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CRIMIC CR01427

Gene ACXA

CG17176

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:12922613..12922614	34A2	FBti0210366	86379

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	long band

Images

Construct Design Sequences

sgRNA sequence	CGAATCCCTTTCTGCCGATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTCACGGGCCTACTAAGCAT	ATGTGGGACGCCATAAATCA	CGGCAGAAAGGGATTCGTTT	TTTGCCGTAACTCCTGTGCT

 

Amplicon 5'

Amplicon 3'

GTCACGGGCCTACTAAGCATAAATTTCTTTGAAGACTTTGTAATCCGCCACAGATGGGTCATGACTTTCACCTCGACTTTGTCGGCATATGTTGTGGTATTGGGTGGTAAGTTTAAGAAGTTAAATATGCCCTTATATTATAGTTACACCAGCTACTTGTAGAGTGTTTGTACTAGATTCAATGATAATTAGTCGATCCTTAAAATCCACAACTTCTTTCCATATACACTCAACTATAGTGTTTTCTCTTGTTTTCAGATATAGCCTTTAACACTTATTATTATTACAAATCCAACTGGCCTCTTAATACCTTATACGATGTCTTTGTGCTCTGCATGATCTACATGTTCCTGCCAATACCTTCGAGCAAGGCAGCAGCTCTGCTAGCCATATCTGTTAGTTTAACATATGTGATATATTTTATTCACTTCATGGCGTTTAACGAGCATAATGTTGCTAAATATGTTCATGGTCTGGATATAGTATCAATCGATTTTTTTCACTACCTGGGCTTCAATATGATGGGAATATTCTTCAGAATCATGAACGACACCATGGTGCGCTCGTCGTTCTTGGACCGATACCAGTTCATTACGGAGGAGATTTGGCTACGCCAAGCTCGCCGTCAAGAATCCTTGCTGCTGGATAGCATACTTCCACCACAGATTGCGAAGCCCATTCAGAAAAGCATTAAGGAAAAGATCATACAGCCCGATAACGACTTCTACCATCTGGGCACTTCGCGGACAGCAGAAAACTTCATGTCCATCCAGATTCACAACGATGTCAGTATCCTTTATGCTGATCTGGTGAACTACACCCAATTGACCACAACATTGACGGTGGAGAAGCTGGTGAAGGTCCTGCACGACCTCTACGCCAGATTTGATTTGGCTGCCTTATCTTTCAAGGTGCAGCGCATCAAGTTCCTTGGCGATTGCTACTACTGTGTGGCGGGTTTGGGGGAATCTGATCCTGATCACGCCACCATGGCTGTGTCTCTGGGAATTTCCATGATTGCTAACATTAAGGAGGTGAGGTATGTTGATTACTATGATCCCATTAGGTTTAGAATCTATGATTTATGGCGTCCCACAT

CGGCAGAAAGGGATTCGTTTTTTTTTTAGTTATTTTGCTCTAATTTGGCTTTCATTTTGAGTACCATTTCGTCCGCAGTGTGAATCGTTCTTTGAATATTGGCATGCGAATTGGTGTCCATTCGGGAACTTTGTTTGCAGGTGTAATCGGCAAGGCCAAGCTACAGTACGACATATGGGGTATGGTACGATGCAACAAATCAATGACACAAAACATATTCGTGAGTTTTCCAGGTGCCGACGTGAATATTGCCAGTCGTCTGGAAGCAACCGGAAGTCCCGGGTATGTGCATGTTAGTGGACGAACTCTGAGCAGCTTGAACGCTGAGGAATACAATATATATCCGGGCACGGAATCGGCCCAGAAGGATCCCGTACTGCAGAAACATCCGATGAGCACCTACCTCCTCACCGCTATACCATCCCTCGATTCGGATAAAACAATTAGCATAGTTGAGGGCGTTCCTAACCTGGACCTTCAAACCGTGGGATCTAACCGAAAATCACAGATTTTGAAGCCTAACTTACTGTCCCATGAGATGCGTGAGGAATTTAGAAAGATGCCGGTGGGGGGTTTCAAGTGAGTTGTGGGGAAAACTGTGCATTGTGCACCTGCAATGAAATAACGAACTACTACCGCAGATTTCAATTCCCTTGGTCGCGAAGAGAAAGAAATTTGAAAAACGAGAAAGTCGAACGCGATTTGGGCATTTTTTGTGTCGCATTTAAGGACAAATCTGTGGAATGGAATTATTTGCATCAGCCCGATTATATTTTTAAATATTCAGTGGCGCTCGGCTGGGGAATTGGTTGCTGTCTTATTTATATCCAGTCCGTGAACAACTCAGATATCTTTTATACGGGTGTTGTCATTAACATTATCGCGTTTTTCGTCCTGACTTTCCTACTATTCATTTGCTGGTACAAGAAAGTGTGCTGGTGGCATAGTGGACAAAACGAGCACAGGAGTTACGGCAAA

 

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