CR01426

CRIMIC CR01426

Gene NimB5

CG16873

Information

Donor Location Cytology FBti Stock number
pM37-p22L:13971078..1397108234E5FBti021036586378

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK no band

Images

Construct Design Sequences

sgRNA sequence CATAAGCATAGACTGAGAATTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GCTTAATTCGGATCTGGAGG CTCAGTCTATGCTTATGAATT TGGAAGCAATGTGGTGAACT CCGATCAAGCCCAAGGGATT
 
Amplicon 5' Amplicon 3'
GCTTAATTCGGATCTGGAGGGGGAGATCCAGGGCTCGCAATGCCAATGTATGTCAAGTAAGAACGAGCTAAAGATCGTCTTAAGTTTTATGTTCTTTCATTACGAATTGTAATTTCCCGGATAAATAGTGATGGGATGCAAATAAAATATTTCAGTAACCAAAAGTATCTTTTGGGTATGTAAATTAGCATATTCAAATATATTTTATTTTATTTTATTTATATTTAAATATGCTTTGGGCTCGTTCAAAATAAAATTAGAAAAAATATTTTAGAAAATTATCCAACTGAGGTATAAACAAGTCGCAACACTTAAAAATTTCGACTTACATGTATTCATAATAATAAACTCTCATAAACAAACTGAACCAAATTCCTCTCATCTTAGCCTATGCATCGAATATAGCTGCTTTTCTGATAAGACAAAAAAAATTAATTCTGTTTCAGTGGCGAAAATTAGTACAATTCTAGCTGGCAAATTGTGAACACCTAGAAACTTGAAATAAGGAGTGCTCAAGATGTGGTTGCGAGACTTGCGCATTCGTCTAAGTTTTTTCCACCTGATGGTGTGTCTGCCCAGTATTTACACAAATGTGCCAAGCTATTCGGATCGGTACAACAGAAGGCCTGTGTCCCAGGATCCAGGAATGGGAACCTATGGCCGCGGGTACATGGAAAACCGGCAGTTGTCCTATAATCGACCTGGACCAGCCAACTTCCAAGATCCCAGAAATGTGGAGTTGCAGCCGAAACGACGAGAGTATCTCATTCGATCACATGAAACGCAATCCGATAGGGGGCAACACAAATGCCGCATTTGGGTGCCGTAAGTATGTCAGATACACAGAAAACAACGTTGAAGTATATATACATTAACAAAATTTTTTTATTTTAACAATGTATATATATATATGTATGTTTTGATAATCAGAAATATATATATAAACTTTCTACTGTGAACATAATATCGCAAGCCTTATCTGTACTCCCAGAATTTGTAAAACTTTTAGTTGCCAAAATTCATAAGCATAGACTGAG TGGAAGCAATGTGGTGAACTTAGTTATAATATTTTTTGTTTTCCAACATTCGAGCAGCCCCGATACGGTGGAAAAGTACTCGTACCCCAGTGTCATCCAGACGGACCAGGCCAACCGGCTGTCCCTGATCGAGGTCTGCTGCACCGGATACTCGGCCAGTCGTCTGATGGGCGTAACCGTGTGCCGGGCGCAATGTGGCTGCCAGAACGGAAGCTGTAAGATTCCTGGTGAGTGCGAGTGCTATGATGGCTTTGTGCGTAACGACAACGGTGACTGTGTGTTTGCCTGCCCGCTGGGCTGCCAAAATGGTCAGTGCTATCTGGACGGCAGCTGTCAGTGTGATCCGGGCTACAAGCTGGACGAGACGCGACGCTTCTGCCGCCCGATCTGCAGCAGTGGCTGTGGCAGCAGTCCGCGGCACAACTGCACCGAACCGGAGATCTGTGGCTGCTCCAAGGGCTATCAGCTGACCGACGACGGATGCCAGCCCGTCTGCGAACCGGACTGTGGAATCGGCGGACTCTGCAAGGACAACAACCAGTGCGACTGCGCTCCTGGTTACAATCTGAGGGATGGTGTCTGCCAGGCCGATTGCTATCAGTAAGCTGAAAACTTCATGCTGGGAATACCAGACTAATGGCAATCTTTATTCGGTGCAGGAAGTGCAACAATGGCGTGTGTGTCAGCCGAAACCGATGTCTATGCGATCCGGGATATACCTACCATGAGCAATCAACCATGTGCGTTCCAGTATAGACATTATTACTAAAGAATTGATTTTTATTTCGAAATCCCTTGGGCTTGATCGG
 

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