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CRIMIC CR01428

Gene nop5

CG10206

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:6917215..6917224	27C4	FBti0210367	86380

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GCGAAATTGGGTTTACAATCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCAGGACCAGCAGCAACTTA	TCAGGCTATCTCTCGATTGTTCA	CCAATTTCGCTCAGACAGCC	CAAGAAGCTGCTCGTTGACG

 

Amplicon 5'

Amplicon 3'

CCAGGACCAGCAGCAACTTAAAATGACCATGCATCTCGCATAGTTTTCTCGTTCAAATGGCACCAGTTGCAATCGCATTTATATACCACGCCGTTGACCACAAAATGGTCAAAATGACTGGGCCTGGCATCATGATGCAAGCAATTGGGGCTGACGTAAAAACTTTCTGCTCCATTGGTCCCTTTACCGCAGTCAAACGTACTTTTTACCTCGCAGCGAGTGACGGATTGGCCTCCAAAATTGGGAGCTGCTTTTGAAATATGTTGCTAAGCTTCGCAGACAAGAACTGAATGCCTAAGAGGTAGCTTAGTTCTTAAAATCAAAGGACATTAAACAATTGCAATTGCTTTCTTAAGAATTAAGTATATCAAAACCAATACGAATAACATATTAACATTTGTATTTTGTTATGCAAATATTACATTTTATTTCTAATGTACACCGGAAACTTTAATCGTTTTGGTAATTATAAAATCCTATACTTTACAGTAAGGTAACCTATGATGCTAAAACTTAAGGCCTACTAACTAGTCAAGGTGGGAAATTACTCCTGGTGCTTTGACTTTTTCTTTTTCTTTGCTGGTGTTGGCTGATCCTCGGGCTCTGGTGCTGCCGCCTCCTCAACGGCCTCCACCTCTTCGTCCTTCTGCTTCTTCTTCTTTTTCTTCTTCTTCTCGGACTTGACCTCCGCCTCCTCCTCAGCAGGTTCCTCCTTCTTAACAGGCGTCTGCTGTTCGGACTCGGAGTGCTTGCGCTTCTTCTTCACGTTCAAGGTGTTGTCGGCCTCTGGTTGGTAGGTGAACACCTCGCTAAAAAGTTAAGAAATAAATTAAATACACTGAACAATCGAGAGATAGCCTGA

CCAATTTCGCTCAGACAGCCAAGGTTTATCGTCATCTTAATCGGATACATGCGAAACCTTTTGGGTATCATCACTGGAAATCTGTTTACTCTTATATAATTGACAGCTAAAACATACCTCTTGGCCTGGTACTTCTCGAACTTGGCCTTGGCCTTGCCGGTGCCGGAGAGTTTGCGCAGGTTGCCCTCCTCCAGGAGTCGCAGCCGAGACTCCAGTTTAACCTTGTGAGCCGCTCCTAGCTCAAAGGTAGCCTCTTCGCCAAAGGCATCAACACGCGTGGCAAGCGACGCTTTGGCGGCCAGTGAACGCGACATTTTGCCCTTGTTCTTCTGGCTTGCCTGTCCCACCAACTGGGCGTGATAGATCAAACCGTACTTTGGAGTATCCTTCTTGGTCTTCAGCGCACGGAAGAGTGCTTTCTCGGCGCCCAGAATTTGTACAGTAGATGAGGGATGCTTGGCCAGGTTAATCAGCGAGCCAGCATGGGCAATCAGTCGAGCGCCGACGGTGTCGCCCACGAGCACTGTCAAATTCGGAGCCATGGCCATCATTCTGGCCTTCAAGTAGTCGTACAAGTGGGTGCGGTAATCGTTGATCGATATGATCTCGTCGCACAGACACTGAATGTTCAGCACATCCTCCTCGGAGATTTCGGTACCCATAGAGATCTCAGCTGCCTCCTTGACCTTTTCCTCCACATCTTCTGGCAGAATGTCGGACAGATCGCTTGTGGCCATATTATCCCTGGTGCCCACCAACTTGATGGTCTTCACGAAGGCAATGTTGTCGGTAATAATCTTGCCCAGCTCGGGAAAGTGCCAACCGTACCACTCGCGTGCACGCATCATGTAGTTGTTCAACTCCTTGTCCAGGTCATCCAGCAAGCACTGGGCCTGCACAATCATTGTGTCGATCTTGTCGGGCGAGAACTTAAGCTTGTAGCGCGACAAGGAGTGGGCAAGACCCAGGGCCATGGCGGTCATCTCACGCTTGGGCAGACCACCAAGCAGACTGTCCGCCTGCTGGCGAATACAGCGCATCAGCTCCTGGACGCCAGTGTTGCAGACACACTGTACCGACAGTTTGTCCTTGATGGCTGTGCCCAGTTTGGCATCGGCAACCAAGAGCGAAGACTGCACGTCGTCAACGAGCAGCTTCTTG

 

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