACTAATGGCAATCTTTATTCGGTGCAGGAAGTGCAACAATGGCGTGTGTGTCAGCCGAAACCGATGTCTATGCGATCCGGGATATACCTACCATGAGCAATCAACCATGTGCGTTCCAGTATAGACATTATTACTAAAGAATTGATTTTTATTTCGAAATCCCTTGGGCTTGATCGG

 

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