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CRIMIC CR01430

Gene CG5050

CG5050

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:17455452..17455455	36C9	FBti0212925	91253

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GATTGCATATGCTGGAGGAGCAACTG	CGGGAATCTACACCAATGTGGCAATG

Images

Construct Design Sequences

sgRNA sequence	GTTGTATACCCGCCGTTGTTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACACACCAGAGTTCTCCCCT	ACGGCGGGTATACAACAC	TTCGGTTCGGTTGCTGGATT	AGGCATTTCACTCGGCGATA

 

Amplicon 5'

Amplicon 3'

ACACACCAGAGTTCTCCCCTCCGATTGAACAGAATAGGGCCGATCCTCCATCACCGGTGCAGGTATCGCGGCCTAACTCGCCTCCGGCGCAAATGATGTTCTTGGGCAGCTCAAATTTAGCGCCCAACCTGGTCGACCTCAAGAACTTCTCGCAAACATTTCGATTTACCACGAGCAACTGAACTTTCTTCAGGACGGTGGAGTATCGTTGATCCTCGTATCTCATTTTTCCCCAACCGGCCACCGTGCAACGACGTCCTGCGAACGATTTATTTGGTGTAGGCAAACAGATAGTTCTGATGTGATCATTCAGTTTGAAGGGCGAATTTAGGAAAAGTAACGCCAAGTTGTTGGCACCTGATTTGAAGTCAAATCCTTCATGGATTACTGCCCTTTCAACTTCGCGCTGCTCAGACAAAAATATCTCTCGATCGGATTTTAAGTCCCAATCGCCGGCCCTGACCACAAGTTCGGTTTCGATTGTAATTACCCGGTGAGCTACTGTTAGAACGACATTTGGTTGAATCAGGGATCCGCCAGCCAAGTACTGTCCATTGTGAAAAATAGCAACAGCCCAGGGATATTGAGCTGGTCGTGCTTGATCAATGGTTATTCCTTCTACCATTTGCAAGCCATTTGCGTTGCTCATGCCGCAACTTGGTGACAAAAGCTCTGAGCTTCCAACATTGGTAGTCGGGTTCCCTCCGTTGTTTGTTGGATTTCCAAAATTGGTAGTCGGGTTCCCTCCGTTGTTTGTTGGATTTCCAACGTTGGTTGTTGGATACCCACCGTTGTTTGCTGGATACCCACCGTTGTTTGCTGGATACCCACCGTTGTTTACTGGATACCCACCGTTGTTTACTGGATACCCACCGTTGTTTGATGGATACCCACCGTTGTTTGATGGATACCCACCGTTGTTTACTGGATACCCACCGTTGTTTACTGGATACCCACCGTTGGGTGTTGTATACCCGCCGT

TTCGGTTCGGTTGCTGGATTCCTTTCGTTGTTGGTTGGACGCCCACTGAAGTCGTTGATTTCCTTTACTTGTGACTCAATGGTATTTGGGCGATCTCTAGTGAGTTCGCGTGTAGGCGATAGTTGTGCATTTATTCCGTTTACTCCAACCTTATCATCTTCGGGGCCATTTGGTTCAATTAAAACCGTACCTCCAGGCTTTTATCCGTCATAGAAAATTTGCACTTAGTCAAGCTAACAATAAATCGCATTCAACAATTGTATAAGCTTACCTTTCGTAGAACTGCACAGCAAATTTTGGTATTGCCGCAATTACTGGAGCGGTAATCAAATGTTGGTTCCTCATACAAACAAAAATTCCGGGTAACGCAAGTATGTCGACAGGCATTGCCCATAAGGACACTTGCTAAGAGTAGAGTTCCGATTGCCCAACAATGCTTCATGTTTGCTTCAGAAAACTGAGTGAACGAACGGCTGGCAGTGGGTATCATTATATTTCTTTGCTGAGCAGCTTAAAAGCTTATCAGCCGACAAAGATGTATTCGAAGTACATATGTACATGTACATATGTACATGCATATGCACATAAATTGTGTTATTCCCAAAGATTGCACAGAAAACGCTTAAGTTAAGAGATTGCGAAAGAGATATTTCCGAATTCTTTGGAAATATCCATTAAAGTTTTCAAAAAAAATGTTCAGATTAATTAAACTGGTAACGATCATTCCCAGGACTATACATATATATCTCACAGGATTTGATACGCTTAATTCTAGCTGTTACATACCTTTTAACTAATCGAGTTATGTATCTACCCTTTACCTTTTCGAGTAACGTGACCTGTATAACACGTAATAAAGTTTCAATATAAAAAAAATTTTTTTTTTATGTACGCATATATAAAAAAAAAACATAAAGAGGAAAGAACTACAAATATGCGTACAAAAGAATTATCATCTGAAATATGTACACATTTATATACATAACACTGACCTTAGGATGCATGGATTATCGCCGAGTGAAATGCCT

 

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