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CRIMIC CR01434

Gene CG14752

CG14752

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:8591499..8591521	44D4

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TCTTTACCACCGACCAGAGGGTCTTA	AAACGGCGAGTATGACGTCGGATACT

Images

Construct Design Sequences

sgRNA sequence	ACTGATTAGCTAACAGGCGATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTTCGCTTGGCCGTTGAAG	TGCTTGAACCCAGCGTCTT	GGTACATGGCATTCGATCTAACC	GCTTCACTTACCCGACTCCC

 

Amplicon 5'

Amplicon 3'

TTTTCGCTTGGCCGTTGAAGTTGCTGCTGCTGTTGTTGCTGCTGCTGCTGCTGCTGTTGTTGCTGCACCGACGTCACGTCTTTCTCGTGCAGCGCAGTCGGCTTGCAGCAACAACACAAAGCGCTGCTGGCGCCCGGTATAAAACGGATGAGTCGCAGCCTGTGGTTATCAGTCACCACCTGACAGTGCTCCGGTTTTAGTATCGCCTTTCGAGTGCAGCAGAGCTCAGCCCAGCCACCATGTCCGTCCTGAAAGTATTCGTAAGTGCGACAAGGATAACGTTCGAAAAAGTGTTAAGCCAAACCTCCGCGGAATGGAGCTGAAAAGGATTAAACTCATGTGGACTATCGTGAATAAAACTAACCAACTGAAACATAACAAACTCACTGAAAACTGACTTCGATAACCGAAGTATTGGAAATATACCACCAAGATCGCCTGATGGCTTAAAAAGTCTCGAATAGATATTTCAAGAAACTGCGAAAGCATTTATTTTGCAGAAATATAAATTCACCCAAAAATATTAGTTTATGTTTATCAATCGCGGACTAACATTTCATTAGATTACTTAGATTTCACCAGAATTGAACATAAATTTTGAAGTGCCAAATTAAAACTAATCACACTGATTAGTTAGCTATTTGCGTTAATCTAGCCGGACCTAACTTACTAACTTCCTTATCCCCTTTCAGATCTTCGTCGCCCTCTTCGCCGTGGCCTACTGCGCACCCAGTCCCGGCTTCTTTGGCAAACAGTGAGTATCGGCGGCGATCTGCACCTGAACTGATCCATAAATAACCCGAGCGAATCGATAAAAATACCCATTTCCCGCTTTATGCTAAGCTAACGAGCGGTCGCATGGCTTTTTAAACGCTGCGACTGCTCCGTGGGTCTATGAAATATGTGTCCGATTGCGATCGAATTGAGCAATCAAAACGCTTGTAGCACTTAGAGCCCCAGCCCCCTCCAGCAGTCTCTATATGGTGCCCACCACTGGCTACCATTGAACTTAGCCGATCTCGACTTGGCTTGGCTCGCGGCTTAGGAATTTCGCAGGCGGCGGTTGGCATTTAATTCAGCGTAATGCGCGCATAATTGTGCGAATCAATTGCCAATTGCCAATAGCCAATATGCAATAGCCATGTCTGCCAAGACGCTGGGTTCAAGCA

GGTACATGGCATTCGATCTAACCGATTTCACTTTCGATCCGCAGCGAGCACCACACCATCCACGTGCCCTACAAGGTGCACACGGTGCACCACCATCACGTTCAGAAGGTCCATGTGCCGGTGGTGAAGCACGTGCCGGTGCCCATTTACAAGGAGGTGCCCGTGCACCACGTCCACCACGAGGAGATCCCCGTGCCGGTGCACCACGTCCATCACGAGGAGATCCCGGTGCACCATGTCTTCGATGACCACCACGACCACCACGGCTGGAGCTCCCACCACGGACACGGCTGGCTGTAAGCCATCTGCCCGCTGGCCTGCTTGTCTTCAAACCCACCCACTCAGCCAGTCCCATCTGAAGCAGCCTGTAAATACTGTACAATGCTAAGTTAATGAATGTAAATAAATCACAATTCGACTGTACACAGAACAATCCTGGCGTATTTATGGCAGGATTCCCTGAAGTGGAACCTTAGTGTTTGATGGCTCACAAGTAATCTAATAAACCAAATACTTTTAATGGGAATGAAATAACGATTTTGTTATTTGAGGCTTTTTAAGTTGTTGCGATTAGGATAGATGGTTACTTTTGAAGACTCGAAACTTGCTTATCTCTATATTTATATACTATATAAGATGGTAGAATATAACCTACTTGTTAAGTAATATTTTTCAAAAGATTTTTGTTATCAGTGACAGAACTTGTACACATAAAATAAAAACCCCATATACTCAGATACTCAAATACTAATACCCTGATAGCAAATAGCCACAAATAGACACACTACCACATTTGCACCATGATATATCTACAGAGTTGCTGCTGCTGCGTGGATCTGCGAATCGGAACCATAGCAATAGGGATTTTGCACATTATAGCGGATATCCTTGGAGGCACATTTGTTGCTTTATTTGGGGAGTCGGGTAAGTGAAGC

 

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