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CRIMIC CR01431

Gene CG4267

CG4267

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:2243044..2243057	22D5	FBti0210369	86382

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK

Images

Construct Design Sequences

sgRNA sequence	TACACTGAACGGTGAGATAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTATGCGTGCCAATTAGCGC	TCTCACCGTTCAGTGTAATGAA	GCTGTACAAACCTTTCAGCTCG	ATCGATTCGGTACTCGTCGC

 

Amplicon 5'

Amplicon 3'

TTATGCGTGCCAATTAGCGCCAGAAGGGCTTATGGTTTTGTATAATCATAACAATTTGTCGGTTTTTTAAGGATATTTACTTAAAACTGCATAATACACTTATAATTTTCATGAGTTTTTGTTGTTTTAAACAGATAACAGCACTTTATATTTCTTTATACCTCTAATTTTCAAGATCATCAGCCTGCTAACCTAATCGATACTCTCTATAAGGAAGTATAATGATCTATGAGGAGAGAGAGAGAGAGAGAGAGAGAGTGAGCGAGCTGCGATGATCAGTTATCACTGGAGTGAGTAACCCACTGAGCGTAGACTTGGGGTCGCCGCGCTCTCCGCTCCGATTCGGAAAGTGGAGCTCGCAACGCTCGACCATCGAGAGATTGGCGGAGAGCTGTGTCAGTTGGTTGCGCACGCACCAGTCGACCGGTCAGATCTGCGGCTTATTCCGCCGGCGTTCGAAATTTATAACGGTGGAGATCGGTCATCGGACGCACTACACTCGATCGGTCAGAAGCCCCGAGGGAGATATCTTGGAACAGCCATGATCTCGCAGCTGGGACGGATTAGCATCATGAGTGCGGTGCTTTTGGCATTTGCGGCCATCGTATTTGGTGGGTAATATTCACACATTTCAAGGTCTTATCACAGATCTGTGTAGAAATTCATTTGATTGATATATTTTTGGTTTGCTTGCTATATCGTCTTTAACTAGCCAGCAGCTCGGAGAAAACCGAACTCGATCCCAATGCCACCTGCAACTACACGCTGGTGAAGAAGAAGACCTTGGGGGTGGATCCCTCGTTCTGGAAGAAGTTCTTCAAGCACCTCATACCCTTCACGAGTTCGAGGGGCAAAATGCAGTTCATACTCTTCAAGCGGGACTTCGCGGACTGCGGAAGGGAACTGTTCGTGGGCGATGTGGAAAACCTGAGGAACTCGGGCTTCGATGCACGTCATCAAACAAGGTAAGGGGGATTTCCGGGAAGATTAAAGCCCCGATCGAATGGGGAACTTTGCAGCGAAGTGAGTGTGCCACAATCAATTCATTACACTGAACGGTGAGA

GCTGTACAAACCTTTCAGCTCGCAACAATTGACAGTGTATTTAGTATTCATACTACCACTAAGCCCCAGAAATTAATGTGAGAGCAAAGCACCAATCTAATGAATAGAACCAAAACAAATATTTGCTAGATTCTCTCACCAAATAATTTATTTTTTATATTTCGCGACTAACACGAAAACAGGTTTCTCTGCTTTATATATTTACATTTTTTGGTTGAGCTTTCGTTTGAATTATCGCCATTGAGTCATTGCCATTGGCCTGACAATTGATTTATTGCTAATTACAAGTTAATTCATTATAATAGCTAACAGATAGTAATTGCACAAATTTACACCAAATACGCCAAGCAATACACCAATTTTGCATTAAAAGATAAGATATCAGGCGAAGTTGATATCATATATACATAAATGAATTGAAATATGTTTATTTTAAAGGGAATTGCTATTAGTTTAGTATACATTGATATATTATATTGACTTTCCTGACTGCTTATTTTTAGGATAGTCATTCATGGCTGGATGAGTCAGAGCAAAGGTTCGCATATAAGGAAGGTTAAGAACGCATACCTCAGCCTGACCGATCCGGGACCCAACGGAGAGCCCGCCCCCTACGAGGACTTCAATGTGATCGTATGTGACTGGAGCAAGACTTCCACGAACGTAAACTACTACGAGGTGGCCAAGACGGTGGAGGACATGGGAGCCCTGCTGGCGGAACTGGTGCGCTATCTGAACCAGGAGGCGAACATGCACTACGACGACGTATACGTCATTGGACACTCTCTGGGAGCGCAGATCGCCGGCAGTGCTGGCAAGCAGATAATGCCATATCGATTCAACACCATATACGCTCTGGATCCGGCGGGTCCACAGTTCCGGGAGAAGAGCGACGAGTACCGAATCGAT

 

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