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CRIMIC CR01429

Gene CG15256

CG15256

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:15550689..15550701	35D3	FBti0210368	86381

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	GGGAAAATGCGAGCAGCTTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGAGCCACATGAAGACCACC	GCTGCTCGCATTTTCCCAAT	TGTTTCGATACCCAATCACACCT	TCATACTCCTGTCTGCCGGA

 

Amplicon 5'

Amplicon 3'

TGAGCCACATGAAGACCACCGCCATCCCCGATCGTCGTGCGTTCATTTTCTGCATCACTTCCGTTTCCGCCAATTCCCCTCGACGGCCATCGCATTATGTTGTTGAACATTCGGCTTGGTGTCATCGACGACCCGATGTCGACTGTTGATACAAGGCGTCTGATATGGGTAATCACGGGAATAAATACACTCCCGGCCAAGTCTTTATGTCTCGCTTGTGACTGGGGATAATACAGTTTTTAATACAGTCACATTCATAACTACTTAAATCGTCAGATTGCTCACGGATTACACTTTTTAGTAGGATGATTTTTCATATTAATTAAAAAATTCAAAATTTTTTAGTTTCTTGAAGAAAGCTTTTGAAATAATTTCTCATAGTGAAATTGCTTTGTAATGCTACTGGCGCCGATGTATCTACACACCACCTAATCGCAACAACAGCTCGCACACATTCATGCATGGACACTCGTAGTCGGATGAACACAATATTTGTTTAATTAGCTCGTATTAATGCCGTTGCAAGTGCAAATAGAGGTGGCAGATCATTATGGGAGACGCCAAAGAGCCAATCAGCCATTGTTATTGGCCATTGTAGTCGGGTTGGCCACTAAACACACCGGAACTCACCGCCCACACCGCCCATAGCGCCCCCTCTTATTTCGACTCCTGTGCATTTCCACCGATGACCCACTATTCGATTTTCATTTTCATCGGCTATTGATTCAGAAATCATTTGCCTCCGCCCGCAACAAATCCGCTTGTGAATTCAATTGTGGCGCTTATAATATTGTGCAACGACTGCCAGCACCACGCTGGCAGTTTAATGATGTGATTTCTGTTTTTGTGCACTGGGGATTATGGCGAGCTGGGCTCAAAGGTTGAAAGGAATTCACTCTGAGAAACATTTCAGATCGCTCAGCAAACTTCGTTCACATTGTTACTCACTTTTCCGACACGCACACAATCACAGTCAAAATGGCCTGCAAAAAGAAAACCAAACTGTGGGAACCAATGACCAAGGATGAATTGAAGTCCACCACACAGGCGATGCTCGAGGAGGGCGCCAGGGGTTTAAGGACGATTTCTACGCAGCAGAAAAAGCTGGTCAACTTTCAGTCGTTCAACATGGATGAGTGAGTGGAAAATTATGCTGTTTAGGGATATAAAGGGTATATTGGGAAAATGCGAGCAGC

TGTTTCGATACCCAATCACACCTATATATTGGGATTGTGGATTACTGTGTGATCTTTCACCTTACTTTACTTTTCCGCAGTCCCCGTTACGGCATTTCCCTAATCGAGGACAACATGGAACCGCCCATGTTGTCGAGCTACACAAGGCAATACTCCCAGCCGTATCCAAATCGTTGTAAGCCCTTTGTTCAAAAGACGGAAACACCAGATCCCATATTCAATCGAAGGCCGAAAAACGATTTTGAGTTTACCACAGGACTGGATTTTAAGTTTCTTGATGATCACATGCACAATCTTTCCGAATCACGCAAGAAAGTCAACTTCTTCCGGCAACTGAGGTAAGAAAAATAATCTTAATTTTAATTTATAATATTAAAAATTTCTCGTGATACACAGGAATCAATCATTTCTTCTGTATTGACATGGTTAACAATTTTTCGAGCTAAAATTACAAAACAAAAACTATTAATTTGTAACAATTTGACTCCATCATCGGCAAAGTTACGCAACGTGGCTCCTATTTTAAGAGCTTTCAGTTGTGCCATGATTATAAATTACCCCTCATTATGTTCCGGGAAAAATGTGTCAATTGCATTTTCCATTACCCCTTCGTTTTCGATAGAAATCAATTAATTTTGCATAAATAAACTCGATTGGCAGCGTGGTCAGGGGAGAGCCATGTGCTTGACTATTATTAGGTTATAGCCGGTACACTTTTCTTGGAAAAATAGCAAGCAAATTGCAATTAATAATGCAAAAGGCAGCAAGCCAAGTCCGATACTCGGCCATTGCTGCGATATTCGCCGCGGGCACTGGCATTGCTGCCAATTTGCATAAATGCTAGAAGAGCTCTCCCGTTGGCCAGTGGGACGTCGGGCAGGAGGGTGGTGTCAAACTAATGGCAGTCTATGACAGGGATCTGATTTATTATGCACGGTAATTAGTTTGGCCTCCTTAGTGTCCGAATGGAAGCCAATAAACCAGGCTGACATGCCGCTGCATTGATTTTGCCCCGAGGAAAGCGCGCATAAGCGATGGATTTCCTTCTACCGTCCAGCACCGCAAGCATCGCCCCAATGGAGTGGCCAAAACATAACAATCTCCGGCAGACAGGAGTATGA

 

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